High-throughput approaches for screening and analysis of cell behaviors

Jungmok Seo, Jung Youn Shin, Jeroen Leijten, Oju Jeon, Gulden Camci-Unal, Anna D. Dikina, Katelyn Brinegar, Amir M. Ghaemmaghami, Eben Alsberg, Ali Khademhosseini

Research output: Contribution to journalReview articlepeer-review

28 Citations (Scopus)

Abstract

The rapid development of new biomaterials and techniques to modify them challenge our capability to characterize them using conventional methods. In response, numerous high-throughput (HT) strategies are being developed to analyze biomaterials and their interactions with cells using combinatorial approaches. Moreover, these systematic analyses have the power to uncover effects of delivered soluble bioactive molecules on cell responses. In this review, we describe the recent developments in HT approaches that help identify cellular microenvironments affecting cell behaviors and highlight HT screening of biochemical libraries for gene delivery, drug discovery, and toxicological studies. We also discuss HT techniques for the analyses of cell secreted biomolecules and provide perspectives on the future utility of HT approaches in biomedical engineering.

Original languageEnglish
Pages (from-to)85-101
Number of pages17
JournalBiomaterials
Volume153
DOIs
Publication statusPublished - 2018 Jan

Bibliographical note

Funding Information:
This paper was supported by the National Science Foundation ( EFRI-1240443 ), the Ohio Biomedical Research Commercialization Program ( TECG20150782 ), an ONR PECASE Award , the Department of Defense Congressionally Directed Medical Research Programs ( OR110196 ) and the National Institutes of Health ( AR066193 , AR063194 , AR053733 , AR069564 , AR007505 AR061265 , AR057837 , DE022376 , DE021468 , DE025899 , EB023907 ). Dr. Seo was partially supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education ( 2016R1A6A3A03006491 ) and KIST project ( 2E26900 ). Dr. Leijten acknowledges financial support from Innovative Research Incentives Scheme Veni # 14328 of the Netherlands Organization for Scientific Research (NWO).

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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