High-throughput biomimetic 3D gel-island chip for investigating cancer cell heterogeneity

Zhixiong Zhang, Yu Chih Chen, Yi Luan, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Considerable evidence showing that ECM is an important regulator of cellular behavior including adhesion, metastasis, proliferation, and differentiation. Although hydrogel has been used in the cell culture, previous works focus on the collective cell behaviors rather than single cell heterogeneity. This paper reports a novel gel-islands microfluidic platform enabling single cells tracking in biomimetic three-dimensional (3D) microenvironment. The platform has the advantages of (1) reliable single cell encapsulation in 3D gel-islands, (2) high-throughput single-cell drug screening assays, and (3) automatic single cell lineage tracking. Combining gel-based 3D culture and single cell resolution, the presented platform can monitor cellular behaviors of hundreds of individual cells in 3D ECM microenvironment using small amount of matrix reagents and drugs.

Original languageEnglish
Title of host publicationMEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages691-694
Number of pages4
ISBN (Electronic)9781509019731
DOIs
Publication statusPublished - 2016 Feb 26
Event29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 - Shanghai, China
Duration: 2016 Jan 242016 Jan 28

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2016-February
ISSN (Print)1084-6999

Other

Other29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
CountryChina
CityShanghai
Period16/1/2416/1/28

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'High-throughput biomimetic 3D gel-island chip for investigating cancer cell heterogeneity'. Together they form a unique fingerprint.

Cite this