High-throughput cancer cell sphere formation for 3D cell culture

Yu Chih Chen; Euisik Yoon

doi:10.1007/978-1-4939-7021-6_21

High-throughput cancer cell sphere formation for 3D cell culture

Yu Chih Chen, Euisik Yoon

Yonsei-IBS Institute

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

Three-dimensional (3D) cell culture is critical in studying cancer pathology and drug response. Though 3D cancer sphere culture can be performed in low-adherent dishes or well plates, the unregulated cell aggregation may skew the results. On contrary, microfluidic 3D culture can allow precise control of cell microenvironments, and provide higher throughput by orders of magnitude. In this chapter, we will look into engineering innovations in a microfluidic platform for high-throughput cancer cell sphere formation and review the implementation methods in detail.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	281-291
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-4939-7021-6_21
Publication status	Published - 2017 Jan 1

Publication series

Name	Methods in Molecular Biology
Volume	1612
ISSN (Print)	1064-3745

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All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

Cite this

Chen, Y. C., & Yoon, E. (2017). High-throughput cancer cell sphere formation for 3D cell culture. In Methods in Molecular Biology (pp. 281-291). (Methods in Molecular Biology; Vol. 1612). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7021-6_21

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Access to Document

10.1007/978-1-4939-7021-6_21