Abstract
Three-dimensional (3D) cell culture is critical in studying cancer pathology and drug response. Though 3D cancer sphere culture can be performed in low-adherent dishes or well plates, the unregulated cell aggregation may skew the results. On contrary, microfluidic 3D culture can allow precise control of cell microenvironments, and provide higher throughput by orders of magnitude. In this chapter, we will look into engineering innovations in a microfluidic platform for high-throughput cancer cell sphere formation and review the implementation methods in detail.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 281-291 |
| Number of pages | 11 |
| DOIs | |
| Publication status | Published - 2017 Jan 1 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1612 |
| ISSN (Print) | 1064-3745 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
Cite this
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High-throughput cancer cell sphere formation for 3D cell culture. / Chen, Yu Chih; Yoon, Euisik.
Methods in Molecular Biology. Humana Press Inc., 2017. p. 281-291 (Methods in Molecular Biology; Vol. 1612).Research output: Chapter in Book/Report/Conference proceeding › Chapter
TY - CHAP
T1 - High-throughput cancer cell sphere formation for 3D cell culture
AU - Chen, Yu Chih
AU - Yoon, Euisik
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Three-dimensional (3D) cell culture is critical in studying cancer pathology and drug response. Though 3D cancer sphere culture can be performed in low-adherent dishes or well plates, the unregulated cell aggregation may skew the results. On contrary, microfluidic 3D culture can allow precise control of cell microenvironments, and provide higher throughput by orders of magnitude. In this chapter, we will look into engineering innovations in a microfluidic platform for high-throughput cancer cell sphere formation and review the implementation methods in detail.
AB - Three-dimensional (3D) cell culture is critical in studying cancer pathology and drug response. Though 3D cancer sphere culture can be performed in low-adherent dishes or well plates, the unregulated cell aggregation may skew the results. On contrary, microfluidic 3D culture can allow precise control of cell microenvironments, and provide higher throughput by orders of magnitude. In this chapter, we will look into engineering innovations in a microfluidic platform for high-throughput cancer cell sphere formation and review the implementation methods in detail.
UR - http://www.scopus.com/inward/record.url?scp=85021327532&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85021327532&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7021-6_21
DO - 10.1007/978-1-4939-7021-6_21
M3 - Chapter
C2 - 28634951
AN - SCOPUS:85021327532
T3 - Methods in Molecular Biology
SP - 281
EP - 291
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -