High-throughput cancer cell sphere formation for 3D cell culture

Yu Chih Chen, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)


Three-dimensional (3D) cell culture is critical in studying cancer pathology and drug response. Though 3D cancer sphere culture can be performed in low-adherent dishes or well plates, the unregulated cell aggregation may skew the results. On contrary, microfluidic 3D culture can allow precise control of cell microenvironments, and provide higher throughput by orders of magnitude. In this chapter, we will look into engineering innovations in a microfluidic platform for high-throughput cancer cell sphere formation and review the implementation methods in detail.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages11
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
This work was supported in part by the Department of Defense (W81XWH-12-1-0325) and in part by the National Institute of Health (1R21CA17585701). The Lurie Nanofabrication Facility of the University of Michigan (Ann Arbor, MI) is greatly appreciated for device fabrication.

Publisher Copyright:
© 2017, Springer Science+Business Media LLC.

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


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