High-throughput cancer cell sphere formation for 3D cell culture

Yu Chih Chen; Euisik Yoon

doi:10.1007/978-1-4939-7021-6_21

High-throughput cancer cell sphere formation for 3D cell culture

Yu Chih Chen, Euisik Yoon

Yonsei Advanced Science Institute

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

Three-dimensional (3D) cell culture is critical in studying cancer pathology and drug response. Though 3D cancer sphere culture can be performed in low-adherent dishes or well plates, the unregulated cell aggregation may skew the results. On contrary, microfluidic 3D culture can allow precise control of cell microenvironments, and provide higher throughput by orders of magnitude. In this chapter, we will look into engineering innovations in a microfluidic platform for high-throughput cancer cell sphere formation and review the implementation methods in detail.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	281-291
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-4939-7021-6_21
Publication status	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1612
ISSN (Print)	1064-3745

Bibliographical note

Funding Information:
This work was supported in part by the Department of Defense (W81XWH-12-1-0325) and in part by the National Institute of Health (1R21CA17585701). The Lurie Nanofabrication Facility of the University of Michigan (Ann Arbor, MI) is greatly appreciated for device fabrication.

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-7021-6_21

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Cite this

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