High-throughput cancer cell sphere formation for 3D cell culture

Yu Chih Chen, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Three-dimensional (3D) cell culture is critical in studying cancer pathology and drug response. Though 3D cancer sphere culture can be performed in low-adherent dishes or well plates, the unregulated cell aggregation may skew the results. On contrary, microfluidic 3D culture can allow precise control of cell microenvironments, and provide higher throughput by orders of magnitude. In this chapter, we will look into engineering innovations in a microfluidic platform for high-throughput cancer cell sphere formation and review the implementation methods in detail.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages281-291
Number of pages11
DOIs
Publication statusPublished - 2017 Jan 1

Publication series

NameMethods in Molecular Biology
Volume1612
ISSN (Print)1064-3745

Fingerprint

Microfluidics
Cell Culture Techniques
Cellular Microenvironment
Neoplasms
Cell Aggregation
Pathology
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Chen, Y. C., & Yoon, E. (2017). High-throughput cancer cell sphere formation for 3D cell culture. In Methods in Molecular Biology (pp. 281-291). (Methods in Molecular Biology; Vol. 1612). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7021-6_21
Chen, Yu Chih ; Yoon, Euisik. / High-throughput cancer cell sphere formation for 3D cell culture. Methods in Molecular Biology. Humana Press Inc., 2017. pp. 281-291 (Methods in Molecular Biology).
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Chen, YC & Yoon, E 2017, High-throughput cancer cell sphere formation for 3D cell culture. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1612, Humana Press Inc., pp. 281-291. https://doi.org/10.1007/978-1-4939-7021-6_21

High-throughput cancer cell sphere formation for 3D cell culture. / Chen, Yu Chih; Yoon, Euisik.

Methods in Molecular Biology. Humana Press Inc., 2017. p. 281-291 (Methods in Molecular Biology; Vol. 1612).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Chen YC, Yoon E. High-throughput cancer cell sphere formation for 3D cell culture. In Methods in Molecular Biology. Humana Press Inc. 2017. p. 281-291. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7021-6_21