High-throughput detection of human salivary cortisol using a multiple optical probe based scanning system with micro-optics and nanograting coupled label-free microarray

Eikhyun Cho, Seoyeon Choi, Jongmyeong Shim, Taekyung Kim, Ryung Shin, Jinhyung Lee, Jongho Kim, Hyo il Jung, Shinill Kang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We demonstrate the use of a parallel detection system with a nanograting-based microarray to accomplish high-throughput analysis of bio-molecular interactions in a label-free manner. Well-type label-free microarrays were fabricated to eliminate the risk of cross-contamination and to minimize sample volumes. Parallel analysis without the use of spectrometer arrays or a moving platform was accomplished by using scanning multiple optical probes generated by a spatial light modulator and microlens array. Additionally, multiple optical probe spots focused by the microlens array reduced detection errors while enhancing the signal-to-noise ratio within a high-density microarray. Finally, we verified the feasibility of the parallel detection system by analyzing the peak wavelength value (PWV) shift of human salivary cortisol and anti-cortisol in a competitive binding experiment.

Original languageEnglish
Pages (from-to)520-527
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume233
DOIs
Publication statusPublished - 2016 Oct 5

Fingerprint

Cortisol
Microoptics
Microarrays
Hydrocortisone
Labels
Throughput
optics
Scanning
scanning
probes
Molecular interactions
Error detection
molecular interactions
light modulators
Spectrometers
Signal to noise ratio
contamination
Contamination
signal to noise ratios
platforms

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

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abstract = "We demonstrate the use of a parallel detection system with a nanograting-based microarray to accomplish high-throughput analysis of bio-molecular interactions in a label-free manner. Well-type label-free microarrays were fabricated to eliminate the risk of cross-contamination and to minimize sample volumes. Parallel analysis without the use of spectrometer arrays or a moving platform was accomplished by using scanning multiple optical probes generated by a spatial light modulator and microlens array. Additionally, multiple optical probe spots focused by the microlens array reduced detection errors while enhancing the signal-to-noise ratio within a high-density microarray. Finally, we verified the feasibility of the parallel detection system by analyzing the peak wavelength value (PWV) shift of human salivary cortisol and anti-cortisol in a competitive binding experiment.",
author = "Eikhyun Cho and Seoyeon Choi and Jongmyeong Shim and Taekyung Kim and Ryung Shin and Jinhyung Lee and Jongho Kim and Jung, {Hyo il} and Shinill Kang",
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High-throughput detection of human salivary cortisol using a multiple optical probe based scanning system with micro-optics and nanograting coupled label-free microarray. / Cho, Eikhyun; Choi, Seoyeon; Shim, Jongmyeong; Kim, Taekyung; Shin, Ryung; Lee, Jinhyung; Kim, Jongho; Jung, Hyo il; Kang, Shinill.

In: Sensors and Actuators, B: Chemical, Vol. 233, 05.10.2016, p. 520-527.

Research output: Contribution to journalArticle

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AU - Cho, Eikhyun

AU - Choi, Seoyeon

AU - Shim, Jongmyeong

AU - Kim, Taekyung

AU - Shin, Ryung

AU - Lee, Jinhyung

AU - Kim, Jongho

AU - Jung, Hyo il

AU - Kang, Shinill

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AB - We demonstrate the use of a parallel detection system with a nanograting-based microarray to accomplish high-throughput analysis of bio-molecular interactions in a label-free manner. Well-type label-free microarrays were fabricated to eliminate the risk of cross-contamination and to minimize sample volumes. Parallel analysis without the use of spectrometer arrays or a moving platform was accomplished by using scanning multiple optical probes generated by a spatial light modulator and microlens array. Additionally, multiple optical probe spots focused by the microlens array reduced detection errors while enhancing the signal-to-noise ratio within a high-density microarray. Finally, we verified the feasibility of the parallel detection system by analyzing the peak wavelength value (PWV) shift of human salivary cortisol and anti-cortisol in a competitive binding experiment.

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