High-throughput detection of human salivary cortisol using a multiple optical probe based scanning system with micro-optics and nanograting coupled label-free microarray

Eikhyun Cho, Seoyeon Choi, Jongmyeong Shim, Taekyung Kim, Ryung Shin, Jinhyung Lee, Jongho Kim, Hyo Il Jung, Shinill Kang

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We demonstrate the use of a parallel detection system with a nanograting-based microarray to accomplish high-throughput analysis of bio-molecular interactions in a label-free manner. Well-type label-free microarrays were fabricated to eliminate the risk of cross-contamination and to minimize sample volumes. Parallel analysis without the use of spectrometer arrays or a moving platform was accomplished by using scanning multiple optical probes generated by a spatial light modulator and microlens array. Additionally, multiple optical probe spots focused by the microlens array reduced detection errors while enhancing the signal-to-noise ratio within a high-density microarray. Finally, we verified the feasibility of the parallel detection system by analyzing the peak wavelength value (PWV) shift of human salivary cortisol and anti-cortisol in a competitive binding experiment.

Original languageEnglish
Pages (from-to)520-527
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume233
DOIs
Publication statusPublished - 2016 Oct 5

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea(NRF) Grant funded by the Korean Government(MSIP) (No. 2015R1A5A1037668 ) and in part by the Bio & Medical Technology Development Program of the NRF funded by the Korean government, MSIP (2015M3A9D7067364).

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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