High-throughput microfluidic chip for magnetic enrichment and photothermal DNA extraction of foodborne bacteria

Kirok Kwon, Hogyeong Gwak, Kyung A. Hyun, Bong Seop Kwak, Hyo Il Jung

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have developed a high-throughput pretreatment microfluidic chip for enrichment of microorganisms in food using magnetic particles and extracting DNAs using photothermal effects of magnetic particles. Magnetic particles modified with Concanavalin A can capture a variety of pathogens in the sample. As magnetic particles and bacteria injected into the microfluidic chip at a high flow rate, they bound actively in the mixing channel. After passing through the mixing channel, the combined bacteria and magnetic particles complexes were captured and enriched by magnetic force at the chambers which rectangular neodymium magnets were assembled in the form of dozens of arrays. After the magnet arrays were removed, the elution buffer was injected at a slightly lower flow rate and the eluted particles were captured in a small lysis chamber. The laser with a wavelength of 532 nm was irradiated at the lysis chamber to dissolve the captured bacteria as strong heat generated by the photothermal effects of the magnetic particles. Finally, the extracted DNAs were detected by real-time PCR.

Original languageEnglish
Pages (from-to)62-68
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume294
DOIs
Publication statusPublished - 2019 Sep 1

Fingerprint

Microfluidics
bacteria
Bacteria
DNA
deoxyribonucleic acid
chips
Throughput
Magnets
Flow rate
Neodymium
Pathogens
Concanavalin A
chambers
Microorganisms
Buffers
magnets
flow velocity
Wavelength
Lasers
elution

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

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abstract = "We have developed a high-throughput pretreatment microfluidic chip for enrichment of microorganisms in food using magnetic particles and extracting DNAs using photothermal effects of magnetic particles. Magnetic particles modified with Concanavalin A can capture a variety of pathogens in the sample. As magnetic particles and bacteria injected into the microfluidic chip at a high flow rate, they bound actively in the mixing channel. After passing through the mixing channel, the combined bacteria and magnetic particles complexes were captured and enriched by magnetic force at the chambers which rectangular neodymium magnets were assembled in the form of dozens of arrays. After the magnet arrays were removed, the elution buffer was injected at a slightly lower flow rate and the eluted particles were captured in a small lysis chamber. The laser with a wavelength of 532 nm was irradiated at the lysis chamber to dissolve the captured bacteria as strong heat generated by the photothermal effects of the magnetic particles. Finally, the extracted DNAs were detected by real-time PCR.",
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High-throughput microfluidic chip for magnetic enrichment and photothermal DNA extraction of foodborne bacteria. / Kwon, Kirok; Gwak, Hogyeong; Hyun, Kyung A.; Kwak, Bong Seop; Jung, Hyo Il.

In: Sensors and Actuators, B: Chemical, Vol. 294, 01.09.2019, p. 62-68.

Research output: Contribution to journalArticle

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