TY - GEN
T1 - High-throughput of photodynamic therapy (PDT) screening from multiple parameter assays of 1,000 different conditions in a single chip
AU - Lou, X.
AU - Kim, G.
AU - Koo, Y.
AU - Kopelman, R.
AU - Yoon, Euisik
PY - 2010
Y1 - 2010
N2 - We present a microfluidic chip for high-throughput PDT screening with the capability of individual controls on light exposure dose, pericellular oxygen level and photosensitizer concentration. Both oxygen and photosensitizer gradients are produced by microfluidic gradient-generating networks and imposed on target cells. Various photo-exposures are provided by orthogonally stacking the two layers of gray scale liquid filters. All three parameters are independently controlled in the microfluidic channels monolithically integrated in a single chip. Using this platform, we successfully demonstrated the PDT efficacy on cancer cells for different exposure doses, oxygen levels and photosensitizer concentrations.
AB - We present a microfluidic chip for high-throughput PDT screening with the capability of individual controls on light exposure dose, pericellular oxygen level and photosensitizer concentration. Both oxygen and photosensitizer gradients are produced by microfluidic gradient-generating networks and imposed on target cells. Various photo-exposures are provided by orthogonally stacking the two layers of gray scale liquid filters. All three parameters are independently controlled in the microfluidic channels monolithically integrated in a single chip. Using this platform, we successfully demonstrated the PDT efficacy on cancer cells for different exposure doses, oxygen levels and photosensitizer concentrations.
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M3 - Conference contribution
AN - SCOPUS:84883828027
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 719
EP - 721
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -
