Highly conductive and hydrated PEG-based hydrogels for the potential application of a tissue engineering scaffold

Yong Seok Kim, Kanghee Cho, Hyun Jong Lee, Sooho Chang, Hyungsuk Lee, Jung-Hyun Kim, Won-Gun Koh

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A PEG-based hydrogel with a high conductivity and water content was synthesized using a two-step sequential polymerization process by in-situ polymerizing poly(3,4-ethylenedioxythiophene) (PEDOT) within poly(4-styrenesulfonic acid) (PSS)-containing poly(ethylene glycol) diacrylate (PEG-DA) hydrogel matrix. A small amount of diluted sulfuric acid (H2SO4) was added as an accelerator to increase the conductivity via reduced polymerization time. Among the various molecular weights (MW) of PEG, PEG-DA with MW 3400 was used for first hydrogel due to its mechanical property and water content. Incorporation of PSS within the PEG hydrogel facilitated the in-situ synthesis of PEDOT within the hydrogel, producing a hydrogel with a higher conductivity, which was further enhanced by H2SO4 treatment. The resultant semi-interpenetrating network hydrogel scaffolds were shown to consist of up to more than 80 wt% of water with a compressive modulus of 21 kPa and an electrical conductivity of 1.69 × 10− 2 S cm− 1. The surface of the resultant conductive hydrogel could be modified via photochemical fixation for cell adhesion with negligible conductivity change. In vitro studies using electro-responsive H9C2 myocytes showed that the developed hydrogels not only did not exhibit any cytotoxicity but also supported cell adhesion and proliferation. This work demonstrates that the architectural design of the conductive hydrogel scaffolds and growth mechanism of PEDOT in the hydrogel platform play a pivotal role in determining the properties of the resulting conductive hydrogel. The attractive performance of these hybrid hydrogels will open a new approach for the further research on electrically conductive tissue engineering scaffolds.

Original languageEnglish
Pages (from-to)15-22
Number of pages8
JournalReactive and Functional Polymers
Volume109
DOIs
Publication statusPublished - 2016 Dec 1

Fingerprint

Tissue Scaffolds
Hydrogels
Hydrogel
Bioelectric potentials
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Polyethylene glycols
conductivity
engineering
adhesion
polymerization
ethylene
water content
architectural design
sulfuric acid
electrical conductivity
fixation
mechanical property
Cell adhesion

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry
  • Biochemistry
  • Chemical Engineering(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

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abstract = "A PEG-based hydrogel with a high conductivity and water content was synthesized using a two-step sequential polymerization process by in-situ polymerizing poly(3,4-ethylenedioxythiophene) (PEDOT) within poly(4-styrenesulfonic acid) (PSS)-containing poly(ethylene glycol) diacrylate (PEG-DA) hydrogel matrix. A small amount of diluted sulfuric acid (H2SO4) was added as an accelerator to increase the conductivity via reduced polymerization time. Among the various molecular weights (MW) of PEG, PEG-DA with MW 3400 was used for first hydrogel due to its mechanical property and water content. Incorporation of PSS within the PEG hydrogel facilitated the in-situ synthesis of PEDOT within the hydrogel, producing a hydrogel with a higher conductivity, which was further enhanced by H2SO4 treatment. The resultant semi-interpenetrating network hydrogel scaffolds were shown to consist of up to more than 80 wt{\%} of water with a compressive modulus of 21 kPa and an electrical conductivity of 1.69 × 10− 2 S cm− 1. The surface of the resultant conductive hydrogel could be modified via photochemical fixation for cell adhesion with negligible conductivity change. In vitro studies using electro-responsive H9C2 myocytes showed that the developed hydrogels not only did not exhibit any cytotoxicity but also supported cell adhesion and proliferation. This work demonstrates that the architectural design of the conductive hydrogel scaffolds and growth mechanism of PEDOT in the hydrogel platform play a pivotal role in determining the properties of the resulting conductive hydrogel. The attractive performance of these hybrid hydrogels will open a new approach for the further research on electrically conductive tissue engineering scaffolds.",
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Highly conductive and hydrated PEG-based hydrogels for the potential application of a tissue engineering scaffold. / Kim, Yong Seok; Cho, Kanghee; Lee, Hyun Jong; Chang, Sooho; Lee, Hyungsuk; Kim, Jung-Hyun; Koh, Won-Gun.

In: Reactive and Functional Polymers, Vol. 109, 01.12.2016, p. 15-22.

Research output: Contribution to journalArticle

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AU - Kim, Yong Seok

AU - Cho, Kanghee

AU - Lee, Hyun Jong

AU - Chang, Sooho

AU - Lee, Hyungsuk

AU - Kim, Jung-Hyun

AU - Koh, Won-Gun

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