Highly efficient gene silencing and bioimaging based on fluorescent carbon dots in vitro and in vivo

Seongchan Kim, Yuri Choi, Ginam Park, Cheolhee Won, Young Joon Park, Younghoon Lee, Byeong Su Kim, Dal Hee Min

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

Small interfering RNA (siRNA) is an attractive therapeutic candidate for sequence-specific gene silencing to treat incurable diseases using small molecule drugs. However, its efficient intracellular delivery has remained a challenge. Here, we have developed a highly biocompatible fluorescent carbon dot (CD), and demonstrate a functional siRNA delivery system that induces efficient gene knockdown in vitro and in vivo. We found that CD nanoparticles (NPs) enhance the cellular uptake of siRNA, via endocytosis in tumor cells, with low cytotoxicity and unexpected immune responses. Real-time study of fluorescence imaging in live cells shows that CD NPs favorably localize in cytoplasm and successfully release siRNA within 12 h. Moreover, we demonstrate that CD NP-mediated siRNA delivery significantly silences green fluorescence protein (GFP) expression and inhibits tumor growth in a breast cancer cell xenograft mouse model of tumor-specific therapy. We have developed a multifunctional siRNA delivery vehicle enabling simultaneous bioimaging and efficient downregulation of gene expression, that shows excellent potential for gene therapy. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)503-519
Number of pages17
JournalNano Research
Volume10
Issue number2
DOIs
Publication statusPublished - 2017 Feb 1

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program (Nos. 2011-0017356 and 2011-0020322), International S&T Cooperation Program (No. 2014K1B1A1073716) and the Research Center Program (No. IBS-R008-D1) of IBS (Institute for Basic Science) through the National Research Foundation of Korea (NRF) funded by the Korean government (MEST).

Publisher Copyright:
© 2017, Tsinghua University Press and Springer-Verlag Berlin Heidelberg.

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Materials Science(all)
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

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