Highly efficient production of monoglycerides by the continuous removal of fatty acids from lipase-catalyzed oil hydrolysis

Sangpill Hwang, Sumin Lee, Ik Sung Ahn, Joon Ki Jung

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Highly efficient production of monoglycerides was achieved from lipase-catalyzed oil hydrolysis by the continuous addition of CaCl2 to remove the fatty acids produced. A fusion protein produced by connecting a cellulose-binding domain of Trichoderma hazianum cellulase to Bacillus stearothermophilus L1 lipase was used as a model 1,3-regiospecific lipase. The reaction was performed at pH 10 and 50°C, and the relationship between continuous removal of fatty acids and the production of monoglyceride was investigated by microscopic and HPLC analysis of oil emulsions and the reaction products. Without the addition of Ca2+ the reaction was inhibited by fatty acids, with the decrease in reaction rate being proportional to the concentration of fatty acids. When CaCl2 was continuously added in a 1:2 molar ratio with the released fatty acids, the reaction progressed unimpeded due to the formation of Ca-soaps. Both the yield and the fraction of monoglyceride in the reaction product increased due to the continuous removal of fatty acids.

Original languageEnglish
Pages (from-to)290-295
Number of pages6
JournalBiocatalysis and Biotransformation
Volume27
Issue number5-6
DOIs
Publication statusPublished - 2009 Nov 11

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Monoglycerides
Lipases
Lipase
Fatty acids
Hydrolysis
Oils
Fatty Acids
Reaction products
Geobacillus stearothermophilus
Soaps
Trichoderma
Soaps (detergents)
Cellulase
Bacilli
Emulsions
Cellulose
Reaction rates
Fusion reactions
High Pressure Liquid Chromatography
Proteins

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Biotechnology
  • Biochemistry

Cite this

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abstract = "Highly efficient production of monoglycerides was achieved from lipase-catalyzed oil hydrolysis by the continuous addition of CaCl2 to remove the fatty acids produced. A fusion protein produced by connecting a cellulose-binding domain of Trichoderma hazianum cellulase to Bacillus stearothermophilus L1 lipase was used as a model 1,3-regiospecific lipase. The reaction was performed at pH 10 and 50°C, and the relationship between continuous removal of fatty acids and the production of monoglyceride was investigated by microscopic and HPLC analysis of oil emulsions and the reaction products. Without the addition of Ca2+ the reaction was inhibited by fatty acids, with the decrease in reaction rate being proportional to the concentration of fatty acids. When CaCl2 was continuously added in a 1:2 molar ratio with the released fatty acids, the reaction progressed unimpeded due to the formation of Ca-soaps. Both the yield and the fraction of monoglyceride in the reaction product increased due to the continuous removal of fatty acids.",
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Highly efficient production of monoglycerides by the continuous removal of fatty acids from lipase-catalyzed oil hydrolysis. / Hwang, Sangpill; Lee, Sumin; Ahn, Ik Sung; Jung, Joon Ki.

In: Biocatalysis and Biotransformation, Vol. 27, No. 5-6, 11.11.2009, p. 290-295.

Research output: Contribution to journalArticle

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