Highly-efficient single-cell capture in microfluidic array chips using differential hydrodynamic guiding structures

Jaehoon Chung, Young Ji Kim, Euisik Yoon

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55 Citations (Scopus)

Abstract

This paper presents a highly efficient single cell capture scheme using hydrodynamic guiding structures in a microwell array. The implemented structure has a capturing efficiency of >80%, and has a capacity to place individual cells into separated microwells, allowing for the time-lapse monitoring on single cell behavior. Feasibility was tested by injecting microbeads (15 μm in diameter) and prostate cancer PC3 cells in an 8×8 microwell array chip and >80% of the microwells were occupied by single ones. Using the chips, the number of cells required for cell assays can be dramatically reduced and this will facilitate overcoming a huddle of assays with scarce supply of cells.

Original languageEnglish
Article number123701
JournalApplied Physics Letters
Volume98
Issue number12
DOIs
Publication statusPublished - 2011 Mar 21

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All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

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