Highly fluorescent conjugated polyelectrolyte for protein sensing and cell-compatible chemosensing applications

Jungmok You, Teahoon Park, Jeonghun Kim, June Seok Heo, Han Soo Kim, Hyun Ok Kim, Eunkyoung Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Using a highly fluorescent, water-soluble polymer derived from a triazine-bridged copolymer (DTMSPV), we explored the tunable fluorescence properties of the water-soluble DTMSPV by solvent polarity to function as a fluorescence sensory probe for protein sensing. The green-blue fluorescence from DTMSPV was significantly enhanced in the presence of bovine serum albumin through hydrophobic interactions. Meanwhile, complete quenching of the fluorescence from DTMSPV occurred in the presence of hemoglobin through iron complexation with the polyelectrolyte. In addition, the DTMSPVs were highly fluorescent and permeated into living mesenchymal stem cells (MSCs), enabling effective imaging of the MSCs. This permeation into stem cells is crucial to the detection of Al3+ in living MSCs. The interaction between the triazine units in DTMSPV with the Al3+ ions allows for the detection of Al3+ in living cells. Thus, a strong fluorescence from living MSCs pretreated with DTMSPV was quenched as a function of the Al3+ concentration, confirming that DTMSPV is a cell-permeable fluorescent polymer that can function as a versatile probe to detect Al3+ in living cells.

Original languageEnglish
Pages (from-to)3305-3311
Number of pages7
JournalACS Applied Materials and Interfaces
Volume6
Issue number5
DOIs
Publication statusPublished - 2014 Mar 12

Fingerprint

Stem cells
Polyelectrolytes
Fluorescence
Proteins
Triazines
Polymers
Cells
Water
Hemoglobin
Bovine Serum Albumin
Complexation
Permeation
Quenching
Hemoglobins
Iron
Copolymers
Ions
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

You, Jungmok ; Park, Teahoon ; Kim, Jeonghun ; Heo, June Seok ; Kim, Han Soo ; Kim, Hyun Ok ; Kim, Eunkyoung. / Highly fluorescent conjugated polyelectrolyte for protein sensing and cell-compatible chemosensing applications. In: ACS Applied Materials and Interfaces. 2014 ; Vol. 6, No. 5. pp. 3305-3311.
@article{792067a7fead4887b0fe0217f3b4f093,
title = "Highly fluorescent conjugated polyelectrolyte for protein sensing and cell-compatible chemosensing applications",
abstract = "Using a highly fluorescent, water-soluble polymer derived from a triazine-bridged copolymer (DTMSPV), we explored the tunable fluorescence properties of the water-soluble DTMSPV by solvent polarity to function as a fluorescence sensory probe for protein sensing. The green-blue fluorescence from DTMSPV was significantly enhanced in the presence of bovine serum albumin through hydrophobic interactions. Meanwhile, complete quenching of the fluorescence from DTMSPV occurred in the presence of hemoglobin through iron complexation with the polyelectrolyte. In addition, the DTMSPVs were highly fluorescent and permeated into living mesenchymal stem cells (MSCs), enabling effective imaging of the MSCs. This permeation into stem cells is crucial to the detection of Al3+ in living MSCs. The interaction between the triazine units in DTMSPV with the Al3+ ions allows for the detection of Al3+ in living cells. Thus, a strong fluorescence from living MSCs pretreated with DTMSPV was quenched as a function of the Al3+ concentration, confirming that DTMSPV is a cell-permeable fluorescent polymer that can function as a versatile probe to detect Al3+ in living cells.",
author = "Jungmok You and Teahoon Park and Jeonghun Kim and Heo, {June Seok} and Kim, {Han Soo} and Kim, {Hyun Ok} and Eunkyoung Kim",
year = "2014",
month = "3",
day = "12",
doi = "10.1021/am500269t",
language = "English",
volume = "6",
pages = "3305--3311",
journal = "ACS applied materials & interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "5",

}

Highly fluorescent conjugated polyelectrolyte for protein sensing and cell-compatible chemosensing applications. / You, Jungmok; Park, Teahoon; Kim, Jeonghun; Heo, June Seok; Kim, Han Soo; Kim, Hyun Ok; Kim, Eunkyoung.

In: ACS Applied Materials and Interfaces, Vol. 6, No. 5, 12.03.2014, p. 3305-3311.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Highly fluorescent conjugated polyelectrolyte for protein sensing and cell-compatible chemosensing applications

AU - You, Jungmok

AU - Park, Teahoon

AU - Kim, Jeonghun

AU - Heo, June Seok

AU - Kim, Han Soo

AU - Kim, Hyun Ok

AU - Kim, Eunkyoung

PY - 2014/3/12

Y1 - 2014/3/12

N2 - Using a highly fluorescent, water-soluble polymer derived from a triazine-bridged copolymer (DTMSPV), we explored the tunable fluorescence properties of the water-soluble DTMSPV by solvent polarity to function as a fluorescence sensory probe for protein sensing. The green-blue fluorescence from DTMSPV was significantly enhanced in the presence of bovine serum albumin through hydrophobic interactions. Meanwhile, complete quenching of the fluorescence from DTMSPV occurred in the presence of hemoglobin through iron complexation with the polyelectrolyte. In addition, the DTMSPVs were highly fluorescent and permeated into living mesenchymal stem cells (MSCs), enabling effective imaging of the MSCs. This permeation into stem cells is crucial to the detection of Al3+ in living MSCs. The interaction between the triazine units in DTMSPV with the Al3+ ions allows for the detection of Al3+ in living cells. Thus, a strong fluorescence from living MSCs pretreated with DTMSPV was quenched as a function of the Al3+ concentration, confirming that DTMSPV is a cell-permeable fluorescent polymer that can function as a versatile probe to detect Al3+ in living cells.

AB - Using a highly fluorescent, water-soluble polymer derived from a triazine-bridged copolymer (DTMSPV), we explored the tunable fluorescence properties of the water-soluble DTMSPV by solvent polarity to function as a fluorescence sensory probe for protein sensing. The green-blue fluorescence from DTMSPV was significantly enhanced in the presence of bovine serum albumin through hydrophobic interactions. Meanwhile, complete quenching of the fluorescence from DTMSPV occurred in the presence of hemoglobin through iron complexation with the polyelectrolyte. In addition, the DTMSPVs were highly fluorescent and permeated into living mesenchymal stem cells (MSCs), enabling effective imaging of the MSCs. This permeation into stem cells is crucial to the detection of Al3+ in living MSCs. The interaction between the triazine units in DTMSPV with the Al3+ ions allows for the detection of Al3+ in living cells. Thus, a strong fluorescence from living MSCs pretreated with DTMSPV was quenched as a function of the Al3+ concentration, confirming that DTMSPV is a cell-permeable fluorescent polymer that can function as a versatile probe to detect Al3+ in living cells.

UR - http://www.scopus.com/inward/record.url?scp=84896332320&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896332320&partnerID=8YFLogxK

U2 - 10.1021/am500269t

DO - 10.1021/am500269t

M3 - Article

C2 - 24527754

AN - SCOPUS:84896332320

VL - 6

SP - 3305

EP - 3311

JO - ACS applied materials & interfaces

JF - ACS applied materials & interfaces

SN - 1944-8244

IS - 5

ER -