Highly sensitive and selective spectroscopic detection of mercury(II) in water by using pyridylporphyrin-DNA conjugates

Jung Kyu Choi, Gevorg Sargsyan, Amanda M. Olive, Milan Balaz

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Single-labeled pyridylporphyrin-DNA conjugates are reported as highly sensitive and selective spectroscopic sensors for mercury(II) ions in water. The effects of chemical structure (thymine versus adenine), number of nucleotides (monomer versus octamer), and porphyrin metalation (Zn versus free base) on the sensitivity and selectivity of mercury(II) detection are explored. The results indicated that pyridylporphyrin rather than the nucleobase plays a crucial role in mercury(II) sensing, because porphyrin conjugates with both adenosine and thymidine exhibited excellent mercury(II) detection. Mercury(II) recognition was shown in emission quenching, as well as in a redshift of the porphyrin Soret band absorption. The limit of detection (LOD, 3σ/slope) of zinc(II) pyridylporphyrin-5′-oligodeoxythymidine (ZnPorT8) obtained by fluorescence quenching was calculated to be 21.14 nM. Other metal cations (Zn2+, Cd2+, Pb2+, Mn2+, Ca2+, Ni 2+, Mg2+, Fe2+, Cu2+, and Na +) did not interfere with the emission and absorption sensing of mercury(II). Free-base porphyrin-oligothymine conjugate 2HPorT8 displayed similar sensitivity to ZnPorT8 but different selectivity. The results also implied that the sensing properties of porphyrin-deoxythymidine conjugates could potentially be tuned by porphyrin metalation. Dark H(u)g: Mercury(II) quenches the emission of pyridylporphyrin-DNA conjugates (see figure), which allows its detection in water in the presence of other heavy metals. The effect of chemical structure, number of nucleotides, and porphyrin metalation on the sensitivity and selectivity of detection is explored. The results indicate that porphyrin, rather than thymine, plays a crucial role in mercury(II) sensing.

Original languageEnglish
Pages (from-to)2515-2522
Number of pages8
JournalChemistry - A European Journal
Volume19
Issue number7
DOIs
Publication statusPublished - 2013 Feb 11

Fingerprint

Porphyrins
Mercury
DNA
Water
Thymine
Nucleotides
Thymidine
Quenching
Adenine
Heavy Metals
Adenosine
Heavy metals
Cations
Zinc
Absorption spectra
Monomers
Positive ions
Metals
Fluorescence
Ions

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

@article{6d4c4b1b220246d7a66a10f110d82c4c,
title = "Highly sensitive and selective spectroscopic detection of mercury(II) in water by using pyridylporphyrin-DNA conjugates",
abstract = "Single-labeled pyridylporphyrin-DNA conjugates are reported as highly sensitive and selective spectroscopic sensors for mercury(II) ions in water. The effects of chemical structure (thymine versus adenine), number of nucleotides (monomer versus octamer), and porphyrin metalation (Zn versus free base) on the sensitivity and selectivity of mercury(II) detection are explored. The results indicated that pyridylporphyrin rather than the nucleobase plays a crucial role in mercury(II) sensing, because porphyrin conjugates with both adenosine and thymidine exhibited excellent mercury(II) detection. Mercury(II) recognition was shown in emission quenching, as well as in a redshift of the porphyrin Soret band absorption. The limit of detection (LOD, 3σ/slope) of zinc(II) pyridylporphyrin-5′-oligodeoxythymidine (ZnPorT8) obtained by fluorescence quenching was calculated to be 21.14 nM. Other metal cations (Zn2+, Cd2+, Pb2+, Mn2+, Ca2+, Ni 2+, Mg2+, Fe2+, Cu2+, and Na +) did not interfere with the emission and absorption sensing of mercury(II). Free-base porphyrin-oligothymine conjugate 2HPorT8 displayed similar sensitivity to ZnPorT8 but different selectivity. The results also implied that the sensing properties of porphyrin-deoxythymidine conjugates could potentially be tuned by porphyrin metalation. Dark H(u)g: Mercury(II) quenches the emission of pyridylporphyrin-DNA conjugates (see figure), which allows its detection in water in the presence of other heavy metals. The effect of chemical structure, number of nucleotides, and porphyrin metalation on the sensitivity and selectivity of detection is explored. The results indicate that porphyrin, rather than thymine, plays a crucial role in mercury(II) sensing.",
author = "Choi, {Jung Kyu} and Gevorg Sargsyan and Olive, {Amanda M.} and Milan Balaz",
year = "2013",
month = "2",
day = "11",
doi = "10.1002/chem.201202461",
language = "English",
volume = "19",
pages = "2515--2522",
journal = "Chemistry - A European Journal",
issn = "0947-6539",
publisher = "Wiley-VCH Verlag",
number = "7",

}

Highly sensitive and selective spectroscopic detection of mercury(II) in water by using pyridylporphyrin-DNA conjugates. / Choi, Jung Kyu; Sargsyan, Gevorg; Olive, Amanda M.; Balaz, Milan.

In: Chemistry - A European Journal, Vol. 19, No. 7, 11.02.2013, p. 2515-2522.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Highly sensitive and selective spectroscopic detection of mercury(II) in water by using pyridylporphyrin-DNA conjugates

AU - Choi, Jung Kyu

AU - Sargsyan, Gevorg

AU - Olive, Amanda M.

AU - Balaz, Milan

PY - 2013/2/11

Y1 - 2013/2/11

N2 - Single-labeled pyridylporphyrin-DNA conjugates are reported as highly sensitive and selective spectroscopic sensors for mercury(II) ions in water. The effects of chemical structure (thymine versus adenine), number of nucleotides (monomer versus octamer), and porphyrin metalation (Zn versus free base) on the sensitivity and selectivity of mercury(II) detection are explored. The results indicated that pyridylporphyrin rather than the nucleobase plays a crucial role in mercury(II) sensing, because porphyrin conjugates with both adenosine and thymidine exhibited excellent mercury(II) detection. Mercury(II) recognition was shown in emission quenching, as well as in a redshift of the porphyrin Soret band absorption. The limit of detection (LOD, 3σ/slope) of zinc(II) pyridylporphyrin-5′-oligodeoxythymidine (ZnPorT8) obtained by fluorescence quenching was calculated to be 21.14 nM. Other metal cations (Zn2+, Cd2+, Pb2+, Mn2+, Ca2+, Ni 2+, Mg2+, Fe2+, Cu2+, and Na +) did not interfere with the emission and absorption sensing of mercury(II). Free-base porphyrin-oligothymine conjugate 2HPorT8 displayed similar sensitivity to ZnPorT8 but different selectivity. The results also implied that the sensing properties of porphyrin-deoxythymidine conjugates could potentially be tuned by porphyrin metalation. Dark H(u)g: Mercury(II) quenches the emission of pyridylporphyrin-DNA conjugates (see figure), which allows its detection in water in the presence of other heavy metals. The effect of chemical structure, number of nucleotides, and porphyrin metalation on the sensitivity and selectivity of detection is explored. The results indicate that porphyrin, rather than thymine, plays a crucial role in mercury(II) sensing.

AB - Single-labeled pyridylporphyrin-DNA conjugates are reported as highly sensitive and selective spectroscopic sensors for mercury(II) ions in water. The effects of chemical structure (thymine versus adenine), number of nucleotides (monomer versus octamer), and porphyrin metalation (Zn versus free base) on the sensitivity and selectivity of mercury(II) detection are explored. The results indicated that pyridylporphyrin rather than the nucleobase plays a crucial role in mercury(II) sensing, because porphyrin conjugates with both adenosine and thymidine exhibited excellent mercury(II) detection. Mercury(II) recognition was shown in emission quenching, as well as in a redshift of the porphyrin Soret band absorption. The limit of detection (LOD, 3σ/slope) of zinc(II) pyridylporphyrin-5′-oligodeoxythymidine (ZnPorT8) obtained by fluorescence quenching was calculated to be 21.14 nM. Other metal cations (Zn2+, Cd2+, Pb2+, Mn2+, Ca2+, Ni 2+, Mg2+, Fe2+, Cu2+, and Na +) did not interfere with the emission and absorption sensing of mercury(II). Free-base porphyrin-oligothymine conjugate 2HPorT8 displayed similar sensitivity to ZnPorT8 but different selectivity. The results also implied that the sensing properties of porphyrin-deoxythymidine conjugates could potentially be tuned by porphyrin metalation. Dark H(u)g: Mercury(II) quenches the emission of pyridylporphyrin-DNA conjugates (see figure), which allows its detection in water in the presence of other heavy metals. The effect of chemical structure, number of nucleotides, and porphyrin metalation on the sensitivity and selectivity of detection is explored. The results indicate that porphyrin, rather than thymine, plays a crucial role in mercury(II) sensing.

UR - http://www.scopus.com/inward/record.url?scp=84873354254&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84873354254&partnerID=8YFLogxK

U2 - 10.1002/chem.201202461

DO - 10.1002/chem.201202461

M3 - Article

C2 - 23239513

AN - SCOPUS:84873354254

VL - 19

SP - 2515

EP - 2522

JO - Chemistry - A European Journal

JF - Chemistry - A European Journal

SN - 0947-6539

IS - 7

ER -