Highly sensitive bacterial susceptibility test against penicillin using parylene-matrix chip

Jong Min Park, Jo Il Kim, Hyun Woo Song, Joo Yoon Noh, Min Jung Kang, Jae Chul Pyun

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This work presented a highly sensitive bacterial antibiotic susceptibility test through β-lactamase assay using Parylene-matrix chip. β-lactamases (EC 3.5.2.6) are an important family of enzymes that confer resistance to β-lactam antibiotics by catalyzing the hydrolysis of these antibiotics. Here we present a highly sensitive assay to quantitate β-lactamase-mediated hydrolysis of penicillin into penicilloic acid. Typically, MALDI-TOF mass spectrometry has been used to quantitate low molecular weight analytes and to discriminate them from noise peaks of matrix fragments that occur at low m/z ratios (m/z<500). The β-lactamase assay for the Escherichia coli antibiotic susceptibility test was carried out using Parylene-matrix chip and MALDI-TOF mass spectrometry. The Parylene-matrix chip was successfully used to quantitate penicillin (m/z: [PEN+H]+=335.1 and [PEN+Na]+=357.8) and penicilloic acid (m/z: [PA+H]+=353.1) in a β-lactamase assay with minimal interference of low molecular weight noise peaks. The β-lactamase assay was carried out with an antibiotic-resistant E. coli strain and an antibiotic-susceptible E. coli strain, revealing that the minimum number of E. coli cells required to screen for antibiotic resistance was 1000 cells for the MALDI-TOF mass spectrometry/Parylene-matrix chip assay.

Original languageEnglish
Pages (from-to)306-312
Number of pages7
JournalBiosensors and Bioelectronics
Volume71
DOIs
Publication statusPublished - 2015 Sep 5

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Antibiotics
Penicillins
Assays
Anti-Bacterial Agents
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Escherichia coli
Mass Spectrometry
Mass spectrometry
Noise
Hydrolysis
Molecular Weight
Molecular weight
Lactams
Microbial Drug Resistance
Acids
parylene
Enzymes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Park, Jong Min ; Kim, Jo Il ; Song, Hyun Woo ; Noh, Joo Yoon ; Kang, Min Jung ; Pyun, Jae Chul. / Highly sensitive bacterial susceptibility test against penicillin using parylene-matrix chip. In: Biosensors and Bioelectronics. 2015 ; Vol. 71. pp. 306-312.
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Highly sensitive bacterial susceptibility test against penicillin using parylene-matrix chip. / Park, Jong Min; Kim, Jo Il; Song, Hyun Woo; Noh, Joo Yoon; Kang, Min Jung; Pyun, Jae Chul.

In: Biosensors and Bioelectronics, Vol. 71, 05.09.2015, p. 306-312.

Research output: Contribution to journalArticle

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