Highly Sensitive Determination of Concanavalin A Lectin Based on Silver-Enhanced Electrogenerated Chemiluminescence of Luminol

Jinju Kwon, Kwang Soo Ahn, Daeho Jeong, Han Nim Choi, Wonyong Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A highly sensitive bioassay based on silver-enhanced luminol electrogenerated chemiluminescence (ECL) is reported for the determination of concanavalin A lectin. A gold electrode modified with the mixed self-assembled monolayer of thiolated mannoside and mercaptohexanol was used to selectively capture a target lectin, concanavalin A, through the specific interaction between mannoside and concanavalin A. Mannoside-functionalized gold nanoparticles were further introduced to the opposite binding sites of the tetrameric concanavalin A to form a sandwich-type complex. Silver enhancement step was performed to coat the surface of mannose-stabilized gold nanoparticles with silver. The deposited silver was dissolved in an acidic solution and further neutralized. The resulting silver ions were finally detected with luminol electrogenerated chemiluminescence, in which the silver ions greatly enhanced the chemiluminescence intensity. The present electrogenerated chemiluminescence bioassay detected concanavalin A from 0.190 to 10.0 µg/mL (r2 = 0.999) with a detection limit of 0.146 µg/mL (signal to noise ratio = 3), which is much lower compared to previously reported methods such as microgravimetry, surface plasmon resonance, and colorimetry. Furthermore, the present bioassay showed good selectivity over possible interfering lectin proteins.

Original languageEnglish
Pages (from-to)2114-2127
Number of pages14
JournalAnalytical Letters
Volume51
Issue number13
DOIs
Publication statusPublished - 2018 Sep 2

Fingerprint

Luminol
Chemiluminescence
Concanavalin A
Luminescence
Silver
Lectins
Mannosides
Bioassay
Gold
Biological Assay
Nanoparticles
Concanavalin A Receptors
Ions
Colorimetry
Surface Plasmon Resonance
Surface plasmon resonance
Signal-To-Noise Ratio
Self assembled monolayers
Binding sites
Mannose

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

Cite this

Kwon, Jinju ; Ahn, Kwang Soo ; Jeong, Daeho ; Choi, Han Nim ; Lee, Wonyong. / Highly Sensitive Determination of Concanavalin A Lectin Based on Silver-Enhanced Electrogenerated Chemiluminescence of Luminol. In: Analytical Letters. 2018 ; Vol. 51, No. 13. pp. 2114-2127.
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Highly Sensitive Determination of Concanavalin A Lectin Based on Silver-Enhanced Electrogenerated Chemiluminescence of Luminol. / Kwon, Jinju; Ahn, Kwang Soo; Jeong, Daeho; Choi, Han Nim; Lee, Wonyong.

In: Analytical Letters, Vol. 51, No. 13, 02.09.2018, p. 2114-2127.

Research output: Contribution to journalArticle

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