Highly sensitive immunoassay for the diagnosis of acute myocardial infarction using silica spheres encapsulating a quantum dot layer

Hyojeong Han, Jae Chul Pyun, Hyein Yoo, Hong Seog Seo, Byung Hwa Jung, Young Sook Yoo, Kyoungja Woo, Min Jung Kang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Commercial ELISA kits for substance P (SubP), which are helpful for the clinical diagnosis of acute myocardial infarction, are limited in efficacy because of low sensitivity. A highly sensitive immunoassay was developed using silica spheres encapsulating a quantum dot-layer (SQS) and labeling antibodies, on a Parylene A-modified plate. The high sensitivity was possible by taking advantage of the enhanced photoluminescence of the SQS and dense immobilization of SubP on a Parylene A-modified plate. Glutaraldehyde was used for cross-linking of SQS to the anti-SubP antibody and SubP to the Parylene A coating. The SQS-linked immunosorbent assay (SQSLISA) was optimized and validated. The dynamic range for the assay was 1-10000 pg/mL with a linear correlation factor of 0.9992 when the competitive SQSLISA was employed. The intra- and interday accuracies were 93-100% and 87-122%, respectively. The reproducibility was lower than 11%. The developed method was applied to clinical samples collected from healthy controls (n = 30) and acute myocardial infarction (n = 16) and it displayed a high correlation with the commercial ELISA kit, with a limit of detection that was 30-fold lower. Clinical sample analysis confirmed that SubP is a promising diagnostic marker for acute myocardial infarction. The SQSLISA is expected to be a practical and useful assay tool.

Original languageEnglish
Pages (from-to)10157-10163
Number of pages7
JournalAnalytical Chemistry
Volume86
Issue number20
DOIs
Publication statusPublished - 2014 Oct 21

Fingerprint

Substance P
Silicon Dioxide
Semiconductor quantum dots
Assays
Immunosorbents
Antibodies
Glutaral
Labeling
Photoluminescence
Coatings
parylene

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Han, Hyojeong ; Pyun, Jae Chul ; Yoo, Hyein ; Seo, Hong Seog ; Jung, Byung Hwa ; Yoo, Young Sook ; Woo, Kyoungja ; Kang, Min Jung. / Highly sensitive immunoassay for the diagnosis of acute myocardial infarction using silica spheres encapsulating a quantum dot layer. In: Analytical Chemistry. 2014 ; Vol. 86, No. 20. pp. 10157-10163.
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Highly sensitive immunoassay for the diagnosis of acute myocardial infarction using silica spheres encapsulating a quantum dot layer. / Han, Hyojeong; Pyun, Jae Chul; Yoo, Hyein; Seo, Hong Seog; Jung, Byung Hwa; Yoo, Young Sook; Woo, Kyoungja; Kang, Min Jung.

In: Analytical Chemistry, Vol. 86, No. 20, 21.10.2014, p. 10157-10163.

Research output: Contribution to journalArticle

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AU - Han, Hyojeong

AU - Pyun, Jae Chul

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AU - Kang, Min Jung

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