Highly sensitive metal-enhanced fluorescence biosensor prepared on electrospun fibers decorated with silica-coated silver nanoparticles

Byung Ju Yun, Ji Eon Kwon, Kangwon Lee, Won Gun Koh

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)


A new metal-enhanced fluorescence (MEF)-based biosensor platform was prepared on fibrous substrates. First, polycaprolactone (PCL) fibers were obtained via electrospinning process. PCL fibers were decorated with photoreduced silver nanoparticles followed by silica coating, generating silver-decorated PCL fibers with a silica layer (Ag@SiO2-PCL). The silica layer acted as a spacer between the silver nanoparticles and the fluorescent molecules to optimize the MEF effect. The MEF was obtained from the silver-decorated PCL fibers, the extent of which could be controlled by the thickness of the silica layer. The fibrous structure of Ag@SiO2-PCL had higher protein loading capacity than conventional two-dimensional glass slides due to its large surface area, while the presence of silica-coated silver nanoparticles resulted in higher fluorescence intensity than silica-coated PCL fibers (SiO2-PCL) without silver nanoparticles via the MEF effect. Both features of Ag@SiO2-PCL were combined to produce combined effects in improving the performance of fluorescence-based biosensing. According to immunobinding assays between fluorescently labeled anti-IgG and IgG immobilized onto different substrates, Ag@SiO2-PCL had much better sensing performance in terms of sensitivity and detection limit than glass slides and SiO2-PCL due to the combined effects of a large surface area and the MEF effect.

Original languageEnglish
Pages (from-to)140-147
Number of pages8
JournalSensors and Actuators, B: Chemical
Publication statusPublished - 2019 Apr 1

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation (NRF) grant funded by the Ministry of Science and ICT (MSIT) ( NRF-2017M3A7B4049848 , NRF-2017M3D1A1039289 and 2017M3A7B4041798 )

Publisher Copyright:
© 2018 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


Dive into the research topics of 'Highly sensitive metal-enhanced fluorescence biosensor prepared on electrospun fibers decorated with silica-coated silver nanoparticles'. Together they form a unique fingerprint.

Cite this