Highly-sensitive single-step sensing of levodopa by swellable microneedle-mounted nanogap sensors

Seung Hyun Park; Yong Jae Kim; Elisabeth Kostal; Volha Matylitskaya; Stefan Partel; Won Hyoung Ryu

doi:10.1016/j.bios.2022.114912

Highly-sensitive single-step sensing of levodopa by swellable microneedle-mounted nanogap sensors

Seung Hyun Park, Yong Jae Kim, Elisabeth Kostal, Volha Matylitskaya, Stefan Partel, Won Hyoung Ryu

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Microneedle (MN) sensing of biomarkers in interstitial fluid (ISF) can overcome the challenges of self-diagnosis of diseases by a patient, such as blood sampling, handling, and measurement analysis. However, the MN sensing technologies still suffer from poor measurement accuracy due to the small amount of target molecules present in ISF, and require multiple steps of ISF extraction, ISF isolation from MN, and measurement with additional equipment. Here, we present a swellable MN-mounted nanogap sensor that can be inserted into the skin tissue, absorb ISF rapidly, and measure biomarkers in situ by amplifying the measurement signals by redox cycling in nanogap electrodes. We demonstrate that the MN-nanogap sensor measures levodopa (LDA), medication for Parkinson disease, down to 100 nM in an aqueous solution, and 1 μM in both the skin-mimicked gelatin phantom and porcine skin.

Original language	English
Article number	114912
Journal	Biosensors and Bioelectronics
Volume	220
DOIs	https://doi.org/10.1016/j.bios.2022.114912
Publication status	Published - 2023 Jan 15

Bibliographical note

Funding Information:
This work was supported by Global Infrastructure Program through the National Research Foundation of Korea (NRF) funded by the MSIT ( 2019K1A3A1A18116048 ), South Korea(W.R.), the National Research Foundation of Korea (NRF) grant funded by the Korea Government(MSIT) ( 2020R1A2C3013158 ), South Korea(W.R.), the Yonsei-KIST Convergence Research Program, South Korea(W.R.), and the Austrian Agency of Education and Internationalisation (OeAD) ( KR 05/2020 ), Austria(S.P.).

Publisher Copyright:
© 2022 Elsevier B.V.

All Science Journal Classification (ASJC) codes

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2022.114912

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title = "Highly-sensitive single-step sensing of levodopa by swellable microneedle-mounted nanogap sensors",

abstract = "Microneedle (MN) sensing of biomarkers in interstitial fluid (ISF) can overcome the challenges of self-diagnosis of diseases by a patient, such as blood sampling, handling, and measurement analysis. However, the MN sensing technologies still suffer from poor measurement accuracy due to the small amount of target molecules present in ISF, and require multiple steps of ISF extraction, ISF isolation from MN, and measurement with additional equipment. Here, we present a swellable MN-mounted nanogap sensor that can be inserted into the skin tissue, absorb ISF rapidly, and measure biomarkers in situ by amplifying the measurement signals by redox cycling in nanogap electrodes. We demonstrate that the MN-nanogap sensor measures levodopa (LDA), medication for Parkinson disease, down to 100 nM in an aqueous solution, and 1 μM in both the skin-mimicked gelatin phantom and porcine skin.",

author = "Park, {Seung Hyun} and Kim, {Yong Jae} and Elisabeth Kostal and Volha Matylitskaya and Stefan Partel and Ryu, {Won Hyoung}",

note = "Funding Information: This work was supported by Global Infrastructure Program through the National Research Foundation of Korea (NRF) funded by the MSIT ( 2019K1A3A1A18116048 ), South Korea(W.R.), the National Research Foundation of Korea (NRF) grant funded by the Korea Government(MSIT) ( 2020R1A2C3013158 ), South Korea(W.R.), the Yonsei-KIST Convergence Research Program, South Korea(W.R.), and the Austrian Agency of Education and Internationalisation (OeAD) ( KR 05/2020 ), Austria(S.P.). Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

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AU - Matylitskaya, Volha

AU - Partel, Stefan

AU - Ryu, Won Hyoung

N1 - Funding Information: This work was supported by Global Infrastructure Program through the National Research Foundation of Korea (NRF) funded by the MSIT ( 2019K1A3A1A18116048 ), South Korea(W.R.), the National Research Foundation of Korea (NRF) grant funded by the Korea Government(MSIT) ( 2020R1A2C3013158 ), South Korea(W.R.), the Yonsei-KIST Convergence Research Program, South Korea(W.R.), and the Austrian Agency of Education and Internationalisation (OeAD) ( KR 05/2020 ), Austria(S.P.). Publisher Copyright: © 2022 Elsevier B.V.

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N2 - Microneedle (MN) sensing of biomarkers in interstitial fluid (ISF) can overcome the challenges of self-diagnosis of diseases by a patient, such as blood sampling, handling, and measurement analysis. However, the MN sensing technologies still suffer from poor measurement accuracy due to the small amount of target molecules present in ISF, and require multiple steps of ISF extraction, ISF isolation from MN, and measurement with additional equipment. Here, we present a swellable MN-mounted nanogap sensor that can be inserted into the skin tissue, absorb ISF rapidly, and measure biomarkers in situ by amplifying the measurement signals by redox cycling in nanogap electrodes. We demonstrate that the MN-nanogap sensor measures levodopa (LDA), medication for Parkinson disease, down to 100 nM in an aqueous solution, and 1 μM in both the skin-mimicked gelatin phantom and porcine skin.

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Highly-sensitive single-step sensing of levodopa by swellable microneedle-mounted nanogap sensors

Abstract

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