Highly tunable aptasensing microarrays with graphene oxide multilayers

Yun Kyung Jung, Taemin Lee, Eeseul Shin, Byeong Su Kim

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)


A highly tunable layer-by-layer (LbL)-assembled graphene oxide (GO) array has been devised for high-throughput multiplex protein sensing. In this array, the fluorescence of different target-bound aptamers labeled with dye is efficiently quenched by GO through fluorescence resonance energy transfer (FRET), and simultaneous multiplex target detection is performed by recovering the quenched fluorescence caused by specific binding between an aptamer and a protein. Thin GO films consisting of 10 bilayers displayed a high quenching ability, yielding over 85% fluorescence quenching with the addition of a 2â€...μM dye-labeled aptamer. The limit for human thrombin detection in the 6- and 10-bilayered GO array is estimated to be 0.1 and 0.001â€...nM, respectively, indicating highly tunable nature of LbL assembled GO multilayers in controlling the sensitivity of graphene-based FRET aptasensor. Furthermore, the GO chip could be reused up to four times simply by cleaning it with distilled water.

Original languageEnglish
Article number3367
JournalScientific reports
Publication statusPublished - 2013 Nov 28

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2012R1A1A3015259) and by the grant funded by the Korean Government (No. 2010-0028684).

All Science Journal Classification (ASJC) codes

  • General


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