A highly coordinated complex known as the microprocessor precisely processes primary transcripts of MIRNA genes into mature miRNAs. In plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE). To precisely modulate miRNA maturation, the microprocessor cooperates with at least 12 proteins in plants. In addition, we here show the involvement of a novel gene, HYL1-interacting GIY-YIG-like endonuclease (HIGLE). The encoded protein has a GIY-YIG domain that is generally found within a class of homing endonucleases. HIGLE directly interacts with the microprocessor components HYL1 and SE. Unlike the functions of other GIY-YIG endonucleases, the catalytic core of HIGLE has both DNase and RNase activities that sufficiently processes miRNA precursors into short fragments in vitro.
Bibliographical noteFunding Information:
This work was supported by grants from the National Center for Next-Generation BioGreen21 Program (PJ01194601), the National Research Foundation (NRF-2016R1C1B1013506 and NRF-2016K2A9A1A06922119), Yonsei University Research Fund (2016-12-0006), and ?Center of synthetic biology? funded by the UNIK Research Initiative of the Danish Ministry of Science, Technology and Innovation.
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology
- Cell Biology