Abstract
A highly coordinated complex known as the microprocessor precisely processes primary transcripts of MIRNA genes into mature miRNAs. In plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE). To precisely modulate miRNA maturation, the microprocessor cooperates with at least 12 proteins in plants. In addition, we here show the involvement of a novel gene, HYL1-interacting GIY-YIG-like endonuclease (HIGLE). The encoded protein has a GIY-YIG domain that is generally found within a class of homing endonucleases. HIGLE directly interacts with the microprocessor components HYL1 and SE. Unlike the functions of other GIY-YIG endonucleases, the catalytic core of HIGLE has both DNase and RNase activities that sufficiently processes miRNA precursors into short fragments in vitro.
| Original language | English |
|---|---|
| Pages (from-to) | 1383-1393 |
| Number of pages | 11 |
| Journal | FEBS Letters |
| Volume | 591 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2017 May 1 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
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HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana. / Cho, Seok Keun; Ryu, Moon Young; Poulsen, Christian; Kim, Jong Hum; Oh, Tae Rin; Choi, Suk Won; Kim, Mijung; Yang, Jun Yi; Boo, Kyung Hwan; Geshi, Naomi; Kim, Woo Taek; Yang, Seong Wook.
In: FEBS Letters, Vol. 591, No. 10, 01.05.2017, p. 1383-1393.Research output: Contribution to journal › Letter
TY - JOUR
T1 - HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana
AU - Cho, Seok Keun
AU - Ryu, Moon Young
AU - Poulsen, Christian
AU - Kim, Jong Hum
AU - Oh, Tae Rin
AU - Choi, Suk Won
AU - Kim, Mijung
AU - Yang, Jun Yi
AU - Boo, Kyung Hwan
AU - Geshi, Naomi
AU - Kim, Woo Taek
AU - Yang, Seong Wook
PY - 2017/5/1
Y1 - 2017/5/1
N2 - A highly coordinated complex known as the microprocessor precisely processes primary transcripts of MIRNA genes into mature miRNAs. In plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE). To precisely modulate miRNA maturation, the microprocessor cooperates with at least 12 proteins in plants. In addition, we here show the involvement of a novel gene, HYL1-interacting GIY-YIG-like endonuclease (HIGLE). The encoded protein has a GIY-YIG domain that is generally found within a class of homing endonucleases. HIGLE directly interacts with the microprocessor components HYL1 and SE. Unlike the functions of other GIY-YIG endonucleases, the catalytic core of HIGLE has both DNase and RNase activities that sufficiently processes miRNA precursors into short fragments in vitro.
AB - A highly coordinated complex known as the microprocessor precisely processes primary transcripts of MIRNA genes into mature miRNAs. In plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE). To precisely modulate miRNA maturation, the microprocessor cooperates with at least 12 proteins in plants. In addition, we here show the involvement of a novel gene, HYL1-interacting GIY-YIG-like endonuclease (HIGLE). The encoded protein has a GIY-YIG domain that is generally found within a class of homing endonucleases. HIGLE directly interacts with the microprocessor components HYL1 and SE. Unlike the functions of other GIY-YIG endonucleases, the catalytic core of HIGLE has both DNase and RNase activities that sufficiently processes miRNA precursors into short fragments in vitro.
UR - http://www.scopus.com/inward/record.url?scp=85018372906&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85018372906&partnerID=8YFLogxK
U2 - 10.1002/1873-3468.12628
DO - 10.1002/1873-3468.12628
M3 - Letter
C2 - 28321834
AN - SCOPUS:85018372906
VL - 591
SP - 1383
EP - 1393
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 10
ER -