HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana

Seok Keun Cho; Moon Young Ryu; Christian Poulsen; Jong Hum Kim; Tae Rin Oh; Suk Won Choi; Mijung Kim; Jun Yi Yang; Kyung Hwan Boo; Naomi Geshi; Woo Taek Kim; Seong Wook Yang

doi:10.1002/1873-3468.12628

HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana

Seok Keun Cho, Moon Young Ryu, Christian Poulsen, Jong Hum Kim, Tae Rin Oh, Suk Won Choi, Mijung Kim, Jun Yi Yang, Kyung Hwan Boo, Naomi Geshi, Woo Taek Kim, Seong Wook Yang

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A highly coordinated complex known as the microprocessor precisely processes primary transcripts of MIRNA genes into mature miRNAs. In plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE). To precisely modulate miRNA maturation, the microprocessor cooperates with at least 12 proteins in plants. In addition, we here show the involvement of a novel gene, HYL1-interacting GIY-YIG-like endonuclease (HIGLE). The encoded protein has a GIY-YIG domain that is generally found within a class of homing endonucleases. HIGLE directly interacts with the microprocessor components HYL1 and SE. Unlike the functions of other GIY-YIG endonucleases, the catalytic core of HIGLE has both DNase and RNase activities that sufficiently processes miRNA precursors into short fragments in vitro.

Original language	English
Pages (from-to)	1383-1393
Number of pages	11
Journal	FEBS Letters
Volume	591
Issue number	10
DOIs	https://doi.org/10.1002/1873-3468.12628
Publication status	Published - 2017 May 1

Bibliographical note

Funding Information:
This work was supported by grants from the National Center for Next-Generation BioGreen21 Program (PJ01194601), the National Research Foundation (NRF-2016R1C1B1013506 and NRF-2016K2A9A1A06922119), Yonsei University Research Fund (2016-12-0006), and ?Center of synthetic biology? funded by the UNIK Research Initiative of the Danish Ministry of Science, Technology and Innovation.

Publisher Copyright:
© 2017 Federation of European Biochemical Societies

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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Cho, Seok Keun ; Ryu, Moon Young ; Poulsen, Christian ; Kim, Jong Hum ; Oh, Tae Rin ; Choi, Suk Won ; Kim, Mijung ; Yang, Jun Yi ; Boo, Kyung Hwan ; Geshi, Naomi ; Kim, Woo Taek ; Yang, Seong Wook. / HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana. In: FEBS Letters. 2017 ; Vol. 591, No. 10. pp. 1383-1393.

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title = "HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana",

abstract = "A highly coordinated complex known as the microprocessor precisely processes primary transcripts of MIRNA genes into mature miRNAs. In plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE). To precisely modulate miRNA maturation, the microprocessor cooperates with at least 12 proteins in plants. In addition, we here show the involvement of a novel gene, HYL1-interacting GIY-YIG-like endonuclease (HIGLE). The encoded protein has a GIY-YIG domain that is generally found within a class of homing endonucleases. HIGLE directly interacts with the microprocessor components HYL1 and SE. Unlike the functions of other GIY-YIG endonucleases, the catalytic core of HIGLE has both DNase and RNase activities that sufficiently processes miRNA precursors into short fragments in vitro.",

author = "Cho, {Seok Keun} and Ryu, {Moon Young} and Christian Poulsen and Kim, {Jong Hum} and Oh, {Tae Rin} and Choi, {Suk Won} and Mijung Kim and Yang, {Jun Yi} and Boo, {Kyung Hwan} and Naomi Geshi and Kim, {Woo Taek} and Yang, {Seong Wook}",

note = "Funding Information: This work was supported by grants from the National Center for Next-Generation BioGreen21 Program (PJ01194601), the National Research Foundation (NRF-2016R1C1B1013506 and NRF-2016K2A9A1A06922119), Yonsei University Research Fund (2016-12-0006), and ?Center of synthetic biology? funded by the UNIK Research Initiative of the Danish Ministry of Science, Technology and Innovation. Publisher Copyright: {\textcopyright} 2017 Federation of European Biochemical Societies",

year = "2017",

month = may,

day = "1",

doi = "10.1002/1873-3468.12628",

language = "English",

volume = "591",

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HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana. / Cho, Seok Keun; Ryu, Moon Young; Poulsen, Christian; Kim, Jong Hum; Oh, Tae Rin; Choi, Suk Won; Kim, Mijung; Yang, Jun Yi; Boo, Kyung Hwan; Geshi, Naomi; Kim, Woo Taek ; Yang, Seong Wook.

In: FEBS Letters, Vol. 591, No. 10, 01.05.2017, p. 1383-1393.

Research output: Contribution to journal › Article › peer-review

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AU - Cho, Seok Keun

AU - Ryu, Moon Young

AU - Poulsen, Christian

AU - Kim, Jong Hum

AU - Oh, Tae Rin

AU - Choi, Suk Won

AU - Kim, Mijung

AU - Yang, Jun Yi

AU - Boo, Kyung Hwan

AU - Geshi, Naomi

AU - Kim, Woo Taek

AU - Yang, Seong Wook

N1 - Funding Information: This work was supported by grants from the National Center for Next-Generation BioGreen21 Program (PJ01194601), the National Research Foundation (NRF-2016R1C1B1013506 and NRF-2016K2A9A1A06922119), Yonsei University Research Fund (2016-12-0006), and ?Center of synthetic biology? funded by the UNIK Research Initiative of the Danish Ministry of Science, Technology and Innovation. Publisher Copyright: © 2017 Federation of European Biochemical Societies

PY - 2017/5/1

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N2 - A highly coordinated complex known as the microprocessor precisely processes primary transcripts of MIRNA genes into mature miRNAs. In plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE). To precisely modulate miRNA maturation, the microprocessor cooperates with at least 12 proteins in plants. In addition, we here show the involvement of a novel gene, HYL1-interacting GIY-YIG-like endonuclease (HIGLE). The encoded protein has a GIY-YIG domain that is generally found within a class of homing endonucleases. HIGLE directly interacts with the microprocessor components HYL1 and SE. Unlike the functions of other GIY-YIG endonucleases, the catalytic core of HIGLE has both DNase and RNase activities that sufficiently processes miRNA precursors into short fragments in vitro.

AB - A highly coordinated complex known as the microprocessor precisely processes primary transcripts of MIRNA genes into mature miRNAs. In plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE). To precisely modulate miRNA maturation, the microprocessor cooperates with at least 12 proteins in plants. In addition, we here show the involvement of a novel gene, HYL1-interacting GIY-YIG-like endonuclease (HIGLE). The encoded protein has a GIY-YIG domain that is generally found within a class of homing endonucleases. HIGLE directly interacts with the microprocessor components HYL1 and SE. Unlike the functions of other GIY-YIG endonucleases, the catalytic core of HIGLE has both DNase and RNase activities that sufficiently processes miRNA precursors into short fragments in vitro.

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HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana

Abstract

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