HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana

Seok Keun Cho, Moon Young Ryu, Christian Poulsen, Jong Hum Kim, Tae Rin Oh, Suk Won Choi, Mijung Kim, Jun Yi Yang, Kyung Hwan Boo, Naomi Geshi, Woo Taek Kim, Seong Wook Yang

Research output: Contribution to journalArticle

Abstract

A highly coordinated complex known as the microprocessor precisely processes primary transcripts of MIRNA genes into mature miRNAs. In plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE). To precisely modulate miRNA maturation, the microprocessor cooperates with at least 12 proteins in plants. In addition, we here show the involvement of a novel gene, HYL1-interacting GIY-YIG-like endonuclease (HIGLE). The encoded protein has a GIY-YIG domain that is generally found within a class of homing endonucleases. HIGLE directly interacts with the microprocessor components HYL1 and SE. Unlike the functions of other GIY-YIG endonucleases, the catalytic core of HIGLE has both DNase and RNase activities that sufficiently processes miRNA precursors into short fragments in vitro.

Original languageEnglish
Pages (from-to)1383-1393
Number of pages11
JournalFEBS Letters
Volume591
Issue number10
DOIs
Publication statusPublished - 2017 May 1

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Cho, S. K., Ryu, M. Y., Poulsen, C., Kim, J. H., Oh, T. R., Choi, S. W., Kim, M., Yang, J. Y., Boo, K. H., Geshi, N., Kim, W. T., & Yang, S. W. (2017). HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana. FEBS Letters, 591(10), 1383-1393. https://doi.org/10.1002/1873-3468.12628