HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana

Seok Keun Cho, Moon Young Ryu, Christian Poulsen, Jong Hum Kim, Tae Rin Oh, Suk Won Choi, Mijung Kim, Jun Yi Yang, Kyung Hwan Boo, Naomi Geshi, Woo Taek Kim, Seong Wook Yang

Research output: Contribution to journalLetter

Abstract

A highly coordinated complex known as the microprocessor precisely processes primary transcripts of MIRNA genes into mature miRNAs. In plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE). To precisely modulate miRNA maturation, the microprocessor cooperates with at least 12 proteins in plants. In addition, we here show the involvement of a novel gene, HYL1-interacting GIY-YIG-like endonuclease (HIGLE). The encoded protein has a GIY-YIG domain that is generally found within a class of homing endonucleases. HIGLE directly interacts with the microprocessor components HYL1 and SE. Unlike the functions of other GIY-YIG endonucleases, the catalytic core of HIGLE has both DNase and RNase activities that sufficiently processes miRNA precursors into short fragments in vitro.

Original languageEnglish
Pages (from-to)1383-1393
Number of pages11
JournalFEBS Letters
Volume591
Issue number10
DOIs
Publication statusPublished - 2017 May 1

Fingerprint

Endonucleases
Arabidopsis
Microcomputers
Microprocessor chips
MicroRNAs
Genes
Plant Proteins
Proteins
Deoxyribonucleases
Ribonucleases
Catalytic Domain

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cho, Seok Keun ; Ryu, Moon Young ; Poulsen, Christian ; Kim, Jong Hum ; Oh, Tae Rin ; Choi, Suk Won ; Kim, Mijung ; Yang, Jun Yi ; Boo, Kyung Hwan ; Geshi, Naomi ; Kim, Woo Taek ; Yang, Seong Wook. / HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana. In: FEBS Letters. 2017 ; Vol. 591, No. 10. pp. 1383-1393.
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abstract = "A highly coordinated complex known as the microprocessor precisely processes primary transcripts of MIRNA genes into mature miRNAs. In plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE). To precisely modulate miRNA maturation, the microprocessor cooperates with at least 12 proteins in plants. In addition, we here show the involvement of a novel gene, HYL1-interacting GIY-YIG-like endonuclease (HIGLE). The encoded protein has a GIY-YIG domain that is generally found within a class of homing endonucleases. HIGLE directly interacts with the microprocessor components HYL1 and SE. Unlike the functions of other GIY-YIG endonucleases, the catalytic core of HIGLE has both DNase and RNase activities that sufficiently processes miRNA precursors into short fragments in vitro.",
author = "Cho, {Seok Keun} and Ryu, {Moon Young} and Christian Poulsen and Kim, {Jong Hum} and Oh, {Tae Rin} and Choi, {Suk Won} and Mijung Kim and Yang, {Jun Yi} and Boo, {Kyung Hwan} and Naomi Geshi and Kim, {Woo Taek} and Yang, {Seong Wook}",
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Cho, SK, Ryu, MY, Poulsen, C, Kim, JH, Oh, TR, Choi, SW, Kim, M, Yang, JY, Boo, KH, Geshi, N, Kim, WT & Yang, SW 2017, 'HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana', FEBS Letters, vol. 591, no. 10, pp. 1383-1393. https://doi.org/10.1002/1873-3468.12628

HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana. / Cho, Seok Keun; Ryu, Moon Young; Poulsen, Christian; Kim, Jong Hum; Oh, Tae Rin; Choi, Suk Won; Kim, Mijung; Yang, Jun Yi; Boo, Kyung Hwan; Geshi, Naomi; Kim, Woo Taek; Yang, Seong Wook.

In: FEBS Letters, Vol. 591, No. 10, 01.05.2017, p. 1383-1393.

Research output: Contribution to journalLetter

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AU - Cho, Seok Keun

AU - Ryu, Moon Young

AU - Poulsen, Christian

AU - Kim, Jong Hum

AU - Oh, Tae Rin

AU - Choi, Suk Won

AU - Kim, Mijung

AU - Yang, Jun Yi

AU - Boo, Kyung Hwan

AU - Geshi, Naomi

AU - Kim, Woo Taek

AU - Yang, Seong Wook

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