Histamine-induced Ca2+ influx via the PLA2/ lipoxygenase/TRPV1 pathway in rat sensory neurons

Byungmoon Kim, Sang Hee Lee, Won Sik Shim, Uhtaek Oh

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Histamine is known to excite a subset of C-fibers and cause itch sensation. Despite its well-defined excitatory action on sensory neurons, intracellular signaling mechanisms are not understood. Previously, we demonstrated that bradykinin excited sensory neurons by activating TRPV1 via the phospholipase A2 (PLA2) and lipoxygenase (LO) pathway. We, thus, hypothesized that histamine excited sensory neurons via the PLA 2/LO/TRPV1 pathway. Application of histamine elicited a rapid increase in intracellular Ca2+ ([Ca2+]i) that desensitized slowly in cultured dorsal root ganglion neurons. Histamine-induced [Ca2+]i was dependent on extracellular Ca2+ and inhibited by capsazepine and by SC0030, competitive antagonists of TRPV1. Quinacrine and nordihydroguaiaretic acid, a PLA2 and an LO inhibitor, respectively, blocked the histamine-induced Ca2+ influx in sensory neurons, while indomethacin (a cyclooxygenase inhibitor) did not. We thus conclude that histamine activates TRPV1 after stimulating the PLA2/LO pathway, leading to the excitation of sensory neurons. These results further provide an idea for potential use of TRPV1 antagonists as anti-itch drugs.

Original languageEnglish
Pages (from-to)159-162
Number of pages4
JournalNeuroscience Letters
Volume361
Issue number1-3
DOIs
Publication statusPublished - 2004 May 6

Fingerprint

Lipoxygenase
Phospholipases A2
Sensory Receptor Cells
Histamine
Masoprocol
Quinacrine
Unmyelinated Nerve Fibers
Cyclooxygenase Inhibitors
Spinal Ganglia
Bradykinin
Indomethacin
Neurons
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Kim, Byungmoon ; Lee, Sang Hee ; Shim, Won Sik ; Oh, Uhtaek. / Histamine-induced Ca2+ influx via the PLA2/ lipoxygenase/TRPV1 pathway in rat sensory neurons. In: Neuroscience Letters. 2004 ; Vol. 361, No. 1-3. pp. 159-162.
@article{9bae5369bc2448c8bfb1477951a60f85,
title = "Histamine-induced Ca2+ influx via the PLA2/ lipoxygenase/TRPV1 pathway in rat sensory neurons",
abstract = "Histamine is known to excite a subset of C-fibers and cause itch sensation. Despite its well-defined excitatory action on sensory neurons, intracellular signaling mechanisms are not understood. Previously, we demonstrated that bradykinin excited sensory neurons by activating TRPV1 via the phospholipase A2 (PLA2) and lipoxygenase (LO) pathway. We, thus, hypothesized that histamine excited sensory neurons via the PLA 2/LO/TRPV1 pathway. Application of histamine elicited a rapid increase in intracellular Ca2+ ([Ca2+]i) that desensitized slowly in cultured dorsal root ganglion neurons. Histamine-induced [Ca2+]i was dependent on extracellular Ca2+ and inhibited by capsazepine and by SC0030, competitive antagonists of TRPV1. Quinacrine and nordihydroguaiaretic acid, a PLA2 and an LO inhibitor, respectively, blocked the histamine-induced Ca2+ influx in sensory neurons, while indomethacin (a cyclooxygenase inhibitor) did not. We thus conclude that histamine activates TRPV1 after stimulating the PLA2/LO pathway, leading to the excitation of sensory neurons. These results further provide an idea for potential use of TRPV1 antagonists as anti-itch drugs.",
author = "Byungmoon Kim and Lee, {Sang Hee} and Shim, {Won Sik} and Uhtaek Oh",
year = "2004",
month = "5",
day = "6",
doi = "10.1016/j.neulet.2004.01.019",
language = "English",
volume = "361",
pages = "159--162",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1-3",

}

Histamine-induced Ca2+ influx via the PLA2/ lipoxygenase/TRPV1 pathway in rat sensory neurons. / Kim, Byungmoon; Lee, Sang Hee; Shim, Won Sik; Oh, Uhtaek.

In: Neuroscience Letters, Vol. 361, No. 1-3, 06.05.2004, p. 159-162.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Histamine-induced Ca2+ influx via the PLA2/ lipoxygenase/TRPV1 pathway in rat sensory neurons

AU - Kim, Byungmoon

AU - Lee, Sang Hee

AU - Shim, Won Sik

AU - Oh, Uhtaek

PY - 2004/5/6

Y1 - 2004/5/6

N2 - Histamine is known to excite a subset of C-fibers and cause itch sensation. Despite its well-defined excitatory action on sensory neurons, intracellular signaling mechanisms are not understood. Previously, we demonstrated that bradykinin excited sensory neurons by activating TRPV1 via the phospholipase A2 (PLA2) and lipoxygenase (LO) pathway. We, thus, hypothesized that histamine excited sensory neurons via the PLA 2/LO/TRPV1 pathway. Application of histamine elicited a rapid increase in intracellular Ca2+ ([Ca2+]i) that desensitized slowly in cultured dorsal root ganglion neurons. Histamine-induced [Ca2+]i was dependent on extracellular Ca2+ and inhibited by capsazepine and by SC0030, competitive antagonists of TRPV1. Quinacrine and nordihydroguaiaretic acid, a PLA2 and an LO inhibitor, respectively, blocked the histamine-induced Ca2+ influx in sensory neurons, while indomethacin (a cyclooxygenase inhibitor) did not. We thus conclude that histamine activates TRPV1 after stimulating the PLA2/LO pathway, leading to the excitation of sensory neurons. These results further provide an idea for potential use of TRPV1 antagonists as anti-itch drugs.

AB - Histamine is known to excite a subset of C-fibers and cause itch sensation. Despite its well-defined excitatory action on sensory neurons, intracellular signaling mechanisms are not understood. Previously, we demonstrated that bradykinin excited sensory neurons by activating TRPV1 via the phospholipase A2 (PLA2) and lipoxygenase (LO) pathway. We, thus, hypothesized that histamine excited sensory neurons via the PLA 2/LO/TRPV1 pathway. Application of histamine elicited a rapid increase in intracellular Ca2+ ([Ca2+]i) that desensitized slowly in cultured dorsal root ganglion neurons. Histamine-induced [Ca2+]i was dependent on extracellular Ca2+ and inhibited by capsazepine and by SC0030, competitive antagonists of TRPV1. Quinacrine and nordihydroguaiaretic acid, a PLA2 and an LO inhibitor, respectively, blocked the histamine-induced Ca2+ influx in sensory neurons, while indomethacin (a cyclooxygenase inhibitor) did not. We thus conclude that histamine activates TRPV1 after stimulating the PLA2/LO pathway, leading to the excitation of sensory neurons. These results further provide an idea for potential use of TRPV1 antagonists as anti-itch drugs.

UR - http://www.scopus.com/inward/record.url?scp=2342466078&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2342466078&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2004.01.019

DO - 10.1016/j.neulet.2004.01.019

M3 - Article

VL - 361

SP - 159

EP - 162

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1-3

ER -