Histologic features of acute thrombi retrieved from stroke patients during mechanical reperfusion therapy

Seong Hwan Ahn, Ran Hong, In Sung Choo, Jihoe Heo, Hyo Suk Nam, Hyun Goo Kang, Hoo Won Kim, Jin Ho Kim

Research output: Contribution to journalArticle

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Abstract

Background: The histologic features of thrombus may differ according to the stroke subtypes. However, in acute reperfusion therapy, fibrin-specific thrombolytics are used based on the assumption that all thrombi are alike. Aims: The histologic characteristics of thrombi were compared between patients with different stroke etiologies. Methods: Between April 2010 and March 2012, we analyzed thrombi retrieved from acute stroke patients during mechanical thrombectomy. All thrombi were analyzed using component-specific stains such as Martius scarlet blue for fibrins and immunostaining with CD42b antibody for platelets. The stroke subtypes were determined based on the Trial of ORG 10172 in Acute Stroke Treatment classification. Results: Among 36 patients, 22 were diagnosed with cardioembolism, 8 with atherothrombosis, and 6 with undetermined etiology. In arteriogenic thrombi, red blood cells were most abundant (56.9 ± 12.2%), and the platelets covered the fibrin layers or were localized at the edge or periphery of the thrombus. In cardiogenic thrombi, fibrin was most abundant (39.5 ± 13.5%), and platelets were clustered within the rich fibrin. Red blood cells proportion was greater in arteriogenic thrombi than in cardiogenic thrombi (p < 0.001), whereas fibrin proportion was greater in cardiogenic thrombi than in arteriogenic thrombi (p = 0.003). Of six patients with undetermined etiology, the thrombi in five showed histologic features and composition similar to that of cardiogenic thrombi. Conclusions: Acute thrombi showed different histologic features according to the stroke etiology. The distribution of platelets and proportion of red blood cells and fibrin were major distinguishing factors between stroke subtypes.

Original languageEnglish
Pages (from-to)1036-1044
Number of pages9
JournalInternational Journal of Stroke
Volume11
Issue number9
DOIs
Publication statusPublished - 2016 Dec 1

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Reperfusion
Thrombosis
Stroke
Fibrin
Therapeutics
Blood Platelets
Erythrocytes
Thrombectomy
Coloring Agents

All Science Journal Classification (ASJC) codes

  • Neurology

Cite this

Ahn, Seong Hwan ; Hong, Ran ; Choo, In Sung ; Heo, Jihoe ; Nam, Hyo Suk ; Kang, Hyun Goo ; Kim, Hoo Won ; Kim, Jin Ho. / Histologic features of acute thrombi retrieved from stroke patients during mechanical reperfusion therapy. In: International Journal of Stroke. 2016 ; Vol. 11, No. 9. pp. 1036-1044.
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abstract = "Background: The histologic features of thrombus may differ according to the stroke subtypes. However, in acute reperfusion therapy, fibrin-specific thrombolytics are used based on the assumption that all thrombi are alike. Aims: The histologic characteristics of thrombi were compared between patients with different stroke etiologies. Methods: Between April 2010 and March 2012, we analyzed thrombi retrieved from acute stroke patients during mechanical thrombectomy. All thrombi were analyzed using component-specific stains such as Martius scarlet blue for fibrins and immunostaining with CD42b antibody for platelets. The stroke subtypes were determined based on the Trial of ORG 10172 in Acute Stroke Treatment classification. Results: Among 36 patients, 22 were diagnosed with cardioembolism, 8 with atherothrombosis, and 6 with undetermined etiology. In arteriogenic thrombi, red blood cells were most abundant (56.9 ± 12.2{\%}), and the platelets covered the fibrin layers or were localized at the edge or periphery of the thrombus. In cardiogenic thrombi, fibrin was most abundant (39.5 ± 13.5{\%}), and platelets were clustered within the rich fibrin. Red blood cells proportion was greater in arteriogenic thrombi than in cardiogenic thrombi (p < 0.001), whereas fibrin proportion was greater in cardiogenic thrombi than in arteriogenic thrombi (p = 0.003). Of six patients with undetermined etiology, the thrombi in five showed histologic features and composition similar to that of cardiogenic thrombi. Conclusions: Acute thrombi showed different histologic features according to the stroke etiology. The distribution of platelets and proportion of red blood cells and fibrin were major distinguishing factors between stroke subtypes.",
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Histologic features of acute thrombi retrieved from stroke patients during mechanical reperfusion therapy. / Ahn, Seong Hwan; Hong, Ran; Choo, In Sung; Heo, Jihoe; Nam, Hyo Suk; Kang, Hyun Goo; Kim, Hoo Won; Kim, Jin Ho.

In: International Journal of Stroke, Vol. 11, No. 9, 01.12.2016, p. 1036-1044.

Research output: Contribution to journalArticle

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AU - Ahn, Seong Hwan

AU - Hong, Ran

AU - Choo, In Sung

AU - Heo, Jihoe

AU - Nam, Hyo Suk

AU - Kang, Hyun Goo

AU - Kim, Hoo Won

AU - Kim, Jin Ho

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Y1 - 2016/12/1

N2 - Background: The histologic features of thrombus may differ according to the stroke subtypes. However, in acute reperfusion therapy, fibrin-specific thrombolytics are used based on the assumption that all thrombi are alike. Aims: The histologic characteristics of thrombi were compared between patients with different stroke etiologies. Methods: Between April 2010 and March 2012, we analyzed thrombi retrieved from acute stroke patients during mechanical thrombectomy. All thrombi were analyzed using component-specific stains such as Martius scarlet blue for fibrins and immunostaining with CD42b antibody for platelets. The stroke subtypes were determined based on the Trial of ORG 10172 in Acute Stroke Treatment classification. Results: Among 36 patients, 22 were diagnosed with cardioembolism, 8 with atherothrombosis, and 6 with undetermined etiology. In arteriogenic thrombi, red blood cells were most abundant (56.9 ± 12.2%), and the platelets covered the fibrin layers or were localized at the edge or periphery of the thrombus. In cardiogenic thrombi, fibrin was most abundant (39.5 ± 13.5%), and platelets were clustered within the rich fibrin. Red blood cells proportion was greater in arteriogenic thrombi than in cardiogenic thrombi (p < 0.001), whereas fibrin proportion was greater in cardiogenic thrombi than in arteriogenic thrombi (p = 0.003). Of six patients with undetermined etiology, the thrombi in five showed histologic features and composition similar to that of cardiogenic thrombi. Conclusions: Acute thrombi showed different histologic features according to the stroke etiology. The distribution of platelets and proportion of red blood cells and fibrin were major distinguishing factors between stroke subtypes.

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