Histone H3K27 Demethylase JMJD3 in Cooperation with NF-κB Regulates Keratinocyte Wound Healing

Jungtae Na, Kwanghyun Lee, Wonho Na, Jee Yoon Shin, Min Jung Lee, Tae Young Yune, Hae Kwang Lee, Hansung Jung, Won Sun Kim, Bong Gun Ju

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Histone H3K27me3 demethylase JMJD3 has been shown to be involved in keratinocyte differentiation and wound healing. However, the exact molecular mechanism underlying JMJD3-mediated keratinocyte wound healing has not been fully elucidated. In this study, we report on the biological function of JMJD3 in keratinocyte wound healing using in vitro cell and in vivo animal models. Our results indicate that JMJD3 up-regulation and NF-κB activation occur in the region of the wound edge during keratinocyte wound healing. We also found that JMJD3 interacts with NF-κB, resulting in increased expression of the inflammatory, matrix metalloproteinase, and growth factor genes via demethylation of H3K27me3 at the gene promoters. Consistently, inactivation of JMJD3 or NF-κB resulted in aberrant keratinocyte wound healing. Our study suggests that regulation of JMJD3 may provide a new therapeutic intervention for treating the chronic skin wound.

Original languageEnglish
Pages (from-to)847-858
Number of pages12
JournalJournal of Investigative Dermatology
Volume136
Issue number4
DOIs
Publication statusPublished - 2016 Jan 1

Fingerprint

Histone Demethylases
Keratinocytes
Wound Healing
Genes
Wounds and Injuries
Matrix Metalloproteinases
Intercellular Signaling Peptides and Proteins
Skin
Animals
Up-Regulation
Animal Models
Chemical activation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Na, Jungtae ; Lee, Kwanghyun ; Na, Wonho ; Shin, Jee Yoon ; Lee, Min Jung ; Yune, Tae Young ; Lee, Hae Kwang ; Jung, Hansung ; Kim, Won Sun ; Ju, Bong Gun. / Histone H3K27 Demethylase JMJD3 in Cooperation with NF-κB Regulates Keratinocyte Wound Healing. In: Journal of Investigative Dermatology. 2016 ; Vol. 136, No. 4. pp. 847-858.
@article{bcedad2127714ee8bb6e3ab6cc5af32c,
title = "Histone H3K27 Demethylase JMJD3 in Cooperation with NF-κB Regulates Keratinocyte Wound Healing",
abstract = "Histone H3K27me3 demethylase JMJD3 has been shown to be involved in keratinocyte differentiation and wound healing. However, the exact molecular mechanism underlying JMJD3-mediated keratinocyte wound healing has not been fully elucidated. In this study, we report on the biological function of JMJD3 in keratinocyte wound healing using in vitro cell and in vivo animal models. Our results indicate that JMJD3 up-regulation and NF-κB activation occur in the region of the wound edge during keratinocyte wound healing. We also found that JMJD3 interacts with NF-κB, resulting in increased expression of the inflammatory, matrix metalloproteinase, and growth factor genes via demethylation of H3K27me3 at the gene promoters. Consistently, inactivation of JMJD3 or NF-κB resulted in aberrant keratinocyte wound healing. Our study suggests that regulation of JMJD3 may provide a new therapeutic intervention for treating the chronic skin wound.",
author = "Jungtae Na and Kwanghyun Lee and Wonho Na and Shin, {Jee Yoon} and Lee, {Min Jung} and Yune, {Tae Young} and Lee, {Hae Kwang} and Hansung Jung and Kim, {Won Sun} and Ju, {Bong Gun}",
year = "2016",
month = "1",
day = "1",
doi = "10.1016/j.jid.2015.11.029",
language = "English",
volume = "136",
pages = "847--858",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "4",

}

Histone H3K27 Demethylase JMJD3 in Cooperation with NF-κB Regulates Keratinocyte Wound Healing. / Na, Jungtae; Lee, Kwanghyun; Na, Wonho; Shin, Jee Yoon; Lee, Min Jung; Yune, Tae Young; Lee, Hae Kwang; Jung, Hansung; Kim, Won Sun; Ju, Bong Gun.

In: Journal of Investigative Dermatology, Vol. 136, No. 4, 01.01.2016, p. 847-858.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Histone H3K27 Demethylase JMJD3 in Cooperation with NF-κB Regulates Keratinocyte Wound Healing

AU - Na, Jungtae

AU - Lee, Kwanghyun

AU - Na, Wonho

AU - Shin, Jee Yoon

AU - Lee, Min Jung

AU - Yune, Tae Young

AU - Lee, Hae Kwang

AU - Jung, Hansung

AU - Kim, Won Sun

AU - Ju, Bong Gun

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Histone H3K27me3 demethylase JMJD3 has been shown to be involved in keratinocyte differentiation and wound healing. However, the exact molecular mechanism underlying JMJD3-mediated keratinocyte wound healing has not been fully elucidated. In this study, we report on the biological function of JMJD3 in keratinocyte wound healing using in vitro cell and in vivo animal models. Our results indicate that JMJD3 up-regulation and NF-κB activation occur in the region of the wound edge during keratinocyte wound healing. We also found that JMJD3 interacts with NF-κB, resulting in increased expression of the inflammatory, matrix metalloproteinase, and growth factor genes via demethylation of H3K27me3 at the gene promoters. Consistently, inactivation of JMJD3 or NF-κB resulted in aberrant keratinocyte wound healing. Our study suggests that regulation of JMJD3 may provide a new therapeutic intervention for treating the chronic skin wound.

AB - Histone H3K27me3 demethylase JMJD3 has been shown to be involved in keratinocyte differentiation and wound healing. However, the exact molecular mechanism underlying JMJD3-mediated keratinocyte wound healing has not been fully elucidated. In this study, we report on the biological function of JMJD3 in keratinocyte wound healing using in vitro cell and in vivo animal models. Our results indicate that JMJD3 up-regulation and NF-κB activation occur in the region of the wound edge during keratinocyte wound healing. We also found that JMJD3 interacts with NF-κB, resulting in increased expression of the inflammatory, matrix metalloproteinase, and growth factor genes via demethylation of H3K27me3 at the gene promoters. Consistently, inactivation of JMJD3 or NF-κB resulted in aberrant keratinocyte wound healing. Our study suggests that regulation of JMJD3 may provide a new therapeutic intervention for treating the chronic skin wound.

UR - http://www.scopus.com/inward/record.url?scp=84960843160&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84960843160&partnerID=8YFLogxK

U2 - 10.1016/j.jid.2015.11.029

DO - 10.1016/j.jid.2015.11.029

M3 - Article

VL - 136

SP - 847

EP - 858

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 4

ER -