HMGB1 in the pathogenesis of ultraviolet-induced ocular surface inflammation

S. J. Han, H. J. Min, S. C. Yoon, E. A. Ko, S. J. Park, J. H. Yoon, J. S. Shin, KyoungYul Seo

Research output: Contribution to journalArticle

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Abstract

High-mobility group box 1 (HMGB1) functions as a transcription-enhancing nuclear protein as well as a crucial cytokine that regulates inflammation. This study demonstrated that secretion of HMGB1 due to ultraviolet (UV) radiation inducing ocular surface inflammation-mediated reactive oxygen species (ROS) production. After treating conjunctival epithelial cells with UV radiation, HMGB1 was translocated from the nucleus to the cytoplasm and then eventually to the extracellular space. HMGB1 played a crucial role in UV-induced conjunctival neutrophil infiltration, which subsided when mice were pretreated with the HMGB1 inhibitors soluble receptor for advanced glycation endproducts (sRAGEs) and HMGB1 A box protein. In case of using ROS quencher, there was decrease in UV-induced HMGB1 secretion in conjunctival epithelial cells and mice. Considering that UV-induced chronic inflammation causes ocular surface change as pterygium, we have confirmed high HMGB1 translocation and ROS expression in human pterygium. Our findings therefore revealed a previously unknown mechanism of UV-induced ocular inflammation related to ROS and HMGB1 suggesting a new medical therapeutic target.

Original languageEnglish
Article numbere1863
JournalCell Death and Disease
Volume6
Issue number8
DOIs
Publication statusPublished - 2015 Aug 27

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Reactive Oxygen Species
Inflammation
Pterygium
Epithelial Cells
Radiation
Neutrophil Infiltration
Extracellular Space
Nuclear Proteins
Cytoplasm
Cytokines
Proteins
Therapeutics

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Cancer Research

Cite this

Han, S. J., Min, H. J., Yoon, S. C., Ko, E. A., Park, S. J., Yoon, J. H., ... Seo, K. (2015). HMGB1 in the pathogenesis of ultraviolet-induced ocular surface inflammation. Cell Death and Disease, 6(8), [e1863]. https://doi.org/10.1038/cddis.2015.199
Han, S. J. ; Min, H. J. ; Yoon, S. C. ; Ko, E. A. ; Park, S. J. ; Yoon, J. H. ; Shin, J. S. ; Seo, KyoungYul. / HMGB1 in the pathogenesis of ultraviolet-induced ocular surface inflammation. In: Cell Death and Disease. 2015 ; Vol. 6, No. 8.
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Han, SJ, Min, HJ, Yoon, SC, Ko, EA, Park, SJ, Yoon, JH, Shin, JS & Seo, K 2015, 'HMGB1 in the pathogenesis of ultraviolet-induced ocular surface inflammation', Cell Death and Disease, vol. 6, no. 8, e1863. https://doi.org/10.1038/cddis.2015.199

HMGB1 in the pathogenesis of ultraviolet-induced ocular surface inflammation. / Han, S. J.; Min, H. J.; Yoon, S. C.; Ko, E. A.; Park, S. J.; Yoon, J. H.; Shin, J. S.; Seo, KyoungYul.

In: Cell Death and Disease, Vol. 6, No. 8, e1863, 27.08.2015.

Research output: Contribution to journalArticle

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