Hollow-fiber flow field-flow fractionation: A pipeline to scale down separation and enhance detection of proteins and cells

Pierluigi Reschiglian, Andrea Zattoni, Barbara Roda, Diana C. Rambaldi, Myeong Hee Moon

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Commercial flow field-flow fractionation (FlFFF) employs macro-scale, flat-type channels. The idea of hollow-fiber (HF) membranes as tubular, micro-column channels for FlFFF (HF FlFFF or, more shortly, HF5) dates back to 1974, with fundamentals on HF5 given in the late 1980s, and outstanding applications reported only over the last 15 years.Compared to flat-channel FlFFF, the key aspect of HF5 lies in the downscaling of the fractionation channel. This implies low-cost, possible disposable usage, and low volume of the channel that allows on-line coupling with highly sensitive detection and characterization techniques. The use of coupled techniques enhances the analysis of macromolecules and micron-sized particles such as intact proteins and whole cells. In this chapter we first report a few basics on HF5 theory and instrumentation. We then focus on technical and methodological developments that have made HF5 reach a performance normally achieved by flat-channel FlFFF. We finally focus on the enhancements obtained by coupling HF5 with powerful methods for detection and characterization of intact proteins and whole cells such as multi-angle light scattering (MALS), time-of-flight (TOF) mass spectrometry (MS), chemiluminescence (CL), and UV/Vis turbidity diode-array detection (DAD).

Original languageEnglish
Title of host publicationField-Flow Fractionation in Biopolymer Analysis
PublisherSpringer-Verlag Wien
Pages37-56
Number of pages20
ISBN (Electronic)9783709101544
ISBN (Print)9783709101537
DOIs
Publication statusPublished - 2012 Jan 1

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All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Reschiglian, P., Zattoni, A., Roda, B., Rambaldi, D. C., & Moon, M. H. (2012). Hollow-fiber flow field-flow fractionation: A pipeline to scale down separation and enhance detection of proteins and cells. In Field-Flow Fractionation in Biopolymer Analysis (pp. 37-56). Springer-Verlag Wien. https://doi.org/10.1007/978-3-7091-0154-4_3