TY - GEN
T1 - Hollow-fiber flow field-flow fractionation for mass spectrometry
T2 - From proteins to whole bacteria
AU - Reschiglian, Pierluigi
AU - Zattoni, Andrea
AU - Rambaldi, Diana Cristina
AU - Roda, Aldo
AU - Hee Moon, Myeong
PY - 2011
Y1 - 2011
N2 - Mass spectrometry (MS) provides analyte identification over a wide molar-mass range. However, particularly in the case of complex matrices, this ability is often enhanced by the use of pre-MS separation steps. A separation, prototype technique for the "gentle" fractionation of large/ultralarge analytes, from proteins to whole cells, is here described to reduce complexity and maintain native characteristics of the sample before MS analysis. It is based on flow field-flow fractionation, and it employs a micro-volume fractionation channel made of a ca. 20 cm hollow-fiber membrane of sub-millimeter section. The key advantages of this technique lie in the low volume and low-cost of the channel, which makes it suitable to a disposable usage. Fractionation performance and instrumental simplicity make it an interesting methodology for in-batch or on-line pre-MS treatment of such samples.
AB - Mass spectrometry (MS) provides analyte identification over a wide molar-mass range. However, particularly in the case of complex matrices, this ability is often enhanced by the use of pre-MS separation steps. A separation, prototype technique for the "gentle" fractionation of large/ultralarge analytes, from proteins to whole cells, is here described to reduce complexity and maintain native characteristics of the sample before MS analysis. It is based on flow field-flow fractionation, and it employs a micro-volume fractionation channel made of a ca. 20 cm hollow-fiber membrane of sub-millimeter section. The key advantages of this technique lie in the low volume and low-cost of the channel, which makes it suitable to a disposable usage. Fractionation performance and instrumental simplicity make it an interesting methodology for in-batch or on-line pre-MS treatment of such samples.
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U2 - 10.1007/978-90-481-9815-3_2
DO - 10.1007/978-90-481-9815-3_2
M3 - Conference contribution
AN - SCOPUS:78650505154
SN - 9789048198146
T3 - NATO Science for Peace and Security Series A: Chemistry and Biology
SP - 13
EP - 36
BT - Detection of Biological Agents for the Prevention of Bioterrorism
PB - Springer Verlag
ER -