Hollow-fiber flow field-flow fractionation for mass spectrometry: From proteins to whole bacteria

Pierluigi Reschiglian, Andrea Zattoni, Diana Cristina Rambaldi, Aldo Roda, Myeong Hee Moon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)


Mass spectrometry (MS) provides analyte identification over a wide molar-mass range. However, particularly in the case of complex matrices, this ability is often enhanced by the use of pre-MS separation steps. A separation, prototype technique for the "gentle" fractionation of large/ultralarge analytes, from proteins to whole cells, is here described to reduce complexity and maintain native characteristics of the sample before MS analysis. It is based on flow field-flow fractionation, and it employs a micro-volume fractionation channel made of a ca. 20 cm hollow-fiber membrane of sub-millimeter section. The key advantages of this technique lie in the low volume and low-cost of the channel, which makes it suitable to a disposable usage. Fractionation performance and instrumental simplicity make it an interesting methodology for in-batch or on-line pre-MS treatment of such samples.

Original languageEnglish
Title of host publicationDetection of Biological Agents for the Prevention of Bioterrorism
PublisherSpringer Verlag
Number of pages24
ISBN (Print)9789048198146
Publication statusPublished - 2011

Publication series

NameNATO Science for Peace and Security Series A: Chemistry and Biology
ISSN (Print)1874-6489

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Safety, Risk, Reliability and Quality

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