Hollow-fiber flow field-flow fractionation for mass spectrometry: From proteins to whole bacteria

Pierluigi Reschiglian; Andrea Zattoni; Diana Cristina Rambaldi; Aldo Roda; Myeong Hee Moon

doi:10.1007/978-90-481-9815-3_2

Hollow-fiber flow field-flow fractionation for mass spectrometry: From proteins to whole bacteria

Pierluigi Reschiglian, Andrea Zattoni, Diana Cristina Rambaldi, Aldo Roda, Myeong Hee Moon

Department of Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

Mass spectrometry (MS) provides analyte identification over a wide molar-mass range. However, particularly in the case of complex matrices, this ability is often enhanced by the use of pre-MS separation steps. A separation, prototype technique for the "gentle" fractionation of large/ultralarge analytes, from proteins to whole cells, is here described to reduce complexity and maintain native characteristics of the sample before MS analysis. It is based on flow field-flow fractionation, and it employs a micro-volume fractionation channel made of a ca. 20 cm hollow-fiber membrane of sub-millimeter section. The key advantages of this technique lie in the low volume and low-cost of the channel, which makes it suitable to a disposable usage. Fractionation performance and instrumental simplicity make it an interesting methodology for in-batch or on-line pre-MS treatment of such samples.

Original language	English
Title of host publication	Detection of Biological Agents for the Prevention of Bioterrorism
Publisher	Springer Verlag
Pages	13-36
Number of pages	24
ISBN (Print)	9789048198146
DOIs	https://doi.org/10.1007/978-90-481-9815-3_2
Publication status	Published - 2011

Publication series

Name	NATO Science for Peace and Security Series A: Chemistry and Biology
ISSN (Print)	1874-6489

Bibliographical note

Funding Information:
Work supported by the University of Bologna, the Italian Ministry of Education, University and Research, the Italian Ministry of Foreign Affair, and by the Korea Foundation for the International Cooperation of Science & Technology (KICOS). MHM thanks for the grant support from Korea Research Foundation (KRF-2008-313-C00567).

All Science Journal Classification (ASJC) codes

Chemistry(all)
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Safety, Risk, Reliability and Quality

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10.1007/978-90-481-9815-3_2

Cite this

Reschiglian, P., Zattoni, A., Rambaldi, D. C., Roda, A., & Hee Moon, M. (2011). Hollow-fiber flow field-flow fractionation for mass spectrometry: From proteins to whole bacteria. In Detection of Biological Agents for the Prevention of Bioterrorism (pp. 13-36). (NATO Science for Peace and Security Series A: Chemistry and Biology). Springer Verlag. https://doi.org/10.1007/978-90-481-9815-3_2

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title = "Hollow-fiber flow field-flow fractionation for mass spectrometry: From proteins to whole bacteria",

abstract = "Mass spectrometry (MS) provides analyte identification over a wide molar-mass range. However, particularly in the case of complex matrices, this ability is often enhanced by the use of pre-MS separation steps. A separation, prototype technique for the {"}gentle{"} fractionation of large/ultralarge analytes, from proteins to whole cells, is here described to reduce complexity and maintain native characteristics of the sample before MS analysis. It is based on flow field-flow fractionation, and it employs a micro-volume fractionation channel made of a ca. 20 cm hollow-fiber membrane of sub-millimeter section. The key advantages of this technique lie in the low volume and low-cost of the channel, which makes it suitable to a disposable usage. Fractionation performance and instrumental simplicity make it an interesting methodology for in-batch or on-line pre-MS treatment of such samples.",

author = "Pierluigi Reschiglian and Andrea Zattoni and Rambaldi, {Diana Cristina} and Aldo Roda and {Hee Moon}, Myeong",

note = "Funding Information: Work supported by the University of Bologna, the Italian Ministry of Education, University and Research, the Italian Ministry of Foreign Affair, and by the Korea Foundation for the International Cooperation of Science & Technology (KICOS). MHM thanks for the grant support from Korea Research Foundation (KRF-2008-313-C00567).",

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Reschiglian, P, Zattoni, A, Rambaldi, DC, Roda, A & Hee Moon, M 2011, Hollow-fiber flow field-flow fractionation for mass spectrometry: From proteins to whole bacteria. in Detection of Biological Agents for the Prevention of Bioterrorism. NATO Science for Peace and Security Series A: Chemistry and Biology, Springer Verlag, pp. 13-36. https://doi.org/10.1007/978-90-481-9815-3_2

Hollow-fiber flow field-flow fractionation for mass spectrometry : From proteins to whole bacteria. / Reschiglian, Pierluigi; Zattoni, Andrea; Rambaldi, Diana Cristina et al.

Detection of Biological Agents for the Prevention of Bioterrorism. Springer Verlag, 2011. p. 13-36 (NATO Science for Peace and Security Series A: Chemistry and Biology).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Rambaldi, Diana Cristina

AU - Roda, Aldo

AU - Hee Moon, Myeong

N1 - Funding Information: Work supported by the University of Bologna, the Italian Ministry of Education, University and Research, the Italian Ministry of Foreign Affair, and by the Korea Foundation for the International Cooperation of Science & Technology (KICOS). MHM thanks for the grant support from Korea Research Foundation (KRF-2008-313-C00567).

PY - 2011

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N2 - Mass spectrometry (MS) provides analyte identification over a wide molar-mass range. However, particularly in the case of complex matrices, this ability is often enhanced by the use of pre-MS separation steps. A separation, prototype technique for the "gentle" fractionation of large/ultralarge analytes, from proteins to whole cells, is here described to reduce complexity and maintain native characteristics of the sample before MS analysis. It is based on flow field-flow fractionation, and it employs a micro-volume fractionation channel made of a ca. 20 cm hollow-fiber membrane of sub-millimeter section. The key advantages of this technique lie in the low volume and low-cost of the channel, which makes it suitable to a disposable usage. Fractionation performance and instrumental simplicity make it an interesting methodology for in-batch or on-line pre-MS treatment of such samples.

AB - Mass spectrometry (MS) provides analyte identification over a wide molar-mass range. However, particularly in the case of complex matrices, this ability is often enhanced by the use of pre-MS separation steps. A separation, prototype technique for the "gentle" fractionation of large/ultralarge analytes, from proteins to whole cells, is here described to reduce complexity and maintain native characteristics of the sample before MS analysis. It is based on flow field-flow fractionation, and it employs a micro-volume fractionation channel made of a ca. 20 cm hollow-fiber membrane of sub-millimeter section. The key advantages of this technique lie in the low volume and low-cost of the channel, which makes it suitable to a disposable usage. Fractionation performance and instrumental simplicity make it an interesting methodology for in-batch or on-line pre-MS treatment of such samples.

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Reschiglian P, Zattoni A, Rambaldi DC, Roda A, Hee Moon M. Hollow-fiber flow field-flow fractionation for mass spectrometry: From proteins to whole bacteria. In Detection of Biological Agents for the Prevention of Bioterrorism. Springer Verlag. 2011. p. 13-36. (NATO Science for Peace and Security Series A: Chemistry and Biology). https://doi.org/10.1007/978-90-481-9815-3_2

Hollow-fiber flow field-flow fractionation for mass spectrometry: From proteins to whole bacteria

Abstract

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