Hollow-fiber flow field-flow fractionation for mass spectrometry: From proteins to whole bacteria

Pierluigi Reschiglian, Andrea Zattoni, Diana Cristina Rambaldi, Aldo Roda, Myeong Hee Moon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Mass spectrometry (MS) provides analyte identification over a wide molar-mass range. However, particularly in the case of complex matrices, this ability is often enhanced by the use of pre-MS separation steps. A separation, prototype technique for the "gentle" fractionation of large/ultralarge analytes, from proteins to whole cells, is here described to reduce complexity and maintain native characteristics of the sample before MS analysis. It is based on flow field-flow fractionation, and it employs a micro-volume fractionation channel made of a ca. 20 cm hollow-fiber membrane of sub-millimeter section. The key advantages of this technique lie in the low volume and low-cost of the channel, which makes it suitable to a disposable usage. Fractionation performance and instrumental simplicity make it an interesting methodology for in-batch or on-line pre-MS treatment of such samples.

Original languageEnglish
Title of host publicationDetection of Biological Agents for the Prevention of Bioterrorism
EditorsJoseph Banoub
Pages13-36
Number of pages24
DOIs
Publication statusPublished - 2011 Jan 1

Publication series

NameNATO Science for Peace and Security Series A: Chemistry and Biology
ISSN (Print)1874-6489

Fingerprint

Field Flow Fractionation
Fractionation
Mass spectrometry
Mass Spectrometry
Flow fields
Bacteria
Proteins
Fibers
Molar mass
Membranes
Costs and Cost Analysis
Costs

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Safety, Risk, Reliability and Quality

Cite this

Reschiglian, P., Zattoni, A., Rambaldi, D. C., Roda, A., & Moon, M. H. (2011). Hollow-fiber flow field-flow fractionation for mass spectrometry: From proteins to whole bacteria. In J. Banoub (Ed.), Detection of Biological Agents for the Prevention of Bioterrorism (pp. 13-36). (NATO Science for Peace and Security Series A: Chemistry and Biology). https://doi.org/10.1007/978-90-481-9815-3-2
Reschiglian, Pierluigi ; Zattoni, Andrea ; Rambaldi, Diana Cristina ; Roda, Aldo ; Moon, Myeong Hee. / Hollow-fiber flow field-flow fractionation for mass spectrometry : From proteins to whole bacteria. Detection of Biological Agents for the Prevention of Bioterrorism. editor / Joseph Banoub. 2011. pp. 13-36 (NATO Science for Peace and Security Series A: Chemistry and Biology).
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Reschiglian, P, Zattoni, A, Rambaldi, DC, Roda, A & Moon, MH 2011, Hollow-fiber flow field-flow fractionation for mass spectrometry: From proteins to whole bacteria. in J Banoub (ed.), Detection of Biological Agents for the Prevention of Bioterrorism. NATO Science for Peace and Security Series A: Chemistry and Biology, pp. 13-36. https://doi.org/10.1007/978-90-481-9815-3-2

Hollow-fiber flow field-flow fractionation for mass spectrometry : From proteins to whole bacteria. / Reschiglian, Pierluigi; Zattoni, Andrea; Rambaldi, Diana Cristina; Roda, Aldo; Moon, Myeong Hee.

Detection of Biological Agents for the Prevention of Bioterrorism. ed. / Joseph Banoub. 2011. p. 13-36 (NATO Science for Peace and Security Series A: Chemistry and Biology).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Reschiglian P, Zattoni A, Rambaldi DC, Roda A, Moon MH. Hollow-fiber flow field-flow fractionation for mass spectrometry: From proteins to whole bacteria. In Banoub J, editor, Detection of Biological Agents for the Prevention of Bioterrorism. 2011. p. 13-36. (NATO Science for Peace and Security Series A: Chemistry and Biology). https://doi.org/10.1007/978-90-481-9815-3-2