Abstract
Mass spectrometry (MS) provides analyte identification over a wide molar-mass range. However, particularly in the case of complex matrices, this ability is often enhanced by the use of pre-MS separation steps. A separation, prototype technique for the "gentle" fractionation of large/ultralarge analytes, from proteins to whole cells, is here described to reduce complexity and maintain native characteristics of the sample before MS analysis. It is based on flow field-flow fractionation, and it employs a micro-volume fractionation channel made of a ca. 20 cm hollow-fiber membrane of sub-millimeter section. The key advantages of this technique lie in the low volume and low-cost of the channel, which makes it suitable to a disposable usage. Fractionation performance and instrumental simplicity make it an interesting methodology for in-batch or on-line pre-MS treatment of such samples.
| Original language | English |
|---|---|
| Title of host publication | Detection of Biological Agents for the Prevention of Bioterrorism |
| Editors | Joseph Banoub |
| Pages | 13-36 |
| Number of pages | 24 |
| DOIs | |
| Publication status | Published - 2011 Jan 1 |
Publication series
| Name | NATO Science for Peace and Security Series A: Chemistry and Biology |
|---|---|
| ISSN (Print) | 1874-6489 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Biochemistry, Genetics and Molecular Biology (miscellaneous)
- Safety, Risk, Reliability and Quality
Cite this
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Hollow-fiber flow field-flow fractionation for mass spectrometry : From proteins to whole bacteria. / Reschiglian, Pierluigi; Zattoni, Andrea; Rambaldi, Diana Cristina; Roda, Aldo; Moon, Myeong Hee.
Detection of Biological Agents for the Prevention of Bioterrorism. ed. / Joseph Banoub. 2011. p. 13-36 (NATO Science for Peace and Security Series A: Chemistry and Biology).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Hollow-fiber flow field-flow fractionation for mass spectrometry
T2 - From proteins to whole bacteria
AU - Reschiglian, Pierluigi
AU - Zattoni, Andrea
AU - Rambaldi, Diana Cristina
AU - Roda, Aldo
AU - Moon, Myeong Hee
PY - 2011/1/1
Y1 - 2011/1/1
N2 - Mass spectrometry (MS) provides analyte identification over a wide molar-mass range. However, particularly in the case of complex matrices, this ability is often enhanced by the use of pre-MS separation steps. A separation, prototype technique for the "gentle" fractionation of large/ultralarge analytes, from proteins to whole cells, is here described to reduce complexity and maintain native characteristics of the sample before MS analysis. It is based on flow field-flow fractionation, and it employs a micro-volume fractionation channel made of a ca. 20 cm hollow-fiber membrane of sub-millimeter section. The key advantages of this technique lie in the low volume and low-cost of the channel, which makes it suitable to a disposable usage. Fractionation performance and instrumental simplicity make it an interesting methodology for in-batch or on-line pre-MS treatment of such samples.
AB - Mass spectrometry (MS) provides analyte identification over a wide molar-mass range. However, particularly in the case of complex matrices, this ability is often enhanced by the use of pre-MS separation steps. A separation, prototype technique for the "gentle" fractionation of large/ultralarge analytes, from proteins to whole cells, is here described to reduce complexity and maintain native characteristics of the sample before MS analysis. It is based on flow field-flow fractionation, and it employs a micro-volume fractionation channel made of a ca. 20 cm hollow-fiber membrane of sub-millimeter section. The key advantages of this technique lie in the low volume and low-cost of the channel, which makes it suitable to a disposable usage. Fractionation performance and instrumental simplicity make it an interesting methodology for in-batch or on-line pre-MS treatment of such samples.
UR - http://www.scopus.com/inward/record.url?scp=78650505154&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650505154&partnerID=8YFLogxK
U2 - 10.1007/978-90-481-9815-3-2
DO - 10.1007/978-90-481-9815-3-2
M3 - Conference contribution
AN - SCOPUS:78650505154
SN - 9789048198146
T3 - NATO Science for Peace and Security Series A: Chemistry and Biology
SP - 13
EP - 36
BT - Detection of Biological Agents for the Prevention of Bioterrorism
A2 - Banoub, Joseph
ER -