Hollow-fiber flow field-flow fractionation of whole blood serum

Andrea Zattoni, Diana Cristina Rambaldi, Barbara Roda, Daniela Parisi, Aldo Roda, Myeong Hee Moon, Pierluigi Reschiglian

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Hollow-fiber flow field-flow fractionation is here applied to untreated, whole human blood serum. Matrix-assisted, laser desorption/ionization time-of-flight-mass spectrometry (MALDI-TOF-MS) of serum fractions shows mass signals in the <30 000 Mr range where low-abundance, serum protein components are known to be present, though a membrane of nominal 30 000 Da cutoff was employed for the fractionation device. Using diluted sera spiked with low amounts (0.06-0.1%, w/w) of an artificial mixture constituted the human adrenocorticotropic hormone fragments 18-39 (Mr = 2465.7) and 7-38 (Mr = 3659.2), and of bovine insulin (Mr = 5734), horse cytochrome c (Mr = 12 384) and chicken lysozyme (Mr = 14 388), a hybrid fractionation/microfiltration mechanism shows to govern the separation of the low-Mr components.

Original languageEnglish
Pages (from-to)135-142
Number of pages8
JournalJournal of Chromatography A
Volume1183
Issue number1-2
DOIs
Publication statusPublished - 2008 Mar 7

Fingerprint

Field Flow Fractionation
Fractionation
Flow fields
Blood
Fibers
Serum
Microfiltration
Muramidase
Cytochromes c
Adrenocorticotropic Hormone
Horses
Ionization
Mass spectrometry
Blood Proteins
Chickens
Desorption
Mass Spectrometry
Lasers
Insulin
Membranes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Zattoni, A., Rambaldi, D. C., Roda, B., Parisi, D., Roda, A., Moon, M. H., & Reschiglian, P. (2008). Hollow-fiber flow field-flow fractionation of whole blood serum. Journal of Chromatography A, 1183(1-2), 135-142. https://doi.org/10.1016/j.chroma.2008.01.022
Zattoni, Andrea ; Rambaldi, Diana Cristina ; Roda, Barbara ; Parisi, Daniela ; Roda, Aldo ; Moon, Myeong Hee ; Reschiglian, Pierluigi. / Hollow-fiber flow field-flow fractionation of whole blood serum. In: Journal of Chromatography A. 2008 ; Vol. 1183, No. 1-2. pp. 135-142.
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Zattoni, A, Rambaldi, DC, Roda, B, Parisi, D, Roda, A, Moon, MH & Reschiglian, P 2008, 'Hollow-fiber flow field-flow fractionation of whole blood serum', Journal of Chromatography A, vol. 1183, no. 1-2, pp. 135-142. https://doi.org/10.1016/j.chroma.2008.01.022

Hollow-fiber flow field-flow fractionation of whole blood serum. / Zattoni, Andrea; Rambaldi, Diana Cristina; Roda, Barbara; Parisi, Daniela; Roda, Aldo; Moon, Myeong Hee; Reschiglian, Pierluigi.

In: Journal of Chromatography A, Vol. 1183, No. 1-2, 07.03.2008, p. 135-142.

Research output: Contribution to journalArticle

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AU - Moon, Myeong Hee

AU - Reschiglian, Pierluigi

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