Hollow-fiber flow field-flow fractionation of whole blood serum

Andrea Zattoni, Diana Cristina Rambaldi, Barbara Roda, Daniela Parisi, Aldo Roda, Myeong Hee Moon, Pierluigi Reschiglian

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Abstract

Hollow-fiber flow field-flow fractionation is here applied to untreated, whole human blood serum. Matrix-assisted, laser desorption/ionization time-of-flight-mass spectrometry (MALDI-TOF-MS) of serum fractions shows mass signals in the <30 000 Mr range where low-abundance, serum protein components are known to be present, though a membrane of nominal 30 000 Da cutoff was employed for the fractionation device. Using diluted sera spiked with low amounts (0.06-0.1%, w/w) of an artificial mixture constituted the human adrenocorticotropic hormone fragments 18-39 (Mr = 2465.7) and 7-38 (Mr = 3659.2), and of bovine insulin (Mr = 5734), horse cytochrome c (Mr = 12 384) and chicken lysozyme (Mr = 14 388), a hybrid fractionation/microfiltration mechanism shows to govern the separation of the low-Mr components.

Original languageEnglish
Pages (from-to)135-142
Number of pages8
JournalJournal of Chromatography A
Volume1183
Issue number1-2
DOIs
Publication statusPublished - 2008 Mar 7

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Zattoni, A., Rambaldi, D. C., Roda, B., Parisi, D., Roda, A., Moon, M. H., & Reschiglian, P. (2008). Hollow-fiber flow field-flow fractionation of whole blood serum. Journal of Chromatography A, 1183(1-2), 135-142. https://doi.org/10.1016/j.chroma.2008.01.022