Hollow-fiber flow field-flow fractionation of whole blood serum

Andrea Zattoni; Diana Cristina Rambaldi; Barbara Roda; Daniela Parisi; Aldo Roda; Myeong Hee Moon; Pierluigi Reschiglian

doi:10.1016/j.chroma.2008.01.022

Hollow-fiber flow field-flow fractionation of whole blood serum

Andrea Zattoni, Diana Cristina Rambaldi, Barbara Roda, Daniela Parisi, Aldo Roda, Myeong Hee Moon, Pierluigi Reschiglian

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Hollow-fiber flow field-flow fractionation is here applied to untreated, whole human blood serum. Matrix-assisted, laser desorption/ionization time-of-flight-mass spectrometry (MALDI-TOF-MS) of serum fractions shows mass signals in the <30 000 M_r range where low-abundance, serum protein components are known to be present, though a membrane of nominal 30 000 Da cutoff was employed for the fractionation device. Using diluted sera spiked with low amounts (0.06-0.1%, w/w) of an artificial mixture constituted the human adrenocorticotropic hormone fragments 18-39 (M_r = 2465.7) and 7-38 (M_r = 3659.2), and of bovine insulin (M_r = 5734), horse cytochrome c (M_r = 12 384) and chicken lysozyme (M_r = 14 388), a hybrid fractionation/microfiltration mechanism shows to govern the separation of the low-M_r components.

Original language	English
Pages (from-to)	135-142
Number of pages	8
Journal	Journal of Chromatography A
Volume	1183
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chroma.2008.01.022
Publication status	Published - 2008 Mar 7

Bibliographical note

Funding Information:
The work was partially supported by the University of Bologna (funds for selected research topics), and by the Italian Ministry of the University and Research (MiUR, PRIN 2006, 2006033944). P.R. and M.-H.M. duly acknowledge the Significant Bilateral Project “Novel Methods for Top-down Analysis of Macromolecular and Nanosized Samples of Biotechnological and Environmental Interest” within the VIII Executive Programme of Scientific and Technological Co-operation between Italy and Korea for the years 2007–2009.

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/j.chroma.2008.01.022

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abstract = "Hollow-fiber flow field-flow fractionation is here applied to untreated, whole human blood serum. Matrix-assisted, laser desorption/ionization time-of-flight-mass spectrometry (MALDI-TOF-MS) of serum fractions shows mass signals in the <30 000 Mr range where low-abundance, serum protein components are known to be present, though a membrane of nominal 30 000 Da cutoff was employed for the fractionation device. Using diluted sera spiked with low amounts (0.06-0.1%, w/w) of an artificial mixture constituted the human adrenocorticotropic hormone fragments 18-39 (Mr = 2465.7) and 7-38 (Mr = 3659.2), and of bovine insulin (Mr = 5734), horse cytochrome c (Mr = 12 384) and chicken lysozyme (Mr = 14 388), a hybrid fractionation/microfiltration mechanism shows to govern the separation of the low-Mr components.",

author = "Andrea Zattoni and Rambaldi, {Diana Cristina} and Barbara Roda and Daniela Parisi and Aldo Roda and Moon, {Myeong Hee} and Pierluigi Reschiglian",

note = "Funding Information: The work was partially supported by the University of Bologna (funds for selected research topics), and by the Italian Ministry of the University and Research (MiUR, PRIN 2006, 2006033944). P.R. and M.-H.M. duly acknowledge the Significant Bilateral Project “Novel Methods for Top-down Analysis of Macromolecular and Nanosized Samples of Biotechnological and Environmental Interest” within the VIII Executive Programme of Scientific and Technological Co-operation between Italy and Korea for the years 2007–2009. ",

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AU - Zattoni, Andrea

AU - Rambaldi, Diana Cristina

AU - Roda, Barbara

AU - Parisi, Daniela

AU - Roda, Aldo

AU - Moon, Myeong Hee

AU - Reschiglian, Pierluigi

N1 - Funding Information: The work was partially supported by the University of Bologna (funds for selected research topics), and by the Italian Ministry of the University and Research (MiUR, PRIN 2006, 2006033944). P.R. and M.-H.M. duly acknowledge the Significant Bilateral Project “Novel Methods for Top-down Analysis of Macromolecular and Nanosized Samples of Biotechnological and Environmental Interest” within the VIII Executive Programme of Scientific and Technological Co-operation between Italy and Korea for the years 2007–2009.

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N2 - Hollow-fiber flow field-flow fractionation is here applied to untreated, whole human blood serum. Matrix-assisted, laser desorption/ionization time-of-flight-mass spectrometry (MALDI-TOF-MS) of serum fractions shows mass signals in the <30 000 Mr range where low-abundance, serum protein components are known to be present, though a membrane of nominal 30 000 Da cutoff was employed for the fractionation device. Using diluted sera spiked with low amounts (0.06-0.1%, w/w) of an artificial mixture constituted the human adrenocorticotropic hormone fragments 18-39 (Mr = 2465.7) and 7-38 (Mr = 3659.2), and of bovine insulin (Mr = 5734), horse cytochrome c (Mr = 12 384) and chicken lysozyme (Mr = 14 388), a hybrid fractionation/microfiltration mechanism shows to govern the separation of the low-Mr components.

AB - Hollow-fiber flow field-flow fractionation is here applied to untreated, whole human blood serum. Matrix-assisted, laser desorption/ionization time-of-flight-mass spectrometry (MALDI-TOF-MS) of serum fractions shows mass signals in the <30 000 Mr range where low-abundance, serum protein components are known to be present, though a membrane of nominal 30 000 Da cutoff was employed for the fractionation device. Using diluted sera spiked with low amounts (0.06-0.1%, w/w) of an artificial mixture constituted the human adrenocorticotropic hormone fragments 18-39 (Mr = 2465.7) and 7-38 (Mr = 3659.2), and of bovine insulin (Mr = 5734), horse cytochrome c (Mr = 12 384) and chicken lysozyme (Mr = 14 388), a hybrid fractionation/microfiltration mechanism shows to govern the separation of the low-Mr components.

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Hollow-fiber flow field-flow fractionation of whole blood serum

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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