Holographic approach for optical poration and trapping of developing embryos

Maria Leilani Torres-Mapa, Maciej Antkowiak, Hana Cizmarova, David E.K. Ferrier, Kishan Dholakia, Frank Gunn-Moore

Research output: Contribution to conferencePaper

Abstract

Optical techniques such as poration and trapping can be viable tools toward micromanipulation of living organisms. In embryos, there are two major technical issues that need to be addressed. Injection of fluorophores or genetic constructs into a single cell of an embryo will enable cell-fate mapping and determine timing dependent protein functionality during its development. Furthermore, a non-contact method for orienting the embryo at its later stage of development is desirable for high magnification imaging. In this work, we present a holographic based system that can be utilized for both approaches as shown in our previous work [1]. Using a pulsed Ti:Sapphire femtosecond (fs) laser and a spatial light modulator (SLM), we can demonstrate successful injection of impermeable molecules such as fluorescently labeled dextran molecules and Propidium iodide into Pomatoceros lamarckii embryos. By using the continuous wave output of a Ti:Sapphire laser, we can perform optical trapping of embryos using low numerical aperture (NA) objectives.

Original languageEnglish
DOIs
Publication statusPublished - 2013 Jan 1
Event2013 Conference on Lasers and Electro-Optics Europe and International Quantum Electronics Conference, CLEO/Europe-IQEC 2013 - Munich, Germany
Duration: 2013 May 122013 May 16

Other

Other2013 Conference on Lasers and Electro-Optics Europe and International Quantum Electronics Conference, CLEO/Europe-IQEC 2013
CountryGermany
CityMunich
Period13/5/1213/5/16

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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    Torres-Mapa, M. L., Antkowiak, M., Cizmarova, H., Ferrier, D. E. K., Dholakia, K., & Gunn-Moore, F. (2013). Holographic approach for optical poration and trapping of developing embryos. Paper presented at 2013 Conference on Lasers and Electro-Optics Europe and International Quantum Electronics Conference, CLEO/Europe-IQEC 2013, Munich, Germany. https://doi.org/10.1109/CLEOE-IQEC.2013.6801494