Homeobox D1 regulates angiogenic functions of endothelial cells via integrin β1 expression

Hyojin Park; Hyun Jung Choi; Jihye Kim; Minhyung Kim; Seung Sik Rho; Daehee Hwang; Young Myeong Kim; Young Guen Kwon

doi:10.1016/j.bbrc.2011.04.017

Homeobox D1 regulates angiogenic functions of endothelial cells via integrin β1 expression

Hyojin Park, Hyun Jung Choi, Jihye Kim, Minhyung Kim, Seung Sik Rho, Daehee Hwang, Young Myeong Kim, Young Guen Kwon

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Homeobox (HOX) family genes, major transcription factors for embryonic development, have been also implicated in vascular development and angiogenesis, particularly with regulation of genes involved in cell-cell or cell-extracellular matrix (ECM) interactions. However, the cellular and molecular functions of HOXD1 in endothelial cells (ECs) are yet to be explored. We here report that HOXD1 is prominently expressed in human ECs and regulates angiogenic activities. Knockdown of HOXD1 in ECs resulted in significant inhibition of migration and adhesion as well as tube like structure formation. These effects were correlated with the reduced expression of integrin β1 (ITGB1), an important signaling component of angiogenesis. Consistently, ITGB1 promoter activity was decreased by HOXD1 knockdown in ECs. Furthermore, we identified the putative HOXD1-binding sites in the promoter region of ITGB1. Together, these findings suggest that HOXD1 plays a significant role in EC functions by regulating the expression of ITGB1.

Original language	English
Pages (from-to)	186-192
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	408
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2011.04.017
Publication status	Published - 2011 Apr 29

Bibliographical note

Funding Information:
This work was supported by a National Research Laboratory and Science Technology Grant (20090083119) and a grant from the Korea Health 21 R&D Project, Ministry of Health Welfare & Family Affairs (A085136), Korea.

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Homeobox D1 regulates angiogenic functions of endothelial cells via integrin β1 expression",

abstract = "Homeobox (HOX) family genes, major transcription factors for embryonic development, have been also implicated in vascular development and angiogenesis, particularly with regulation of genes involved in cell-cell or cell-extracellular matrix (ECM) interactions. However, the cellular and molecular functions of HOXD1 in endothelial cells (ECs) are yet to be explored. We here report that HOXD1 is prominently expressed in human ECs and regulates angiogenic activities. Knockdown of HOXD1 in ECs resulted in significant inhibition of migration and adhesion as well as tube like structure formation. These effects were correlated with the reduced expression of integrin β1 (ITGB1), an important signaling component of angiogenesis. Consistently, ITGB1 promoter activity was decreased by HOXD1 knockdown in ECs. Furthermore, we identified the putative HOXD1-binding sites in the promoter region of ITGB1. Together, these findings suggest that HOXD1 plays a significant role in EC functions by regulating the expression of ITGB1.",

author = "Hyojin Park and Choi, {Hyun Jung} and Jihye Kim and Minhyung Kim and Rho, {Seung Sik} and Daehee Hwang and Kim, {Young Myeong} and Kwon, {Young Guen}",

note = "Funding Information: This work was supported by a National Research Laboratory and Science Technology Grant (20090083119) and a grant from the Korea Health 21 R&D Project, Ministry of Health Welfare & Family Affairs (A085136), Korea. ",

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Homeobox D1 regulates angiogenic functions of endothelial cells via integrin β1 expression. / Park, Hyojin; Choi, Hyun Jung; Kim, Jihye; Kim, Minhyung; Rho, Seung Sik; Hwang, Daehee; Kim, Young Myeong; Kwon, Young Guen.

In: Biochemical and Biophysical Research Communications, Vol. 408, No. 1, 29.04.2011, p. 186-192.

Research output: Contribution to journal › Article › peer-review

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AU - Rho, Seung Sik

AU - Hwang, Daehee

AU - Kim, Young Myeong

AU - Kwon, Young Guen

N1 - Funding Information: This work was supported by a National Research Laboratory and Science Technology Grant (20090083119) and a grant from the Korea Health 21 R&D Project, Ministry of Health Welfare & Family Affairs (A085136), Korea.

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N2 - Homeobox (HOX) family genes, major transcription factors for embryonic development, have been also implicated in vascular development and angiogenesis, particularly with regulation of genes involved in cell-cell or cell-extracellular matrix (ECM) interactions. However, the cellular and molecular functions of HOXD1 in endothelial cells (ECs) are yet to be explored. We here report that HOXD1 is prominently expressed in human ECs and regulates angiogenic activities. Knockdown of HOXD1 in ECs resulted in significant inhibition of migration and adhesion as well as tube like structure formation. These effects were correlated with the reduced expression of integrin β1 (ITGB1), an important signaling component of angiogenesis. Consistently, ITGB1 promoter activity was decreased by HOXD1 knockdown in ECs. Furthermore, we identified the putative HOXD1-binding sites in the promoter region of ITGB1. Together, these findings suggest that HOXD1 plays a significant role in EC functions by regulating the expression of ITGB1.

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Homeobox D1 regulates angiogenic functions of endothelial cells via integrin β1 expression

Abstract

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