Homologous alkalophilic and acidophilic L-arabinose isomerases reveal region-specific contributions to the ph dependence of activity and stability

Sang Jae Lee; Sang Jun Lee; Yong Jik Lee; Seong Bo Kim; Sung Kun Kim; Dong Woo Lee

doi:10.1128/AEM.02114-12

Homologous alkalophilic and acidophilic L-arabinose isomerases reveal region-specific contributions to the ph dependence of activity and stability

Sang Jae Lee, Sang Jun Lee, Yong Jik Lee, Seong Bo Kim, Sung Kun Kim, Dong Woo Lee

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

To study the pH dependence of L-arabinose isomerase (AI) activity and stability, we compared homologous AIs with their chimeras. This study demonstrated that an ionizable amino acid near the catalytic site determines the optimal pH (pH_opt) for activity, whereas the N-terminal surface R residues play an important role in determining the pH_opt for stability.

Original language	English
Pages (from-to)	8813-8816
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	78
Issue number	24
DOIs	https://doi.org/10.1128/AEM.02114-12
Publication status	Published - 2012 Dec

All Science Journal Classification (ASJC) codes

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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abstract = "To study the pH dependence of L-arabinose isomerase (AI) activity and stability, we compared homologous AIs with their chimeras. This study demonstrated that an ionizable amino acid near the catalytic site determines the optimal pH (pHopt) for activity, whereas the N-terminal surface R residues play an important role in determining the pHopt for stability.",

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AU - Lee, Sang Jun

AU - Lee, Yong Jik

AU - Kim, Seong Bo

AU - Kim, Sung Kun

AU - Lee, Dong Woo

PY - 2012/12

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AB - To study the pH dependence of L-arabinose isomerase (AI) activity and stability, we compared homologous AIs with their chimeras. This study demonstrated that an ionizable amino acid near the catalytic site determines the optimal pH (pHopt) for activity, whereas the N-terminal surface R residues play an important role in determining the pHopt for stability.

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Homologous alkalophilic and acidophilic L-arabinose isomerases reveal region-specific contributions to the ph dependence of activity and stability

Abstract

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