Homologous alkalophilic and acidophilic L-arabinose isomerases reveal region-specific contributions to the ph dependence of activity and stability

Sang Jae Lee, Sang Jun Lee, Yong Jik Lee, Seong Bo Kim, Sung Kun Kim, Dong Woo Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To study the pH dependence of L-arabinose isomerase (AI) activity and stability, we compared homologous AIs with their chimeras. This study demonstrated that an ionizable amino acid near the catalytic site determines the optimal pH (pHopt) for activity, whereas the N-terminal surface R residues play an important role in determining the pHopt for stability.

Original languageEnglish
Pages (from-to)8813-8816
Number of pages4
JournalApplied and Environmental Microbiology
Volume78
Issue number24
DOIs
Publication statusPublished - 2012 Dec 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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