Homozygous deletions of the CDKN2 gene and loss of heterozygosity of 9p in primary hepatocellular carcinoma

Zhe Piao, Chanil Park, Jin Sung Lee, Chang Hyun Yang, Kang Yell Choi, Hoguen Kim

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

To elucidate the alterations of CDKN2 in hepatocarcinogenesis, we performed a loss of heterozygosity (LOH) study using eight polymorphic markers surrounding the CDKN2 gene and analyzed the homozygous deletions and mutations of the CDKN2 gene in 41 primary hepatocellular carcinomas (HCCs). Frequent LOH (27.8-44%) was found in the eight loci on chromosome 9p, however, no intragenic mutations of CDKN2 were observed by PCR-SSCP analysis. Homozygous deletions were detected in 25 of 41 HCCs (61%) by a comparative multiplex PCR. No expression of the CDKN2 protein was noted in five out of nine available HCCs by Western blot analysis. These results suggest that inactivation of the CDKN2 gene in HCC is a frequent event in which homozygous deletions are the most common mechanism of CDKN2 inactivation.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalCancer Letters
Volume122
Issue number1-2
DOIs
Publication statusPublished - 1998 Jan 9

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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