Abstract
Highly pathogenic avian influenza virus (HPAIV) infections have occurred continuously and crossed the species barrier to humans, leading to fatalities. A polymerase chain reaction based molecular test is currently the most sensitive diagnostic tool for HPAIV; however, the results must be analyzed in centralized diagnosis systems by a trained individual. This requirement leads to delays in quarantine and isolation. To control the spread of HPAIV, rapid and accurate diagnostics suitable for field testing are needed, and the tests must facilitate a differential diagnosis between HPAIV and low pathogenic avian influenza virus (LPAIV), which undergo cleavage specifically by trypsin- or furin-like proteases, respectively. In this study, a differential avian influenza virus rapid test kit is developed and evaluated in vitro and using clinical specimens from HPAIV H5N1-infected animals. It is demonstrated that this rapid test kit provides highly sensitive and specific detection of HPAIV and LPAIV and is thus a useful field diagnostic tool for H5N1 HPAIV outbreaks and for rapid quarantine control of the disease.
| Original language | English |
|---|---|
| Article number | 1800960 |
| Journal | Advanced Functional Materials |
| Volume | 28 |
| Issue number | 34 |
| DOIs | |
| Publication status | Published - 2018 Aug 22 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Chemistry(all)
- Materials Science(all)
- Condensed Matter Physics
- Electrochemistry
Cite this
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Host Cell Mimic Polymersomes for Rapid Detection of Highly Pathogenic Influenza Virus via a Viral Fusion and Cell Entry Mechanism. / Kim, Hyun Ouk; Na, Woonsung; Yeom, Minjoo; Choi, Jihye; Kim, Jihye; Lim, Jong Woo; Yun, Dayeon; Chun, Haejin; Park, Geunseon; Park, Chaewon; Kim, Jeong Ki; Jeong, Dae Gwin; Le, Van Phan; Lee, Kwangyeol; Lee, Jae Myun; Jeong, Hyoung Hwa; Song, Daesub; Haam, Seungjoo.
In: Advanced Functional Materials, Vol. 28, No. 34, 1800960, 22.08.2018.Research output: Contribution to journal › Article
TY - JOUR
T1 - Host Cell Mimic Polymersomes for Rapid Detection of Highly Pathogenic Influenza Virus via a Viral Fusion and Cell Entry Mechanism
AU - Kim, Hyun Ouk
AU - Na, Woonsung
AU - Yeom, Minjoo
AU - Choi, Jihye
AU - Kim, Jihye
AU - Lim, Jong Woo
AU - Yun, Dayeon
AU - Chun, Haejin
AU - Park, Geunseon
AU - Park, Chaewon
AU - Kim, Jeong Ki
AU - Jeong, Dae Gwin
AU - Le, Van Phan
AU - Lee, Kwangyeol
AU - Lee, Jae Myun
AU - Jeong, Hyoung Hwa
AU - Song, Daesub
AU - Haam, Seungjoo
PY - 2018/8/22
Y1 - 2018/8/22
N2 - Highly pathogenic avian influenza virus (HPAIV) infections have occurred continuously and crossed the species barrier to humans, leading to fatalities. A polymerase chain reaction based molecular test is currently the most sensitive diagnostic tool for HPAIV; however, the results must be analyzed in centralized diagnosis systems by a trained individual. This requirement leads to delays in quarantine and isolation. To control the spread of HPAIV, rapid and accurate diagnostics suitable for field testing are needed, and the tests must facilitate a differential diagnosis between HPAIV and low pathogenic avian influenza virus (LPAIV), which undergo cleavage specifically by trypsin- or furin-like proteases, respectively. In this study, a differential avian influenza virus rapid test kit is developed and evaluated in vitro and using clinical specimens from HPAIV H5N1-infected animals. It is demonstrated that this rapid test kit provides highly sensitive and specific detection of HPAIV and LPAIV and is thus a useful field diagnostic tool for H5N1 HPAIV outbreaks and for rapid quarantine control of the disease.
AB - Highly pathogenic avian influenza virus (HPAIV) infections have occurred continuously and crossed the species barrier to humans, leading to fatalities. A polymerase chain reaction based molecular test is currently the most sensitive diagnostic tool for HPAIV; however, the results must be analyzed in centralized diagnosis systems by a trained individual. This requirement leads to delays in quarantine and isolation. To control the spread of HPAIV, rapid and accurate diagnostics suitable for field testing are needed, and the tests must facilitate a differential diagnosis between HPAIV and low pathogenic avian influenza virus (LPAIV), which undergo cleavage specifically by trypsin- or furin-like proteases, respectively. In this study, a differential avian influenza virus rapid test kit is developed and evaluated in vitro and using clinical specimens from HPAIV H5N1-infected animals. It is demonstrated that this rapid test kit provides highly sensitive and specific detection of HPAIV and LPAIV and is thus a useful field diagnostic tool for H5N1 HPAIV outbreaks and for rapid quarantine control of the disease.
UR - http://www.scopus.com/inward/record.url?scp=85051734175&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85051734175&partnerID=8YFLogxK
U2 - 10.1002/adfm.201800960
DO - 10.1002/adfm.201800960
M3 - Article
AN - SCOPUS:85051734175
VL - 28
JO - Advanced Functional Materials
JF - Advanced Functional Materials
SN - 1616-301X
IS - 34
M1 - 1800960
ER -