Host immune responses to antigens derived from a predominant strain of Mycobacterium tuberculosis

Yun Gyoung Hur, Wou Young Chung, Ahreum Kim, Young Sun Kim, Hyonsuk Kim, Sun Hee Jang, Yeun Kim, Hyeyoung Lee, Kwang Joo Park, Sangnae Cho

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objectives: Beijing strain of Mycobacterium tuberculosis (M. tb) is characterized by a high incidence of transmission, relapse, and drug resistance. This study aimed to determine host immune responses to antigens derived from the Beijing/K strain which has been highly prevalent in tuberculosis (TB) outbreaks in South Korea. Methods: We recruited 52 TB patients and 96 healthy subjects comprising 31 individuals with latent TB infection (LTBI) and 65 TB-naïve controls based on QuantiFERON-TB Gold In-Tube (QFT-IT) tests. Blood samples were obtained for diluted whole-blood assays, multiplex bead arrays, ELISpot assays, and HLA typing. Molecular genotyping of M. tb was performed using clinical isolates. Results: Active TB and LTBI groups were differentiated by TNF-α concentrations induced by the Beijing/K strain-derived antigens, MTBK_24790 and MTBK_24800 (P < 0.001). MTBK_24800-induced IFN-γ and CXCL10 concentrations discriminated the TB-infected groups from TB-naïve controls (P < 0.001). IFN-γ-producing T cells were generated in 87.2% of TB patients in response to MTBK_24800 peptide antigens. The major immunogenic epitope was at C-terminal of the antigen, and predominantly recognized by HLA-DR4 and -DQ4. Conclusions: Measurement of IFN-γ, CXCL10, and TNF-α concentrations induced by MTBK_24790 and MTBK_24800 may contribute to improved diagnosis of TB and vaccine development in regions where the Beijing/K strain is endemic.

Original languageEnglish
Pages (from-to)54-62
Number of pages9
JournalJournal of Infection
Volume73
Issue number1
DOIs
Publication statusPublished - 2016 Jul 1

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Histocompatibility Antigens Class II
Mycobacterium tuberculosis
Tuberculosis
Latent Tuberculosis
Antigens
Tuberculosis Vaccines
HLA-DR4 Antigen
Histocompatibility Testing
Republic of Korea
Drug Resistance
Gold
Disease Outbreaks
Epitopes
Healthy Volunteers
T-Lymphocytes
Recurrence
Peptides
Beijing
Incidence

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Hur, Yun Gyoung ; Chung, Wou Young ; Kim, Ahreum ; Kim, Young Sun ; Kim, Hyonsuk ; Jang, Sun Hee ; Kim, Yeun ; Lee, Hyeyoung ; Park, Kwang Joo ; Cho, Sangnae. / Host immune responses to antigens derived from a predominant strain of Mycobacterium tuberculosis. In: Journal of Infection. 2016 ; Vol. 73, No. 1. pp. 54-62.
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abstract = "Objectives: Beijing strain of Mycobacterium tuberculosis (M. tb) is characterized by a high incidence of transmission, relapse, and drug resistance. This study aimed to determine host immune responses to antigens derived from the Beijing/K strain which has been highly prevalent in tuberculosis (TB) outbreaks in South Korea. Methods: We recruited 52 TB patients and 96 healthy subjects comprising 31 individuals with latent TB infection (LTBI) and 65 TB-na{\"i}ve controls based on QuantiFERON-TB Gold In-Tube (QFT-IT) tests. Blood samples were obtained for diluted whole-blood assays, multiplex bead arrays, ELISpot assays, and HLA typing. Molecular genotyping of M. tb was performed using clinical isolates. Results: Active TB and LTBI groups were differentiated by TNF-α concentrations induced by the Beijing/K strain-derived antigens, MTBK_24790 and MTBK_24800 (P < 0.001). MTBK_24800-induced IFN-γ and CXCL10 concentrations discriminated the TB-infected groups from TB-na{\"i}ve controls (P < 0.001). IFN-γ-producing T cells were generated in 87.2{\%} of TB patients in response to MTBK_24800 peptide antigens. The major immunogenic epitope was at C-terminal of the antigen, and predominantly recognized by HLA-DR4 and -DQ4. Conclusions: Measurement of IFN-γ, CXCL10, and TNF-α concentrations induced by MTBK_24790 and MTBK_24800 may contribute to improved diagnosis of TB and vaccine development in regions where the Beijing/K strain is endemic.",
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Host immune responses to antigens derived from a predominant strain of Mycobacterium tuberculosis. / Hur, Yun Gyoung; Chung, Wou Young; Kim, Ahreum; Kim, Young Sun; Kim, Hyonsuk; Jang, Sun Hee; Kim, Yeun; Lee, Hyeyoung; Park, Kwang Joo; Cho, Sangnae.

In: Journal of Infection, Vol. 73, No. 1, 01.07.2016, p. 54-62.

Research output: Contribution to journalArticle

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AU - Hur, Yun Gyoung

AU - Chung, Wou Young

AU - Kim, Ahreum

AU - Kim, Young Sun

AU - Kim, Hyonsuk

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AU - Park, Kwang Joo

AU - Cho, Sangnae

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