House dust mite allergen Der f 2-induced phospholipase D1 activation is critical for the production of interleukin-13 through activating transcription factor-2 activation in human bronchial epithelial cells

Shin Young Park, Ju Hwan Cho, Doo Yi Oh, Jung Won Park, Myung Ju Ahn, Joong Soo Han, Jae Won Oh

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The purpose of this study was to identify the role of phospholipase D1 (PLD1) inDer f2-induced interleukin (IL)-13 production. The major house dust mite allergen, Der f2, increased PLD activity in human bronchial epithelial cells (BEAS-2B), and dominant negative PLD1 or PLD1 siRNA decreased Der f2-induced IL-13 expression and production. Treatment of Der f2 activated the phospholipase Cγ (PLCγ)/protein kinase Cα (PKCα)/p38 MAPK pathway. Der f2-induced PLD activation was attenuated by PLCγ inhibitors (U73122 and PAO), PKCα inhibitors (RO320432 and GO6976), and p38 MAPK inhibitors (SB203580 and SB202190). These results indicate that PLCγ, PKCα, and p38 MAPK act as upstream activators of PLD in Der f2-treated BEAS-2B cells. Furthermore, expression and production of IL-13 increased by Der f2 were also blocked by inhibition of PLCγ, PKCα, or p38 MAPK, indicating that IL-13 expression and production are related to a PLCγ/PKCα/p38 MAPK pathway. We found that activating transcription factor-2 (ATF-2) was activated by Der f2 in BEAS-2B cells and activation of ATF-2 was controlled by PLD1. When ATF-2 activity was blocked with ATF-2 siRNA, Der f2-induced IL-13 expression and production were decreased. Thus, ATF-2 might be one of the transcriptional factors for the expression of IL-13 in Der f2-treated BEAS-2B cells. Taken together, PLD1 acts as an important regulator in Der f2-induced expression and production of IL-13 through activation of ATF-2 in BEAS-2B cells.

Original languageEnglish
Pages (from-to)20099-20110
Number of pages12
JournalJournal of Biological Chemistry
Volume284
Issue number30
DOIs
Publication statusPublished - 2009 Jul 24

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Activating Transcription Factor 2
Dermatophagoides Antigens
Interleukin-13
Type C Phospholipases
p38 Mitogen-Activated Protein Kinases
Epithelial Cells
Chemical activation
Protein Kinase C
Small Interfering RNA
Protein C Inhibitor
Protein Kinase Inhibitors
Dermatophagoides farinae antigen f 2
phospholipase D1
Human Activities

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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title = "House dust mite allergen Der f 2-induced phospholipase D1 activation is critical for the production of interleukin-13 through activating transcription factor-2 activation in human bronchial epithelial cells",
abstract = "The purpose of this study was to identify the role of phospholipase D1 (PLD1) inDer f2-induced interleukin (IL)-13 production. The major house dust mite allergen, Der f2, increased PLD activity in human bronchial epithelial cells (BEAS-2B), and dominant negative PLD1 or PLD1 siRNA decreased Der f2-induced IL-13 expression and production. Treatment of Der f2 activated the phospholipase Cγ (PLCγ)/protein kinase Cα (PKCα)/p38 MAPK pathway. Der f2-induced PLD activation was attenuated by PLCγ inhibitors (U73122 and PAO), PKCα inhibitors (RO320432 and GO6976), and p38 MAPK inhibitors (SB203580 and SB202190). These results indicate that PLCγ, PKCα, and p38 MAPK act as upstream activators of PLD in Der f2-treated BEAS-2B cells. Furthermore, expression and production of IL-13 increased by Der f2 were also blocked by inhibition of PLCγ, PKCα, or p38 MAPK, indicating that IL-13 expression and production are related to a PLCγ/PKCα/p38 MAPK pathway. We found that activating transcription factor-2 (ATF-2) was activated by Der f2 in BEAS-2B cells and activation of ATF-2 was controlled by PLD1. When ATF-2 activity was blocked with ATF-2 siRNA, Der f2-induced IL-13 expression and production were decreased. Thus, ATF-2 might be one of the transcriptional factors for the expression of IL-13 in Der f2-treated BEAS-2B cells. Taken together, PLD1 acts as an important regulator in Der f2-induced expression and production of IL-13 through activation of ATF-2 in BEAS-2B cells.",
author = "Park, {Shin Young} and Cho, {Ju Hwan} and Oh, {Doo Yi} and Park, {Jung Won} and Ahn, {Myung Ju} and Han, {Joong Soo} and Oh, {Jae Won}",
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House dust mite allergen Der f 2-induced phospholipase D1 activation is critical for the production of interleukin-13 through activating transcription factor-2 activation in human bronchial epithelial cells. / Park, Shin Young; Cho, Ju Hwan; Oh, Doo Yi; Park, Jung Won; Ahn, Myung Ju; Han, Joong Soo; Oh, Jae Won.

In: Journal of Biological Chemistry, Vol. 284, No. 30, 24.07.2009, p. 20099-20110.

Research output: Contribution to journalArticle

TY - JOUR

T1 - House dust mite allergen Der f 2-induced phospholipase D1 activation is critical for the production of interleukin-13 through activating transcription factor-2 activation in human bronchial epithelial cells

AU - Park, Shin Young

AU - Cho, Ju Hwan

AU - Oh, Doo Yi

AU - Park, Jung Won

AU - Ahn, Myung Ju

AU - Han, Joong Soo

AU - Oh, Jae Won

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N2 - The purpose of this study was to identify the role of phospholipase D1 (PLD1) inDer f2-induced interleukin (IL)-13 production. The major house dust mite allergen, Der f2, increased PLD activity in human bronchial epithelial cells (BEAS-2B), and dominant negative PLD1 or PLD1 siRNA decreased Der f2-induced IL-13 expression and production. Treatment of Der f2 activated the phospholipase Cγ (PLCγ)/protein kinase Cα (PKCα)/p38 MAPK pathway. Der f2-induced PLD activation was attenuated by PLCγ inhibitors (U73122 and PAO), PKCα inhibitors (RO320432 and GO6976), and p38 MAPK inhibitors (SB203580 and SB202190). These results indicate that PLCγ, PKCα, and p38 MAPK act as upstream activators of PLD in Der f2-treated BEAS-2B cells. Furthermore, expression and production of IL-13 increased by Der f2 were also blocked by inhibition of PLCγ, PKCα, or p38 MAPK, indicating that IL-13 expression and production are related to a PLCγ/PKCα/p38 MAPK pathway. We found that activating transcription factor-2 (ATF-2) was activated by Der f2 in BEAS-2B cells and activation of ATF-2 was controlled by PLD1. When ATF-2 activity was blocked with ATF-2 siRNA, Der f2-induced IL-13 expression and production were decreased. Thus, ATF-2 might be one of the transcriptional factors for the expression of IL-13 in Der f2-treated BEAS-2B cells. Taken together, PLD1 acts as an important regulator in Der f2-induced expression and production of IL-13 through activation of ATF-2 in BEAS-2B cells.

AB - The purpose of this study was to identify the role of phospholipase D1 (PLD1) inDer f2-induced interleukin (IL)-13 production. The major house dust mite allergen, Der f2, increased PLD activity in human bronchial epithelial cells (BEAS-2B), and dominant negative PLD1 or PLD1 siRNA decreased Der f2-induced IL-13 expression and production. Treatment of Der f2 activated the phospholipase Cγ (PLCγ)/protein kinase Cα (PKCα)/p38 MAPK pathway. Der f2-induced PLD activation was attenuated by PLCγ inhibitors (U73122 and PAO), PKCα inhibitors (RO320432 and GO6976), and p38 MAPK inhibitors (SB203580 and SB202190). These results indicate that PLCγ, PKCα, and p38 MAPK act as upstream activators of PLD in Der f2-treated BEAS-2B cells. Furthermore, expression and production of IL-13 increased by Der f2 were also blocked by inhibition of PLCγ, PKCα, or p38 MAPK, indicating that IL-13 expression and production are related to a PLCγ/PKCα/p38 MAPK pathway. We found that activating transcription factor-2 (ATF-2) was activated by Der f2 in BEAS-2B cells and activation of ATF-2 was controlled by PLD1. When ATF-2 activity was blocked with ATF-2 siRNA, Der f2-induced IL-13 expression and production were decreased. Thus, ATF-2 might be one of the transcriptional factors for the expression of IL-13 in Der f2-treated BEAS-2B cells. Taken together, PLD1 acts as an important regulator in Der f2-induced expression and production of IL-13 through activation of ATF-2 in BEAS-2B cells.

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