Hrp3, a paralog of Hrp1, is a novel member of the CHD1 (chromo-helicase/ATPase-DNA binding 1) protein family of Schizosaccharomyces pombe. Although it has been considered that CHD1 proteins are required for chromatin modifications in transcriptional regulations, little is known about their roles in vivo. In this study, we examined the effects of Hrp3 on heterochromatin silencing using several S. pombe reporter strains. The phenotypic analysis revealed that hrp3+ is not an essential gene for cell viability. However, Hrp3 is required for transcriptional repression at silence loci of mat3. A chromatin immunoprecipitation assay showed that Hrp3 directly associates with mat3 chromatin. Thus, our results strongly suggest that Hrp3 is involved in heterochromatin silencing and plays a direct role as a chromatin remodeling factor at mat3 in vivo.
|Number of pages||5|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 2002|
Bibliographical noteFunding Information:
We thank R. Allshire and G. Thon for providing fission yeast strains, FY1497, FY121, and PG9 and J.A. Huberman for S. pombe rDNA. We also thank Dr. O. Hwang for the critical reading. This work was supported in part by a BK21 Research Fellowship from the Korea Ministry of Education and Korea Research Foundation Grant (R03-2001-00056). Work in the K.E. laboratory was supported by a grant from Cancerfonden #4284-B99.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology