Hrp3, a chromodomain helicase/ATPase DNA binding protein, is required for heterochromatin silencing in fission yeast

Eung Jae Yoo, Yeun Kyu Jang, Myung Ae Lee, Pernilla Bjerling, Jae Bum Kim, Karl Ekwall, Rho Hyun Seong, Sang Dai Park

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Hrp3, a paralog of Hrp1, is a novel member of the CHD1 (chromo-helicase/ATPase-DNA binding 1) protein family of Schizosaccharomyces pombe. Although it has been considered that CHD1 proteins are required for chromatin modifications in transcriptional regulations, little is known about their roles in vivo. In this study, we examined the effects of Hrp3 on heterochromatin silencing using several S. pombe reporter strains. The phenotypic analysis revealed that hrp3+ is not an essential gene for cell viability. However, Hrp3 is required for transcriptional repression at silence loci of mat3. A chromatin immunoprecipitation assay showed that Hrp3 directly associates with mat3 chromatin. Thus, our results strongly suggest that Hrp3 is involved in heterochromatin silencing and plays a direct role as a chromatin remodeling factor at mat3 in vivo.

Original languageEnglish
Pages (from-to)970-974
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume295
Issue number4
DOIs
Publication statusPublished - 2002 Sep 28

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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