Hrp3, a chromodomain helicase/ATPase DNA binding protein, is required for heterochromatin silencing in fission yeast

Eung Jae Yoo, Yeun Kyu Jang, Myung Ae Lee, Pernilla Bjerling, Jae Bum Kim, Karl Ekwall, Rho Hyun Seong, Sang Dai Park

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Hrp3, a paralog of Hrp1, is a novel member of the CHD1 (chromo-helicase/ATPase-DNA binding 1) protein family of Schizosaccharomyces pombe. Although it has been considered that CHD1 proteins are required for chromatin modifications in transcriptional regulations, little is known about their roles in vivo. In this study, we examined the effects of Hrp3 on heterochromatin silencing using several S. pombe reporter strains. The phenotypic analysis revealed that hrp3+ is not an essential gene for cell viability. However, Hrp3 is required for transcriptional repression at silence loci of mat3. A chromatin immunoprecipitation assay showed that Hrp3 directly associates with mat3 chromatin. Thus, our results strongly suggest that Hrp3 is involved in heterochromatin silencing and plays a direct role as a chromatin remodeling factor at mat3 in vivo.

Original languageEnglish
Pages (from-to)970-974
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume295
Issue number4
DOIs
Publication statusPublished - 2002 Sep 28

Fingerprint

Heterochromatin
Schizosaccharomyces
DNA-Binding Proteins
Yeast
Chromatin
Adenosine Triphosphatases
Chromatin Assembly and Disassembly
Chromatin Immunoprecipitation
Essential Genes
Cell Survival
DNA
Assays
Proteins
Genes
Cells

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Jae Yoo, Eung ; Kyu Jang, Yeun ; Ae Lee, Myung ; Bjerling, Pernilla ; Bum Kim, Jae ; Ekwall, Karl ; Hyun Seong, Rho ; Dai Park, Sang. / Hrp3, a chromodomain helicase/ATPase DNA binding protein, is required for heterochromatin silencing in fission yeast. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 295, No. 4. pp. 970-974.
@article{3cf2df31c38043939039d108ff99675a,
title = "Hrp3, a chromodomain helicase/ATPase DNA binding protein, is required for heterochromatin silencing in fission yeast",
abstract = "Hrp3, a paralog of Hrp1, is a novel member of the CHD1 (chromo-helicase/ATPase-DNA binding 1) protein family of Schizosaccharomyces pombe. Although it has been considered that CHD1 proteins are required for chromatin modifications in transcriptional regulations, little is known about their roles in vivo. In this study, we examined the effects of Hrp3 on heterochromatin silencing using several S. pombe reporter strains. The phenotypic analysis revealed that hrp3+ is not an essential gene for cell viability. However, Hrp3 is required for transcriptional repression at silence loci of mat3. A chromatin immunoprecipitation assay showed that Hrp3 directly associates with mat3 chromatin. Thus, our results strongly suggest that Hrp3 is involved in heterochromatin silencing and plays a direct role as a chromatin remodeling factor at mat3 in vivo.",
author = "{Jae Yoo}, Eung and {Kyu Jang}, Yeun and {Ae Lee}, Myung and Pernilla Bjerling and {Bum Kim}, Jae and Karl Ekwall and {Hyun Seong}, Rho and {Dai Park}, Sang",
year = "2002",
month = "9",
day = "28",
doi = "10.1016/S0006-291X(02)00797-0",
language = "English",
volume = "295",
pages = "970--974",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

Hrp3, a chromodomain helicase/ATPase DNA binding protein, is required for heterochromatin silencing in fission yeast. / Jae Yoo, Eung; Kyu Jang, Yeun; Ae Lee, Myung; Bjerling, Pernilla; Bum Kim, Jae; Ekwall, Karl; Hyun Seong, Rho; Dai Park, Sang.

In: Biochemical and Biophysical Research Communications, Vol. 295, No. 4, 28.09.2002, p. 970-974.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Hrp3, a chromodomain helicase/ATPase DNA binding protein, is required for heterochromatin silencing in fission yeast

AU - Jae Yoo, Eung

AU - Kyu Jang, Yeun

AU - Ae Lee, Myung

AU - Bjerling, Pernilla

AU - Bum Kim, Jae

AU - Ekwall, Karl

AU - Hyun Seong, Rho

AU - Dai Park, Sang

PY - 2002/9/28

Y1 - 2002/9/28

N2 - Hrp3, a paralog of Hrp1, is a novel member of the CHD1 (chromo-helicase/ATPase-DNA binding 1) protein family of Schizosaccharomyces pombe. Although it has been considered that CHD1 proteins are required for chromatin modifications in transcriptional regulations, little is known about their roles in vivo. In this study, we examined the effects of Hrp3 on heterochromatin silencing using several S. pombe reporter strains. The phenotypic analysis revealed that hrp3+ is not an essential gene for cell viability. However, Hrp3 is required for transcriptional repression at silence loci of mat3. A chromatin immunoprecipitation assay showed that Hrp3 directly associates with mat3 chromatin. Thus, our results strongly suggest that Hrp3 is involved in heterochromatin silencing and plays a direct role as a chromatin remodeling factor at mat3 in vivo.

AB - Hrp3, a paralog of Hrp1, is a novel member of the CHD1 (chromo-helicase/ATPase-DNA binding 1) protein family of Schizosaccharomyces pombe. Although it has been considered that CHD1 proteins are required for chromatin modifications in transcriptional regulations, little is known about their roles in vivo. In this study, we examined the effects of Hrp3 on heterochromatin silencing using several S. pombe reporter strains. The phenotypic analysis revealed that hrp3+ is not an essential gene for cell viability. However, Hrp3 is required for transcriptional repression at silence loci of mat3. A chromatin immunoprecipitation assay showed that Hrp3 directly associates with mat3 chromatin. Thus, our results strongly suggest that Hrp3 is involved in heterochromatin silencing and plays a direct role as a chromatin remodeling factor at mat3 in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0036375831&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036375831&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(02)00797-0

DO - 10.1016/S0006-291X(02)00797-0

M3 - Article

C2 - 12127990

AN - SCOPUS:0036375831

VL - 295

SP - 970

EP - 974

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -