Human anti-α-enolase antibody in sera from patients with Behçet's disease and rheumatologic disorders

Ju Hee Lee, Sung Bin Cho, Dongsik Bang, Sang Ho Oh, Keun Jae Ahn, Jihyun Kim, Yong Beum Park, Soo Kon Lee, Kwang Hoon Lee

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30 Citations (Scopus)

Abstract

Objective. α-Enolase is a target antigen of IgM-type anti-endothelial cell antibody in patients with Behçet's disease (BD). The objective of this study was to assess the reactivity of serum anti-a-enolase antibodies in BD and in other rheumatologic diseases, and to evaluate the clinical significance of serum anti-a-enolase antibodies in BD. Methods. Enzyme-linked immunosorbent assay (ELISA) and immunoblotting were used to examine serum samples from patients with BD (n=100), systemic lupus erythematosus (SLE) (n=50), systemic sclerosis (n=21), rheumatoid arthritis (RA) (n=20), Takayasu's arteritis (n=20), dermatomyositis (n=17), mixed connective tissue disease (MCTD) (n=11), and samples from healthy volunteer donors (n=23). The medical records of patients with BD were reviewed to investigate their clinical characteristics. Results. Specific positive signals against recombinant human α-enolase were detected by IgM ELISA of serum samples from 56 of the 100 BD patients (56.0%), 24 of the 50 SLE patients (48.0%), 15 of the 21 systemic sclerosis patients (71.4%), 13 of the 20 RA patients (65.0%), 10 of the 20 Takayasu's arteritis patients (50.0%), 9 of the 17 dermatomyositis patients (52.9%), and 5 of the 11 MCTD patients (45.5%). The number of BD patients with vascular lesions was significantly higher in the anti-α-enolase antibody positive group than in the negative group (p=0.027). Conclusions. We demonstrated the reactivities of serum anti-α-enolase antibodies in BD and other rheumatologic diseases with moderate specificity and also found that serum anti-α-enolase antibodies in BD can be associated with vascular system involvement.

Original languageEnglish
Pages (from-to)S63-S66
JournalClinical and experimental rheumatology
Volume27
Issue number2 SUPPL.53
Publication statusPublished - 2009 Oct 26

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All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Immunology and Allergy
  • Immunology

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