Human bone marrow stem cells cultured under hypoxic conditions present altered characteristics and enhanced in vivo tissue regeneration

Jung Seok Lee, Jung Chul Park, Tae Wan Kim, Byung Joo Jung, Youngseok Lee, Eun Kyung Shim, Soyon Park, Eun Young Choi, Kyoo Sung Cho, ChangSung Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Human bone marrow mesenchymal stem cells (hBMSCs) were isolated from bone marrow of the vertebral body. The hBMSCs were cultured under either hypoxic (1% O2) or normoxic (21% O2; control) conditions and the characteristics as mesenchymal stem cells were compared. Results revealed that hypoxia reduced proliferative potential and colony-forming efficiency of hBMSCs, and significantly enhanced osteogenic and chondrogenic differentiation. The hBMSCs enhanced the regenerative potential of bone in vivo. In vitro synthesis of soluble and insoluble collagen was significantly increased in the hypoxic condition. In vivo collagen tissue regeneration was also enhanced under the hypoxic condition, with concomitant increased expressions of various subtypes of collagen and lysyl-oxidase family mRNA. MicroRNA assays revealed that miR-155-5p, which negatively regulates HIF-1α, was significantly highly expressed. These observations demonstrate that hBMSCs obtained from human vertebrae exhibit altered characteristics under hypoxic conditions, and each factor contributing to hBMSC-mediated tissue healing should be evaluated with the goal of allowing their clinical application.

Original languageEnglish
Pages (from-to)34-45
Number of pages12
JournalBone
Volume78
DOIs
Publication statusPublished - 2015 Sep 1

Fingerprint

Mesenchymal Stromal Cells
Bone Marrow Cells
Regeneration
Stem Cells
Bone Marrow
Collagen
Protein-Lysine 6-Oxidase
MicroRNAs
Spine
Bone and Bones
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

Cite this

Lee, Jung Seok ; Park, Jung Chul ; Kim, Tae Wan ; Jung, Byung Joo ; Lee, Youngseok ; Shim, Eun Kyung ; Park, Soyon ; Choi, Eun Young ; Cho, Kyoo Sung ; Kim, ChangSung. / Human bone marrow stem cells cultured under hypoxic conditions present altered characteristics and enhanced in vivo tissue regeneration. In: Bone. 2015 ; Vol. 78. pp. 34-45.
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Human bone marrow stem cells cultured under hypoxic conditions present altered characteristics and enhanced in vivo tissue regeneration. / Lee, Jung Seok; Park, Jung Chul; Kim, Tae Wan; Jung, Byung Joo; Lee, Youngseok; Shim, Eun Kyung; Park, Soyon; Choi, Eun Young; Cho, Kyoo Sung; Kim, ChangSung.

In: Bone, Vol. 78, 01.09.2015, p. 34-45.

Research output: Contribution to journalArticle

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AU - Shim, Eun Kyung

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