Human-induced pluripotent stem cells generated from intervertebral disc cells improve neurologic functions in spinal cord injury

Jinsoo Oh, Kang In Lee, Hyeong Taek Kim, Youngsang You, Do Heum Yoon, Ki Yeong Song, Eunji Cheong, Yoon Ha, Dong Youn Hwang

Research output: Contribution to journalArticle

Abstract

INTRODUCTION: Induced pluripotent stem cells (iPSCs) have emerged as a promising cell source for immune-compatible cell therapy. Although a variety of somatic cells have been tried for iPSC generation, it is still of great interest to test new cell types, especially those which are hardly obtainable in a normal situation.

METHODS: In this study, we generated iPSCs by using the cells originated from intervertebral disc which were removed during a spinal operation after spinal cord injury. We investigated the pluripotency of disc cell-derived iPSCs (diPSCs) and neural differentiation capability as well as therapeutic effect in spinal cord injury.

RESULTS: The diPSCs displayed similar characteristics to human embryonic stem cells and were efficiently differentiated into neural precursor cells (NPCs) with the capability of differentiation into mature neurons in vitro. When the diPSC-derived NPCs were transplanted into mice 9 days after spinal cord injury, we detected a significant amelioration of hindlimb dysfunction during follow-up recovery periods. Histological analysis at 5 weeks after transplantation identified undifferentiated human NPCs (Nestin(+)) as well as early (Tuj1(+)) and mature (MAP2(+)) neurons derived from the transplanted NPCs. Furthermore, NPC transplantation demonstrated a preventive effect on spinal cord degeneration resulting from the secondary injury.

CONCLUSION: This study revealed that intervertebral discs removed during surgery for spinal stabilization after spinal cord injury, previously considered a "waste" tissue, may provide a unique opportunity to study iPSCs derived from difficult-to-access somatic cells and a useful therapeutic resource for autologous cell replacement therapy in spinal cord injury.

Original languageEnglish
Number of pages1
JournalStem cell research & therapy
Volume6
DOIs
Publication statusPublished - 2015 Jun 24

Fingerprint

Induced Pluripotent Stem Cells
Intervertebral Disc
Stem cells
Spinal Cord Injuries
Nervous System
Neurons
Nestin
Cell- and Tissue-Based Therapy
Surgery
Stabilization
Tissue
Cell Transplantation
Recovery
Therapeutic Uses
Hindlimb
Cell Differentiation
Spinal Cord
Transplantation

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Cell Biology

Cite this

Oh, Jinsoo ; Lee, Kang In ; Kim, Hyeong Taek ; You, Youngsang ; Yoon, Do Heum ; Song, Ki Yeong ; Cheong, Eunji ; Ha, Yoon ; Hwang, Dong Youn. / Human-induced pluripotent stem cells generated from intervertebral disc cells improve neurologic functions in spinal cord injury. In: Stem cell research & therapy. 2015 ; Vol. 6.
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Human-induced pluripotent stem cells generated from intervertebral disc cells improve neurologic functions in spinal cord injury. / Oh, Jinsoo; Lee, Kang In; Kim, Hyeong Taek; You, Youngsang; Yoon, Do Heum; Song, Ki Yeong; Cheong, Eunji; Ha, Yoon; Hwang, Dong Youn.

In: Stem cell research & therapy, Vol. 6, 24.06.2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Human-induced pluripotent stem cells generated from intervertebral disc cells improve neurologic functions in spinal cord injury

AU - Oh, Jinsoo

AU - Lee, Kang In

AU - Kim, Hyeong Taek

AU - You, Youngsang

AU - Yoon, Do Heum

AU - Song, Ki Yeong

AU - Cheong, Eunji

AU - Ha, Yoon

AU - Hwang, Dong Youn

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N2 - INTRODUCTION: Induced pluripotent stem cells (iPSCs) have emerged as a promising cell source for immune-compatible cell therapy. Although a variety of somatic cells have been tried for iPSC generation, it is still of great interest to test new cell types, especially those which are hardly obtainable in a normal situation.METHODS: In this study, we generated iPSCs by using the cells originated from intervertebral disc which were removed during a spinal operation after spinal cord injury. We investigated the pluripotency of disc cell-derived iPSCs (diPSCs) and neural differentiation capability as well as therapeutic effect in spinal cord injury.RESULTS: The diPSCs displayed similar characteristics to human embryonic stem cells and were efficiently differentiated into neural precursor cells (NPCs) with the capability of differentiation into mature neurons in vitro. When the diPSC-derived NPCs were transplanted into mice 9 days after spinal cord injury, we detected a significant amelioration of hindlimb dysfunction during follow-up recovery periods. Histological analysis at 5 weeks after transplantation identified undifferentiated human NPCs (Nestin(+)) as well as early (Tuj1(+)) and mature (MAP2(+)) neurons derived from the transplanted NPCs. Furthermore, NPC transplantation demonstrated a preventive effect on spinal cord degeneration resulting from the secondary injury.CONCLUSION: This study revealed that intervertebral discs removed during surgery for spinal stabilization after spinal cord injury, previously considered a "waste" tissue, may provide a unique opportunity to study iPSCs derived from difficult-to-access somatic cells and a useful therapeutic resource for autologous cell replacement therapy in spinal cord injury.

AB - INTRODUCTION: Induced pluripotent stem cells (iPSCs) have emerged as a promising cell source for immune-compatible cell therapy. Although a variety of somatic cells have been tried for iPSC generation, it is still of great interest to test new cell types, especially those which are hardly obtainable in a normal situation.METHODS: In this study, we generated iPSCs by using the cells originated from intervertebral disc which were removed during a spinal operation after spinal cord injury. We investigated the pluripotency of disc cell-derived iPSCs (diPSCs) and neural differentiation capability as well as therapeutic effect in spinal cord injury.RESULTS: The diPSCs displayed similar characteristics to human embryonic stem cells and were efficiently differentiated into neural precursor cells (NPCs) with the capability of differentiation into mature neurons in vitro. When the diPSC-derived NPCs were transplanted into mice 9 days after spinal cord injury, we detected a significant amelioration of hindlimb dysfunction during follow-up recovery periods. Histological analysis at 5 weeks after transplantation identified undifferentiated human NPCs (Nestin(+)) as well as early (Tuj1(+)) and mature (MAP2(+)) neurons derived from the transplanted NPCs. Furthermore, NPC transplantation demonstrated a preventive effect on spinal cord degeneration resulting from the secondary injury.CONCLUSION: This study revealed that intervertebral discs removed during surgery for spinal stabilization after spinal cord injury, previously considered a "waste" tissue, may provide a unique opportunity to study iPSCs derived from difficult-to-access somatic cells and a useful therapeutic resource for autologous cell replacement therapy in spinal cord injury.

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