Human leukocyte antigen-G (HLA-G) polymorphism and expression in breast cancer patients

Seri Jeong, Seho Park, Byeong Woo Park, Younhee Park, Oh Joong Kwon, Hyon Suk Kim

Research output: Contribution to journalArticle

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Abstract

Human leukocyte antigen-G (HLA-G) is known to be implicated in a tumor-driven immune escape mechanism in malignancies. The purpose of this study was to investigate HLA-G polymorphism and expression in breast cancer. HLA-G alleles were determined by direct DNA sequencing procedures from blood samples of 80 breast cancer patients and 80 healthy controls. Soluble HLA-G (sHLA-G) was measured by enzyme-linked immunosorbent assay (ELISA) from serum specimens. HLA-G expression in breast cancer lesions was also analyzed by immunohistochemistry staining. The presence of HLA-G 3′ untranslated region (UTR) 14-bp sequence was analyzed and found to be associated with reduced risk of breast cancer susceptibility based on HLA-G expression in tissues (P = 0.0407). Levels of sHLA-G were higher in the breast cancer group (median 117.2 U/mL) compared to the control group (median 10.1 U/mL, P<0.001). The area under the receiver operating characteristic curve (AU-ROC) values of sHLA-G for differentiating breast cancer from normal controls and for detecting metastasis from other stages of breast cancer were 0.89 and 0.79, respectively. HLA-G polymorphism and expression may be involved in breast carcinogenesis and sHLA-G concentrations could be used as a diagnostic marker for detecting breast cancer.

Original languageEnglish
Article numbere98284
JournalPloS one
Volume9
Issue number5
DOIs
Publication statusPublished - 2014 May 28

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HLA Antigens
Polymorphism
breast neoplasms
genetic polymorphism
Breast Neoplasms
HLA-G Antigens
Tumor Escape
Immunosorbents
HLA antigens
3' Untranslated Regions
3' untranslated regions
blood serum
DNA Sequence Analysis
ROC Curve
lesions (animal)
metastasis
carcinogenesis
breasts
immunohistochemistry
Tumors

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Jeong, Seri ; Park, Seho ; Park, Byeong Woo ; Park, Younhee ; Kwon, Oh Joong ; Kim, Hyon Suk. / Human leukocyte antigen-G (HLA-G) polymorphism and expression in breast cancer patients. In: PloS one. 2014 ; Vol. 9, No. 5.
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Human leukocyte antigen-G (HLA-G) polymorphism and expression in breast cancer patients. / Jeong, Seri; Park, Seho; Park, Byeong Woo; Park, Younhee; Kwon, Oh Joong; Kim, Hyon Suk.

In: PloS one, Vol. 9, No. 5, e98284, 28.05.2014.

Research output: Contribution to journalArticle

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