Human Polycomb protein 2 promotes α-synuclein aggregate formation through covalent SUMOylation

Yohan Oh, Yong Man Kim, M. Maral Mouradian, Kwang Chul Chung

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Parkinson's disease (PD) manifests from the impairment of motor systems due to the specific loss of dopaminergic neurons and the appearance of intracellular filamentous inclusions called Lewy bodies (LBs). α-Synuclein, a major component of LBs, is known to contribute to the pathogenesis of PD. Although α-synuclein is known to be a target of diverse posttranslational modifications, the contribution of α-synuclein SUMOylation and its functional consequences have not yet been fully characterized. Here, we demonstrate that human Polycomb protein 2 (hPc2) binds to α-synuclein and may function as a SUMO E3 ligase to promote the SUMOylation of α-synuclein. In addition, hPc2 promotes the SUMOylation of α-synuclein in the presence of MG-132-induced proteasome inhibition, which consequently promotes α-synuclein aggregate formation. Furthermore, the increased formation of intracellular α-synuclein aggregates, which predominantly contain SUMOylated α-synuclein, significantly reduces the death of fibroblast cells in response to staurosporine. In summary, the results from this study demonstrate that the hPc2-induced SUMOylation of α-synuclein could function as a cytoprotector by increasing α-synuclein aggregate formation within fibroblast cells.

Original languageEnglish
Pages (from-to)78-89
Number of pages12
JournalBrain Research
Volume1381
DOIs
Publication statusPublished - 2011 Mar 24

Fingerprint

Synucleins
Sumoylation
Proteins
Lewy Bodies
Parkinson Disease
Fibroblasts
Ubiquitin-Protein Ligases
Staurosporine
Dopaminergic Neurons
Proteasome Endopeptidase Complex
Post Translational Protein Processing

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Oh, Yohan ; Kim, Yong Man ; Mouradian, M. Maral ; Chung, Kwang Chul. / Human Polycomb protein 2 promotes α-synuclein aggregate formation through covalent SUMOylation. In: Brain Research. 2011 ; Vol. 1381. pp. 78-89.
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Human Polycomb protein 2 promotes α-synuclein aggregate formation through covalent SUMOylation. / Oh, Yohan; Kim, Yong Man; Mouradian, M. Maral; Chung, Kwang Chul.

In: Brain Research, Vol. 1381, 24.03.2011, p. 78-89.

Research output: Contribution to journalArticle

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