Human Telomerase Reverse Transcriptase (hTERT) Positively Regulates 26S Proteasome Activity

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Human telomerase reverse transcriptase (hTERT) is the catalytic subunit of telomerase, an RNA-dependent DNA polymerase that elongates telomeric DNA. hTERT displays several extra-telomeric functions that are independent of its telomere-regulatory function, including tumor progression, and neuronal cell death regulation. In this study, we evaluated these additional hTERT non-telomeric functions. We determined that hTERT interacts with several 19S and 20S proteasome subunits. The 19S regulatory particle and 20S core particle are part of 26S proteasome complex, which plays a central role in ubiquitin-dependent proteolysis. In addition, hTERT positively regulated 26S proteasome activity independent of its enzymatic activity. Moreover, hTERT enhanced subunit interactions, which may underlie hTERT's ability of hTERT to stimulate the 26S proteasome. Furthermore, hTERT displayed cytoprotective effect against ER stress via the activation of 26S proteasome in acute myeloid leukemia cells. Our data suggest that hTERT acts as a novel chaperone to promote 26S proteasome assembly and maintenance. J. Cell. Physiol. 232: 2083–2093, 2017.

Original languageEnglish
Pages (from-to)2083-2093
Number of pages11
JournalJournal of Cellular Physiology
Volume232
Issue number8
DOIs
Publication statusPublished - 2017 Aug

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Human Telomerase Reverse Transcriptase (hTERT) Positively Regulates 26S Proteasome Activity'. Together they form a unique fingerprint.

  • Cite this