Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas

Young Jin Park, Eun Kyoung Kim, Sook Moon, Doo Pyo Hong, Jung Yoon Bae, Jin Kim

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Background/Aim: The present study aimed to investigate whether the down-regulation of human telomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. Materials and Methods: A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed. Results: The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line. Conclusion: Targeting hTERT may lead to novel therapeutic approaches.

Original languageEnglish
Pages (from-to)6389-6395
Number of pages7
JournalAnticancer research
Volume34
Issue number11
Publication statusPublished - 2014 Nov 1

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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