Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas

Young Jin Park; Eun Kyoung Kim; Sook Moon; Doo Pyo Hong; Jung Yoon Bae; Jin Kim

Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas

Young Jin Park, Eun Kyoung Kim, Sook Moon, Doo Pyo Hong, Jung Yoon Bae, Jin Kim

Department of Oral Pathology

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8 Citations (Scopus)

Abstract

Background/Aim: The present study aimed to investigate whether the down-regulation of human telomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. Materials and Methods: A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed. Results: The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line. Conclusion: Targeting hTERT may lead to novel therapeutic approaches.

Original language	English
Pages (from-to)	6389-6395
Number of pages	7
Journal	Anticancer Research
Volume	34
Issue number	11
Publication status	Published - 2014 Jan 1

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All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

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Park, Y. J., Kim, E. K., Moon, S., Hong, D. P., Bae, J. Y., & Kim, J. (2014). Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas. Anticancer Research, 34(11), 6389-6395.

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abstract = "Background/Aim: The present study aimed to investigate whether the down-regulation of human telomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. Materials and Methods: A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed. Results: The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line. Conclusion: Targeting hTERT may lead to novel therapeutic approaches.",

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T1 - Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas

AU - Park, Young Jin

AU - Kim, Eun Kyoung

AU - Moon, Sook

AU - Hong, Doo Pyo

AU - Bae, Jung Yoon

AU - Kim, Jin

PY - 2014/1/1

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N2 - Background/Aim: The present study aimed to investigate whether the down-regulation of human telomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. Materials and Methods: A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed. Results: The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line. Conclusion: Targeting hTERT may lead to novel therapeutic approaches.

AB - Background/Aim: The present study aimed to investigate whether the down-regulation of human telomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. Materials and Methods: A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed. Results: The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line. Conclusion: Targeting hTERT may lead to novel therapeutic approaches.

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Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas

Abstract

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All Science Journal Classification (ASJC) codes

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