Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas

Young Jin Park; Eun Kyoung Kim; Sook Moon; Doo Pyo Hong; Jung Yoon Bae; Jin Kim

Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas

Young Jin Park, Eun Kyoung Kim, Sook Moon, Doo Pyo Hong, Jung Yoon Bae, Jin Kim

Department of Oral Pathology

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Background/Aim: The present study aimed to investigate whether the down-regulation of human telomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. Materials and Methods: A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed. Results: The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line. Conclusion: Targeting hTERT may lead to novel therapeutic approaches.

Original language	English
Pages (from-to)	6389-6395
Number of pages	7
Journal	Anticancer research
Volume	34
Issue number	11
Publication status	Published - 2014 Nov 1

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

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title = "Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas",

abstract = "Background/Aim: The present study aimed to investigate whether the down-regulation of human telomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. Materials and Methods: A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed. Results: The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line. Conclusion: Targeting hTERT may lead to novel therapeutic approaches.",

author = "Park, {Young Jin} and Kim, {Eun Kyoung} and Sook Moon and Hong, {Doo Pyo} and Bae, {Jung Yoon} and Jin Kim",

year = "2014",

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TY - JOUR

T1 - Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas

AU - Park, Young Jin

AU - Kim, Eun Kyoung

AU - Moon, Sook

AU - Hong, Doo Pyo

AU - Bae, Jung Yoon

AU - Kim, Jin

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Background/Aim: The present study aimed to investigate whether the down-regulation of human telomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. Materials and Methods: A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed. Results: The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line. Conclusion: Targeting hTERT may lead to novel therapeutic approaches.

AB - Background/Aim: The present study aimed to investigate whether the down-regulation of human telomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. Materials and Methods: A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed. Results: The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line. Conclusion: Targeting hTERT may lead to novel therapeutic approaches.

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