Abstract
Background/Aim: The present study aimed to investigate whether the down-regulation of human telomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. Materials and Methods: A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed. Results: The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line. Conclusion: Targeting hTERT may lead to novel therapeutic approaches.
| Original language | English |
|---|---|
| Pages (from-to) | 6389-6395 |
| Number of pages | 7 |
| Journal | Anticancer Research |
| Volume | 34 |
| Issue number | 11 |
| Publication status | Published - 2014 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research
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Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas. / Park, Young Jin; Kim, Eun Kyoung; Moon, Sook; Hong, Doo Pyo; Bae, Jung Yoon; Kim, Jin.
In: Anticancer Research, Vol. 34, No. 11, 01.01.2014, p. 6389-6395.Research output: Contribution to journal › Article
TY - JOUR
T1 - Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas
AU - Park, Young Jin
AU - Kim, Eun Kyoung
AU - Moon, Sook
AU - Hong, Doo Pyo
AU - Bae, Jung Yoon
AU - Kim, Jin
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Background/Aim: The present study aimed to investigate whether the down-regulation of human telomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. Materials and Methods: A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed. Results: The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line. Conclusion: Targeting hTERT may lead to novel therapeutic approaches.
AB - Background/Aim: The present study aimed to investigate whether the down-regulation of human telomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. Materials and Methods: A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed. Results: The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line. Conclusion: Targeting hTERT may lead to novel therapeutic approaches.
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M3 - Article
VL - 34
SP - 6389
EP - 6395
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 11
ER -