Hydrogen and methane production via a two-stage processes (H2-SBR + CH4-UASB) using tequila vinasses

Germán Buitrón, Gopalakrishnan Kumar, Andrés Martinez-Arce, Gloria Moreno

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78 Citations (Scopus)


The feasibility of producing hydrogen and methane via a two-stage fermentation of tequila vinasses was evaluated in sequencing batch (SBR) and up-flow anaerobic sludge blanket (UASB) reactors. Different vinasses concentrations ranging from 500 mg COD/L to 16 g COD/L were studied in SBR by using thermally pre-treated anaerobic sludge as inoculum for hydrogen production. Peak volumetric hydrogen production rate and specific hydrogen production were attained as 57.4 ± 4.0 mL H2/L-h and 918 ± 63 mL H2/gVSS-d, at the substrate concentration of 16 g COD/L and 6 h of hydraulic retention time (HRT). Increasing substrate concentration has no effect on the specific hydrogen production rate. The fermentation effluent was used for methane production in an UASB reactor. The higher methane composition in the biogas was achieved as 68% at an influent concentration of 1636 mg COD/L. Peak methane volumetric, specific production rates and yield were attained as 11.7 ± 0.7 mL CH4/L-h, 7.2 ± 0.4 mL CH4/g COD-h and 257.9 ± 13.8 mL CH4/g COD at 24 h-HRT and a substrate concentration of 1636 mg COD/L. An overall organic matter removal (SBR + UASB) in this two-stage process of 73-75% was achieved.

Original languageEnglish
Pages (from-to)19249-19255
Number of pages7
JournalInternational Journal of Hydrogen Energy
Issue number33
Publication statusPublished - 2014 Nov 11

Bibliographical note

Funding Information:
The financial support for this project was provided by the Consejo Nacional de Ciencia y Tecnologia (CONACyT) through grant 100298 . Jaime Perez Trevilla is acknowledged for his technical assistance.

Publisher Copyright:
© 2014 Hydrogen Energy Publications, LLC. All rights reserved.

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


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