Hyper biohydrogen production from xylose and xylose-based hemicellulose biomass by the novel strain Clostridium sp. YD09

Sang Hyun Kim; Ye Da Yi; Hyun Joong Kim; Shashi Kant Bhatia; Ranjit Gurav; Jong Min Jeon; Jeong Jun Yoon; Sang Hyoun Kim; Jeong Hoon Park; Yung Hun Yang

doi:10.1016/j.bej.2022.108624

Hyper biohydrogen production from xylose and xylose-based hemicellulose biomass by the novel strain Clostridium sp. YD09

Sang Hyun Kim, Ye Da Yi, Hyun Joong Kim, Shashi Kant Bhatia, Ranjit Gurav, Jong Min Jeon, Jeong Jun Yoon, Sang Hyoun Kim, Jeong Hoon Park, Yung Hun Yang

Department of Civil and Environmental Engineering

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

With increasing interest in biohydrogen as an alternative fuel to petroleum, it is essential to identify a hyper biohydrogen producer. In the present study, Clostridium sp. YD09 was isolated from a brewery wastewater upflow anaerobic sludge blanket digestion reactor; its 16S ribosomal RNA sequencing analysis showed 96 % similarity to Clostridium beijerinckii. Clostridium sp. YD09 produced the highest cumulative volume of hydrogen using xylose as the substrate (optimal concentration of 10 g/L) to obtain 1.21 mol H₂/mol xylose. Furthermore, Clostridium sp. YD09 tolerates various inhibitors from pre-treated lignocellulosic biomass up to a 0.1 % concentration. A maximum yield of 1.62 mol H₂/mol xylose and 1.98 mL H2/mL media with 38- to 48-fold increase was recorded in ligno-hemicellulose xylose treated with biochar and activated carbon. The newly isolated Clostridium sp. YD09 can therefore be used for efficient biohydrogen production from xylose-based substrates.

Original language	English
Article number	108624
Journal	Biochemical Engineering Journal
Volume	187
DOIs	https://doi.org/10.1016/j.bej.2022.108624
Publication status	Published - 2022 Nov

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation of Korea (NRF) ( NRF-2019M3E6A1103979 , NRF-2022R1A2C2003138 , NRF-2022M3I3A1082545 , and NRF-2022M3J4A1053702 ) and the R&D Program of MOTIE/KEIT (grant number 20018072 ).

Publisher Copyright:
© 2022 Elsevier B.V.

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Environmental Engineering
Biomedical Engineering

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10.1016/j.bej.2022.108624

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title = "Hyper biohydrogen production from xylose and xylose-based hemicellulose biomass by the novel strain Clostridium sp. YD09",

abstract = "With increasing interest in biohydrogen as an alternative fuel to petroleum, it is essential to identify a hyper biohydrogen producer. In the present study, Clostridium sp. YD09 was isolated from a brewery wastewater upflow anaerobic sludge blanket digestion reactor; its 16S ribosomal RNA sequencing analysis showed 96 % similarity to Clostridium beijerinckii. Clostridium sp. YD09 produced the highest cumulative volume of hydrogen using xylose as the substrate (optimal concentration of 10 g/L) to obtain 1.21 mol H2/mol xylose. Furthermore, Clostridium sp. YD09 tolerates various inhibitors from pre-treated lignocellulosic biomass up to a 0.1 % concentration. A maximum yield of 1.62 mol H2/mol xylose and 1.98 mL H2/mL media with 38- to 48-fold increase was recorded in ligno-hemicellulose xylose treated with biochar and activated carbon. The newly isolated Clostridium sp. YD09 can therefore be used for efficient biohydrogen production from xylose-based substrates.",

author = "Kim, {Sang Hyun} and {Da Yi}, Ye and Kim, {Hyun Joong} and Bhatia, {Shashi Kant} and Ranjit Gurav and Jeon, {Jong Min} and Yoon, {Jeong Jun} and Kim, {Sang Hyoun} and Park, {Jeong Hoon} and Yang, {Yung Hun}",

note = "Funding Information: This study was supported by the National Research Foundation of Korea (NRF) ( NRF-2019M3E6A1103979 , NRF-2022R1A2C2003138 , NRF-2022M3I3A1082545 , and NRF-2022M3J4A1053702 ) and the R&D Program of MOTIE/KEIT (grant number 20018072 ). Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

year = "2022",

month = nov,

doi = "10.1016/j.bej.2022.108624",

language = "English",

volume = "187",

journal = "Biochemical Engineering Journal",

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AU - Kim, Sang Hyun

AU - Da Yi, Ye

AU - Kim, Hyun Joong

AU - Bhatia, Shashi Kant

AU - Gurav, Ranjit

AU - Jeon, Jong Min

AU - Yoon, Jeong Jun

AU - Kim, Sang Hyoun

AU - Park, Jeong Hoon

AU - Yang, Yung Hun

N1 - Funding Information: This study was supported by the National Research Foundation of Korea (NRF) ( NRF-2019M3E6A1103979 , NRF-2022R1A2C2003138 , NRF-2022M3I3A1082545 , and NRF-2022M3J4A1053702 ) and the R&D Program of MOTIE/KEIT (grant number 20018072 ). Publisher Copyright: © 2022 Elsevier B.V.

PY - 2022/11

Y1 - 2022/11

N2 - With increasing interest in biohydrogen as an alternative fuel to petroleum, it is essential to identify a hyper biohydrogen producer. In the present study, Clostridium sp. YD09 was isolated from a brewery wastewater upflow anaerobic sludge blanket digestion reactor; its 16S ribosomal RNA sequencing analysis showed 96 % similarity to Clostridium beijerinckii. Clostridium sp. YD09 produced the highest cumulative volume of hydrogen using xylose as the substrate (optimal concentration of 10 g/L) to obtain 1.21 mol H2/mol xylose. Furthermore, Clostridium sp. YD09 tolerates various inhibitors from pre-treated lignocellulosic biomass up to a 0.1 % concentration. A maximum yield of 1.62 mol H2/mol xylose and 1.98 mL H2/mL media with 38- to 48-fold increase was recorded in ligno-hemicellulose xylose treated with biochar and activated carbon. The newly isolated Clostridium sp. YD09 can therefore be used for efficient biohydrogen production from xylose-based substrates.

AB - With increasing interest in biohydrogen as an alternative fuel to petroleum, it is essential to identify a hyper biohydrogen producer. In the present study, Clostridium sp. YD09 was isolated from a brewery wastewater upflow anaerobic sludge blanket digestion reactor; its 16S ribosomal RNA sequencing analysis showed 96 % similarity to Clostridium beijerinckii. Clostridium sp. YD09 produced the highest cumulative volume of hydrogen using xylose as the substrate (optimal concentration of 10 g/L) to obtain 1.21 mol H2/mol xylose. Furthermore, Clostridium sp. YD09 tolerates various inhibitors from pre-treated lignocellulosic biomass up to a 0.1 % concentration. A maximum yield of 1.62 mol H2/mol xylose and 1.98 mL H2/mL media with 38- to 48-fold increase was recorded in ligno-hemicellulose xylose treated with biochar and activated carbon. The newly isolated Clostridium sp. YD09 can therefore be used for efficient biohydrogen production from xylose-based substrates.

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Hyper biohydrogen production from xylose and xylose-based hemicellulose biomass by the novel strain Clostridium sp. YD09

Abstract

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