Hyper Sensitive Strip Test with Chemi-luminescence Signal Band

Hyung Seok Kim, Jae Chul Pyun

Research output: Contribution to journalConference article

10 Citations (Scopus)

Abstract

A simple and sensitive method using lateral flow immunoassay (LF) using chemiluminescence reaction has been developed for the detection of antigen. In this work, horseradish peroxidase (HRP) samples were used to confirm the validity of this method and human chorionic gonadotropin (hCG) samples were used to evaluate the limit of detection (LOD) of this method. Through HRP test, this method was confirmed to be reliable for immunoassay, and the results of hCG test show that this method was very sensitive compared with conventional strip test using color of gold nanoparticles or latex beads.

Original languageEnglish
Pages (from-to)1043-1046
Number of pages4
JournalProcedia Chemistry
Volume1
Issue number1
DOIs
Publication statusPublished - 2009 Sep 1
EventEurosensors 23rd Conference - Lausanne, Switzerland
Duration: 2009 Sep 62009 Sep 9

Fingerprint

Chorionic Gonadotropin
Horseradish Peroxidase
Luminescence
Chemiluminescence
Latex
Antigens
Latexes
Gold
Nanoparticles
Color
Gonadotropins

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

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Hyper Sensitive Strip Test with Chemi-luminescence Signal Band. / Kim, Hyung Seok; Pyun, Jae Chul.

In: Procedia Chemistry, Vol. 1, No. 1, 01.09.2009, p. 1043-1046.

Research output: Contribution to journalConference article

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AB - A simple and sensitive method using lateral flow immunoassay (LF) using chemiluminescence reaction has been developed for the detection of antigen. In this work, horseradish peroxidase (HRP) samples were used to confirm the validity of this method and human chorionic gonadotropin (hCG) samples were used to evaluate the limit of detection (LOD) of this method. Through HRP test, this method was confirmed to be reliable for immunoassay, and the results of hCG test show that this method was very sensitive compared with conventional strip test using color of gold nanoparticles or latex beads.

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