Hyperlayer hollow-fiber flow field-flow fractionation of cells

Pierluigi Reschiglian, Andrea Zattoni, Barbara Roda, Leonardo Cinque, Dora Melucci, Byung Ryul Min, Myeong Hee Moon

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Interest in low-cost, analytical-scale, highly efficient and sensitive separation methods for cells, among which bacteria, is increasing. Particle separation in hollow-fiber flow field-flow fractionation (HF FlFFF) has been recently improved by the optimization of the HF FlFFF channel design. The intrinsic simplicity and low cost of this HF FlFFF channel allows for its disposable usage, which is particularly appealing for analytical bio-applications. Here, for the first time, we present a feasibility study on high-performance, hyperlayer HF FlFFF of micrometer-sized bacteria (Escherichia coli) and of different types of cells (human red blood cells, wine-making yeast from Saccharomyces cerevisiae). Fractionation performance is shown to be at least comparable to that obtained with conventional, flat-channel hyperlayer FlFFF of cells, at superior size-based selectivity and reduced analysis time.

Original languageEnglish
Pages (from-to)519-529
Number of pages11
JournalJournal of Chromatography A
Volume985
Issue number1-2
DOIs
Publication statusPublished - 2003 Jan 24

Fingerprint

Field Flow Fractionation
Fractionation
Flow fields
Fibers
Yeast
Bacteria
Costs and Cost Analysis
Cell Separation
Feasibility Studies
Wine
Saccharomyces cerevisiae
Yeasts
Erythrocytes
Escherichia coli
Costs
Blood
Cells

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Reschiglian, Pierluigi ; Zattoni, Andrea ; Roda, Barbara ; Cinque, Leonardo ; Melucci, Dora ; Min, Byung Ryul ; Moon, Myeong Hee. / Hyperlayer hollow-fiber flow field-flow fractionation of cells. In: Journal of Chromatography A. 2003 ; Vol. 985, No. 1-2. pp. 519-529.
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Reschiglian, P, Zattoni, A, Roda, B, Cinque, L, Melucci, D, Min, BR & Moon, MH 2003, 'Hyperlayer hollow-fiber flow field-flow fractionation of cells', Journal of Chromatography A, vol. 985, no. 1-2, pp. 519-529. https://doi.org/10.1016/S0021-9673(02)01458-9

Hyperlayer hollow-fiber flow field-flow fractionation of cells. / Reschiglian, Pierluigi; Zattoni, Andrea; Roda, Barbara; Cinque, Leonardo; Melucci, Dora; Min, Byung Ryul; Moon, Myeong Hee.

In: Journal of Chromatography A, Vol. 985, No. 1-2, 24.01.2003, p. 519-529.

Research output: Contribution to journalArticle

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Reschiglian P, Zattoni A, Roda B, Cinque L, Melucci D, Min BR et al. Hyperlayer hollow-fiber flow field-flow fractionation of cells. Journal of Chromatography A. 2003 Jan 24;985(1-2):519-529. https://doi.org/10.1016/S0021-9673(02)01458-9