Interest in low-cost, analytical-scale, highly efficient and sensitive separation methods for cells, among which bacteria, is increasing. Particle separation in hollow-fiber flow field-flow fractionation (HF FlFFF) has been recently improved by the optimization of the HF FlFFF channel design. The intrinsic simplicity and low cost of this HF FlFFF channel allows for its disposable usage, which is particularly appealing for analytical bio-applications. Here, for the first time, we present a feasibility study on high-performance, hyperlayer HF FlFFF of micrometer-sized bacteria (Escherichia coli) and of different types of cells (human red blood cells, wine-making yeast from Saccharomyces cerevisiae). Fractionation performance is shown to be at least comparable to that obtained with conventional, flat-channel hyperlayer FlFFF of cells, at superior size-based selectivity and reduced analysis time.
Bibliographical noteFunding Information:
Hollow fibers were kindly supplied by SKU and Sambo (South Korea). E.coli strains were supplied by SBL Vaccin AB (Solna, Sweden), within Leonardo-EXCHANGE 2001, financial program for researchers mobility from the University of Bologna to small and medium enterprises within the EU. LC’s research stay at the Department of Chemical Engineering, Yonsey University, Seoul (South Korea), was kindly supported by the Faculty of Science of the University of Bologna and by Yonsei University. Fresh blood samples were supplied and clinically tested by the Department of Internal Medicine and Gastroenterology, University of Bologna. E. Whitmore-Carlsson, SBL Vaccin, is duly acknowledged for accurate details on sample specifications and the helpful discussion. Thanks also goes to M. Massari for the experimental work performed, and to A. Roda for the helpful discussion on the properties of bile acids.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Organic Chemistry