Hyperlayer hollow-fiber flow field-flow fractionation of cells

Pierluigi Reschiglian; Andrea Zattoni; Barbara Roda; Leonardo Cinque; Dora Melucci; Byung Ryul Min; Myeong Hee Moon

doi:10.1016/S0021-9673(02)01458-9

Hyperlayer hollow-fiber flow field-flow fractionation of cells

Pierluigi Reschiglian, Andrea Zattoni, Barbara Roda, Leonardo Cinque, Dora Melucci, Byung Ryul Min, Myeong Hee Moon

Research output: Contribution to journal › Article › peer-review

57 Citations (Scopus)

Abstract

Interest in low-cost, analytical-scale, highly efficient and sensitive separation methods for cells, among which bacteria, is increasing. Particle separation in hollow-fiber flow field-flow fractionation (HF FlFFF) has been recently improved by the optimization of the HF FlFFF channel design. The intrinsic simplicity and low cost of this HF FlFFF channel allows for its disposable usage, which is particularly appealing for analytical bio-applications. Here, for the first time, we present a feasibility study on high-performance, hyperlayer HF FlFFF of micrometer-sized bacteria (Escherichia coli) and of different types of cells (human red blood cells, wine-making yeast from Saccharomyces cerevisiae). Fractionation performance is shown to be at least comparable to that obtained with conventional, flat-channel hyperlayer FlFFF of cells, at superior size-based selectivity and reduced analysis time.

Original language	English
Pages (from-to)	519-529
Number of pages	11
Journal	Journal of Chromatography A
Volume	985
Issue number	1-2
DOIs	https://doi.org/10.1016/S0021-9673(02)01458-9
Publication status	Published - 2003 Jan 24

Bibliographical note

Funding Information:
Hollow fibers were kindly supplied by SKU and Sambo (South Korea). E.coli strains were supplied by SBL Vaccin AB (Solna, Sweden), within Leonardo-EXCHANGE 2001, financial program for researchers mobility from the University of Bologna to small and medium enterprises within the EU. LC’s research stay at the Department of Chemical Engineering, Yonsey University, Seoul (South Korea), was kindly supported by the Faculty of Science of the University of Bologna and by Yonsei University. Fresh blood samples were supplied and clinically tested by the Department of Internal Medicine and Gastroenterology, University of Bologna. E. Whitmore-Carlsson, SBL Vaccin, is duly acknowledged for accurate details on sample specifications and the helpful discussion. Thanks also goes to M. Massari for the experimental work performed, and to A. Roda for the helpful discussion on the properties of bile acids.

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/S0021-9673(02)01458-9

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abstract = "Interest in low-cost, analytical-scale, highly efficient and sensitive separation methods for cells, among which bacteria, is increasing. Particle separation in hollow-fiber flow field-flow fractionation (HF FlFFF) has been recently improved by the optimization of the HF FlFFF channel design. The intrinsic simplicity and low cost of this HF FlFFF channel allows for its disposable usage, which is particularly appealing for analytical bio-applications. Here, for the first time, we present a feasibility study on high-performance, hyperlayer HF FlFFF of micrometer-sized bacteria (Escherichia coli) and of different types of cells (human red blood cells, wine-making yeast from Saccharomyces cerevisiae). Fractionation performance is shown to be at least comparable to that obtained with conventional, flat-channel hyperlayer FlFFF of cells, at superior size-based selectivity and reduced analysis time.",

author = "Pierluigi Reschiglian and Andrea Zattoni and Barbara Roda and Leonardo Cinque and Dora Melucci and Min, {Byung Ryul} and Moon, {Myeong Hee}",

note = "Funding Information: Hollow fibers were kindly supplied by SKU and Sambo (South Korea). E.coli strains were supplied by SBL Vaccin AB (Solna, Sweden), within Leonardo-EXCHANGE 2001, financial program for researchers mobility from the University of Bologna to small and medium enterprises within the EU. LC{\textquoteright}s research stay at the Department of Chemical Engineering, Yonsey University, Seoul (South Korea), was kindly supported by the Faculty of Science of the University of Bologna and by Yonsei University. Fresh blood samples were supplied and clinically tested by the Department of Internal Medicine and Gastroenterology, University of Bologna. E. Whitmore-Carlsson, SBL Vaccin, is duly acknowledged for accurate details on sample specifications and the helpful discussion. Thanks also goes to M. Massari for the experimental work performed, and to A. Roda for the helpful discussion on the properties of bile acids.",

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AU - Min, Byung Ryul

AU - Moon, Myeong Hee

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Hyperlayer hollow-fiber flow field-flow fractionation of cells

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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