Abstract
Many kinds of susceptibility test for β-lactam antibiotics have been used to determine the antibiotic resistance of bacterial strains. Here, a sensitive antibiotic susceptibility test was presented by using a specialized reaction tool for laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS) based on parylene-matrix chip. The β-lactamase assay was carried out in a specialized reaction tool by (1) concentrating the bacterial strain and (2) incubating the bacteria with penicillin-G. The parylene-matrix chip was produced by deposition of a partially porous parylene-N thin film on a dried organic matrix array, and the products of β-lactamase reaction in the low range of mass-to-charge ratio (m/z < 500) could be effectively analyzed by using a parylene-matrix chip. The sensing parameters were compared with conventional chromogenic antibiotic susceptibility test for β-lactam antibiotics. Finally, LDI-TOF MS with a specialized reaction tool and parylene-matrix chip could achieve a limit of detection as low as 600 cells/spot for penicillin-G.
Original language | English |
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Pages (from-to) | 90-96 |
Number of pages | 7 |
Journal | Enzyme and Microbial Technology |
Volume | 97 |
DOIs | |
Publication status | Published - 2017 Feb 1 |
Bibliographical note
Funding Information:This research was supported by the National Research Foundation of Korea ( 2014M3A9E5073818 ) and by the Korean Small and Medium Business Administration ( S2220656 ).
Publisher Copyright:
© 2016 Elsevier Inc.
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology