Hypersensitive immunoassay by using Escherichia coli outer membrane with autodisplayed Z-domains

Min Park, Joachim Jose, Jae Chul Pyun

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

In this work, the Z-domain of protein A was autodisplayed on the surface of the Escherichia coli outer membrane as a fusion protein of AIDA-1, and then layered on a microplate in order to develop a hypersensitive immunoassay. By using microplates with controlled hydrophilicity the formation of outer membrane layer was carried out, and the driving force for the layer formation was proven to be a hydrophobic interactions. Through the orientation control of the immobilized antibodies by using autodisplayed Z-domains, the limit of detection (LOD) and the sensitivity of this new immunoassay were determined to have improved as much as 30-fold compared to the conventional ELISA. The applicability of the new immunoassay for medical diagnosis was demonstrated by the detection of C-reactive protein (CRP) which is known to be a biomarker protein for inflammatory diseases.

Original languageEnglish
Pages (from-to)309-314
Number of pages6
JournalEnzyme and Microbial Technology
Volume46
Issue number3-4
DOIs
Publication statusPublished - 2010 Mar 5

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0062890, R15-2004-024-00000-0 and 2009-008-1529) and by “System IC 2010” project of Ministry of Knowledge Economy and by the Seoul Research and Business Development Program (10816).

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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