Abstract
In this work, the Z-domain of protein A was autodisplayed on the surface of the Escherichia coli outer membrane as a fusion protein of AIDA-1, and then layered on a microplate in order to develop a hypersensitive immunoassay. By using microplates with controlled hydrophilicity the formation of outer membrane layer was carried out, and the driving force for the layer formation was proven to be a hydrophobic interactions. Through the orientation control of the immobilized antibodies by using autodisplayed Z-domains, the limit of detection (LOD) and the sensitivity of this new immunoassay were determined to have improved as much as 30-fold compared to the conventional ELISA. The applicability of the new immunoassay for medical diagnosis was demonstrated by the detection of C-reactive protein (CRP) which is known to be a biomarker protein for inflammatory diseases.
| Original language | English |
|---|---|
| Pages (from-to) | 309-314 |
| Number of pages | 6 |
| Journal | Enzyme and Microbial Technology |
| Volume | 46 |
| Issue number | 3-4 |
| DOIs | |
| Publication status | Published - 2010 Mar 5 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology
Cite this
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Hypersensitive immunoassay by using Escherichia coli outer membrane with autodisplayed Z-domains. / Park, Min; Jose, Joachim; Pyun, Jae Chul.
In: Enzyme and Microbial Technology, Vol. 46, No. 3-4, 05.03.2010, p. 309-314.Research output: Contribution to journal › Article
TY - JOUR
T1 - Hypersensitive immunoassay by using Escherichia coli outer membrane with autodisplayed Z-domains
AU - Park, Min
AU - Jose, Joachim
AU - Pyun, Jae Chul
PY - 2010/3/5
Y1 - 2010/3/5
N2 - In this work, the Z-domain of protein A was autodisplayed on the surface of the Escherichia coli outer membrane as a fusion protein of AIDA-1, and then layered on a microplate in order to develop a hypersensitive immunoassay. By using microplates with controlled hydrophilicity the formation of outer membrane layer was carried out, and the driving force for the layer formation was proven to be a hydrophobic interactions. Through the orientation control of the immobilized antibodies by using autodisplayed Z-domains, the limit of detection (LOD) and the sensitivity of this new immunoassay were determined to have improved as much as 30-fold compared to the conventional ELISA. The applicability of the new immunoassay for medical diagnosis was demonstrated by the detection of C-reactive protein (CRP) which is known to be a biomarker protein for inflammatory diseases.
AB - In this work, the Z-domain of protein A was autodisplayed on the surface of the Escherichia coli outer membrane as a fusion protein of AIDA-1, and then layered on a microplate in order to develop a hypersensitive immunoassay. By using microplates with controlled hydrophilicity the formation of outer membrane layer was carried out, and the driving force for the layer formation was proven to be a hydrophobic interactions. Through the orientation control of the immobilized antibodies by using autodisplayed Z-domains, the limit of detection (LOD) and the sensitivity of this new immunoassay were determined to have improved as much as 30-fold compared to the conventional ELISA. The applicability of the new immunoassay for medical diagnosis was demonstrated by the detection of C-reactive protein (CRP) which is known to be a biomarker protein for inflammatory diseases.
UR - http://www.scopus.com/inward/record.url?scp=74149086784&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=74149086784&partnerID=8YFLogxK
U2 - 10.1016/j.enzmictec.2009.12.004
DO - 10.1016/j.enzmictec.2009.12.004
M3 - Article
VL - 46
SP - 309
EP - 314
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
SN - 0141-0229
IS - 3-4
ER -