Hypoxia-enhanced wound-healing function of adipose-derived stem cells: Increase in stem cell proliferation and up-regulation of VEGF and bFGF

Eun Young Lee, Ying Xia, Won Serk Kim, Myoung Hee Kim, Tae Hwan Kim, Kea Jeung Kim, Byung Soon Park, Jong Hyuk Sung

Research output: Contribution to journalArticle

248 Citations (Scopus)

Abstract

Adipose-derived stem cells (ADSCs) have been shown to induce wound-healing effects. Because inflammation near the wound area induces oxygen deficiency, it is interesting to elucidate the effect of hypoxia on the function of ADSCs. In this work, we asked: (1) does hypoxia alter the wound-healing function of ADSCs? and (2) what are the major factors responsible for the alteration in the wound-healing function? Effect of hypoxia on the proliferation of ADSCs was first examined that hypoxia (2% O2) enhanced the proliferation of ADSCs in either the presence of serum or in the absence of serum. The conditioned medium of ADSCs harvested under hypoxia (hypoCM) significantly promoted collagen synthesis and the migration of human dermal fibroblasts, compared with that in normoxia (norCM). In the animal studies, hypoCM significantly reduced the wound area compared with norCM. Furthermore, mRNA and protein measurements showed that hypoxia up-regulated growth factors such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF). Inhibition of VEGF and bFGF using neutralizing antibodies reversed the migration of the wounded human dermal fibroblasts and the healing of wounds in animal experiment. Collectively, these results suggest that hypoxia increases the proliferation of ADSCs and enhances the wound-healing function of ADSCs, at least partly, by up-regulating the secretion of VEGF and bFGF.

Original languageEnglish
Pages (from-to)540-547
Number of pages8
JournalWound Repair and Regeneration
Volume17
Issue number4
DOIs
Publication statusPublished - 2009 Jul 1

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Fibroblast Growth Factor 2
Wound Healing
Vascular Endothelial Growth Factor A
Up-Regulation
Stem Cells
Cell Proliferation
Fibroblasts
Skin
Hypoxia
Wounds and Injuries
Conditioned Culture Medium
Neutralizing Antibodies
Serum
Intercellular Signaling Peptides and Proteins
Collagen
Inflammation
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Surgery
  • Dermatology

Cite this

Lee, Eun Young ; Xia, Ying ; Kim, Won Serk ; Kim, Myoung Hee ; Kim, Tae Hwan ; Kim, Kea Jeung ; Park, Byung Soon ; Sung, Jong Hyuk. / Hypoxia-enhanced wound-healing function of adipose-derived stem cells : Increase in stem cell proliferation and up-regulation of VEGF and bFGF. In: Wound Repair and Regeneration. 2009 ; Vol. 17, No. 4. pp. 540-547.
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Hypoxia-enhanced wound-healing function of adipose-derived stem cells : Increase in stem cell proliferation and up-regulation of VEGF and bFGF. / Lee, Eun Young; Xia, Ying; Kim, Won Serk; Kim, Myoung Hee; Kim, Tae Hwan; Kim, Kea Jeung; Park, Byung Soon; Sung, Jong Hyuk.

In: Wound Repair and Regeneration, Vol. 17, No. 4, 01.07.2009, p. 540-547.

Research output: Contribution to journalArticle

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