Hypoxia-induced expression of VEGF in the organotypic spinal cord slice culture

Sung Su An, William A. Pennant, Yoon Ha, Jin Soo Oh, Hyo Jin Kim, So Jung Gwak, Do Heum Yoon, Keung Nyun Kim

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5 Citations (Scopus)

Abstract

We used the erythropoietin enhancer and Simian virus-40 promoter to create a hypoxia-inducible gene expression system to investigate the effect of vascular endothelial growth factor (VEGF) gene therapy on neuroprotection and neurogenesis in organotypic spinal cord slice culture. The organotypic spinal cord slice culture transfected with pEpo-SV-VEGF expressed the highest amount of VEGF under hypoxic conditions and showed decreased apoptosis and increased proliferation, and evidence of neurogenesis. Our results show that the hypoxia-induced VEGF expression in an organotypic spinal cord slice culture may lead to an optimal treatment for spinal cord injury.

Original languageEnglish
Pages (from-to)55-60
Number of pages6
JournalNeuroReport
Volume22
Issue number2
DOIs
Publication statusPublished - 2011 Jan 26

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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  • Cite this

    An, S. S., Pennant, W. A., Ha, Y., Oh, J. S., Kim, H. J., Gwak, S. J., Yoon, D. H., & Kim, K. N. (2011). Hypoxia-induced expression of VEGF in the organotypic spinal cord slice culture. NeuroReport, 22(2), 55-60. https://doi.org/10.1097/WNR.0b013e3283418b00