Hypoxia induces epithelial-mesenchymal transition in follicular thyroid cancer: Involvement of regulation of twist by hypoxia inducible factor-1α

Yeon Ju Yang, Hwi Jung Na, Michelle J. Suh, Myung Jin Ban, Hyung Kwon Byeon, Won Shik Kim, Jae Wook Kim, Eun Chang Choi, Hyeong Ju Kwon, Jae Won Chang, Yoon Woo Koh

Research output: Contribution to journalArticle

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Abstract

Purpose: Although follicular thyroid cancer (FTC) has a relatively fair prognosis, distant metastasis sometimes results in poor prognosis and survival. There is little understanding of the mechanisms contributing to the aggressiveness potential of thyroid cancer. We showed that hypoxia inducible factor-1α (HIF-1α) induced aggressiveness in FTC cells and identified the underlying mechanism of the HIF-1α-induced invasive characteristics. Materials and Methods: Cells were cultured under controlled hypoxic environments (1% O2) or normoxic conditions. The effect of hypoxia on HIF-1α, and epithelial-to-mesenchymal transition (EMT) related markers were evaluated by quantitative real-time PCR, Western blot analysis and immunocytochemistry. Invasion and wound healing assay were conducted to identify functional character of EMT. The involvement of HIF-1α and Twist in EMT were studied using gene overexpression or silencing. After orthotopic nude mouse model was established using the cells transfected with lentiviral shHIF-1α, tissue analysis was done. Results: Hypoxia induces HIF-1α expression and EMT, including typical morphologic changes, cadherin shift, and increased vimentin expression. We showed that overexpression of HIF-1α via transfection resulted in the aforementioned changes without hypoxia, and repression of HIF-1α with RNA interference suppressed hypoxia-induced HIF-1α and EMT. Furthermore, we also observed that Twist expression was regulated by HIF-1α. These were confirmed in the orthotopic FTC model. Conclusion: Hypoxia induced HIF-1α, which in turn induced EMT, resulting in the increased capacity for invasion and migration of cells via regulation of the Twist signal pathway in FTC cells. These findings provide insight into a possible therapeutic strategy to prevent invasive and metastatic FTC.

Original languageEnglish
Pages (from-to)1503-1514
Number of pages12
JournalYonsei medical journal
Volume56
Issue number6
DOIs
Publication statusPublished - 2015 Nov

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Hypoxia-Inducible Factor 1
Epithelial-Mesenchymal Transition
Follicular Thyroid cancer
Hypoxia
Controlled Environment
Vimentin
Cadherins
RNA Interference
Thyroid Neoplasms
Nude Mice
Wound Healing
Cell Movement
Transfection
Real-Time Polymerase Chain Reaction
Cultured Cells
Signal Transduction
Western Blotting
Immunohistochemistry
Neoplasm Metastasis

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Yang, Yeon Ju ; Na, Hwi Jung ; Suh, Michelle J. ; Ban, Myung Jin ; Byeon, Hyung Kwon ; Kim, Won Shik ; Kim, Jae Wook ; Choi, Eun Chang ; Kwon, Hyeong Ju ; Chang, Jae Won ; Koh, Yoon Woo. / Hypoxia induces epithelial-mesenchymal transition in follicular thyroid cancer : Involvement of regulation of twist by hypoxia inducible factor-1α. In: Yonsei medical journal. 2015 ; Vol. 56, No. 6. pp. 1503-1514.
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abstract = "Purpose: Although follicular thyroid cancer (FTC) has a relatively fair prognosis, distant metastasis sometimes results in poor prognosis and survival. There is little understanding of the mechanisms contributing to the aggressiveness potential of thyroid cancer. We showed that hypoxia inducible factor-1α (HIF-1α) induced aggressiveness in FTC cells and identified the underlying mechanism of the HIF-1α-induced invasive characteristics. Materials and Methods: Cells were cultured under controlled hypoxic environments (1{\%} O2) or normoxic conditions. The effect of hypoxia on HIF-1α, and epithelial-to-mesenchymal transition (EMT) related markers were evaluated by quantitative real-time PCR, Western blot analysis and immunocytochemistry. Invasion and wound healing assay were conducted to identify functional character of EMT. The involvement of HIF-1α and Twist in EMT were studied using gene overexpression or silencing. After orthotopic nude mouse model was established using the cells transfected with lentiviral shHIF-1α, tissue analysis was done. Results: Hypoxia induces HIF-1α expression and EMT, including typical morphologic changes, cadherin shift, and increased vimentin expression. We showed that overexpression of HIF-1α via transfection resulted in the aforementioned changes without hypoxia, and repression of HIF-1α with RNA interference suppressed hypoxia-induced HIF-1α and EMT. Furthermore, we also observed that Twist expression was regulated by HIF-1α. These were confirmed in the orthotopic FTC model. Conclusion: Hypoxia induced HIF-1α, which in turn induced EMT, resulting in the increased capacity for invasion and migration of cells via regulation of the Twist signal pathway in FTC cells. These findings provide insight into a possible therapeutic strategy to prevent invasive and metastatic FTC.",
author = "Yang, {Yeon Ju} and Na, {Hwi Jung} and Suh, {Michelle J.} and Ban, {Myung Jin} and Byeon, {Hyung Kwon} and Kim, {Won Shik} and Kim, {Jae Wook} and Choi, {Eun Chang} and Kwon, {Hyeong Ju} and Chang, {Jae Won} and Koh, {Yoon Woo}",
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Hypoxia induces epithelial-mesenchymal transition in follicular thyroid cancer : Involvement of regulation of twist by hypoxia inducible factor-1α. / Yang, Yeon Ju; Na, Hwi Jung; Suh, Michelle J.; Ban, Myung Jin; Byeon, Hyung Kwon; Kim, Won Shik; Kim, Jae Wook; Choi, Eun Chang; Kwon, Hyeong Ju; Chang, Jae Won; Koh, Yoon Woo.

In: Yonsei medical journal, Vol. 56, No. 6, 11.2015, p. 1503-1514.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Hypoxia induces epithelial-mesenchymal transition in follicular thyroid cancer

T2 - Involvement of regulation of twist by hypoxia inducible factor-1α

AU - Yang, Yeon Ju

AU - Na, Hwi Jung

AU - Suh, Michelle J.

AU - Ban, Myung Jin

AU - Byeon, Hyung Kwon

AU - Kim, Won Shik

AU - Kim, Jae Wook

AU - Choi, Eun Chang

AU - Kwon, Hyeong Ju

AU - Chang, Jae Won

AU - Koh, Yoon Woo

PY - 2015/11

Y1 - 2015/11

N2 - Purpose: Although follicular thyroid cancer (FTC) has a relatively fair prognosis, distant metastasis sometimes results in poor prognosis and survival. There is little understanding of the mechanisms contributing to the aggressiveness potential of thyroid cancer. We showed that hypoxia inducible factor-1α (HIF-1α) induced aggressiveness in FTC cells and identified the underlying mechanism of the HIF-1α-induced invasive characteristics. Materials and Methods: Cells were cultured under controlled hypoxic environments (1% O2) or normoxic conditions. The effect of hypoxia on HIF-1α, and epithelial-to-mesenchymal transition (EMT) related markers were evaluated by quantitative real-time PCR, Western blot analysis and immunocytochemistry. Invasion and wound healing assay were conducted to identify functional character of EMT. The involvement of HIF-1α and Twist in EMT were studied using gene overexpression or silencing. After orthotopic nude mouse model was established using the cells transfected with lentiviral shHIF-1α, tissue analysis was done. Results: Hypoxia induces HIF-1α expression and EMT, including typical morphologic changes, cadherin shift, and increased vimentin expression. We showed that overexpression of HIF-1α via transfection resulted in the aforementioned changes without hypoxia, and repression of HIF-1α with RNA interference suppressed hypoxia-induced HIF-1α and EMT. Furthermore, we also observed that Twist expression was regulated by HIF-1α. These were confirmed in the orthotopic FTC model. Conclusion: Hypoxia induced HIF-1α, which in turn induced EMT, resulting in the increased capacity for invasion and migration of cells via regulation of the Twist signal pathway in FTC cells. These findings provide insight into a possible therapeutic strategy to prevent invasive and metastatic FTC.

AB - Purpose: Although follicular thyroid cancer (FTC) has a relatively fair prognosis, distant metastasis sometimes results in poor prognosis and survival. There is little understanding of the mechanisms contributing to the aggressiveness potential of thyroid cancer. We showed that hypoxia inducible factor-1α (HIF-1α) induced aggressiveness in FTC cells and identified the underlying mechanism of the HIF-1α-induced invasive characteristics. Materials and Methods: Cells were cultured under controlled hypoxic environments (1% O2) or normoxic conditions. The effect of hypoxia on HIF-1α, and epithelial-to-mesenchymal transition (EMT) related markers were evaluated by quantitative real-time PCR, Western blot analysis and immunocytochemistry. Invasion and wound healing assay were conducted to identify functional character of EMT. The involvement of HIF-1α and Twist in EMT were studied using gene overexpression or silencing. After orthotopic nude mouse model was established using the cells transfected with lentiviral shHIF-1α, tissue analysis was done. Results: Hypoxia induces HIF-1α expression and EMT, including typical morphologic changes, cadherin shift, and increased vimentin expression. We showed that overexpression of HIF-1α via transfection resulted in the aforementioned changes without hypoxia, and repression of HIF-1α with RNA interference suppressed hypoxia-induced HIF-1α and EMT. Furthermore, we also observed that Twist expression was regulated by HIF-1α. These were confirmed in the orthotopic FTC model. Conclusion: Hypoxia induced HIF-1α, which in turn induced EMT, resulting in the increased capacity for invasion and migration of cells via regulation of the Twist signal pathway in FTC cells. These findings provide insight into a possible therapeutic strategy to prevent invasive and metastatic FTC.

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